The Effect of Feed Contamination with Mycotoxins on Animals and Ways for Prevention and Degradation of Mycotoxins
Mycotoxins are secondary metabolites produced by fungi that are capable of causing illness and sometimes death to animals and not only animals even humans. In 1960 it was established that some fungal metabolites, now called mycotoxins, that have a destructive effect on animal health, since then peop...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/527 |
| _version_ | 1797214943652610048 |
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| author | Oana Ciobotaru Iulian Grosu Calina Petruta Cornea |
| author_facet | Oana Ciobotaru Iulian Grosu Calina Petruta Cornea |
| author_sort | Oana Ciobotaru |
| collection | DOAJ |
| description | Mycotoxins are secondary metabolites produced by fungi that are capable of causing illness and sometimes death to animals and not only animals even humans. In 1960 it was established that some fungal metabolites, now called mycotoxins, that have a destructive effect on animal health, since then people were interested on the effect and the way to stop it. Among them, aflatoxins, B1, B2, G1 & G2 synthesized mainly by Aspergillus flavus/ Aspergillus parasiticus are known to induce severe effects on animal: can cause liver damage, decreased milk production, reduced reproductively and suppressed immunity in animals consuming low dietary concentrations, decreased feed intake and efficiency, weight loss, jaundice, drop in milk production, nervous signs, bleeding and death. The aim of this work was the isolation of aflatoxin producing fungi in order to investigate new ways that can determinate, inhibit or degradation of aflatoxin, ochratoxin, using lactic bacteria and yeast. A number of 14 Aspergillus spp. isolates were selected from wheat, barley, triticale, oats, and sunflower seeds and identified, based on macroscopic and microscopic features as A.flavus/A.parasiticus. The ability of aflatoxin biosynthesis was detected on PDA medium with β cyclodextrine and sodium deoxycholate were evaluated by TLC and RIDA Screen R-biopharm. At this stage of experiments 3 fungal isolates, designated as GE2, G32, T11 were selected as aflatoxin B1, B2, G1 and used for further analysis (molecular identification, interactions with LAB and yeasts). |
| first_indexed | 2024-04-24T11:22:12Z |
| format | Article |
| id | doaj.art-29bb8e342bb2422abfef8c6f22931090 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:22:12Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-29bb8e342bb2422abfef8c6f229310902024-04-11T04:29:17ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-014719494527The Effect of Feed Contamination with Mycotoxins on Animals and Ways for Prevention and Degradation of MycotoxinsOana Ciobotaru0Iulian Grosu1Calina Petruta Cornea2University of Agronomic Sciences and Veterinary Medicine – Bucharest, Faculty of Biotechnologies, 59 Mărăşti Blvd, 011464 Bucharest, RomaniaUniversity of Agronomic Sciences and Veterinary Medicine – Bucharest, Faculty of Biotechnologies, 59 Mărăşti Blvd, 011464 Bucharest, RomaniaUniversity of Agronomic Sciences and Veterinary Medicine – Bucharest, Faculty of Biotechnologies, 59 Mărăşti Blvd, 011464 Bucharest, RomaniaMycotoxins are secondary metabolites produced by fungi that are capable of causing illness and sometimes death to animals and not only animals even humans. In 1960 it was established that some fungal metabolites, now called mycotoxins, that have a destructive effect on animal health, since then people were interested on the effect and the way to stop it. Among them, aflatoxins, B1, B2, G1 & G2 synthesized mainly by Aspergillus flavus/ Aspergillus parasiticus are known to induce severe effects on animal: can cause liver damage, decreased milk production, reduced reproductively and suppressed immunity in animals consuming low dietary concentrations, decreased feed intake and efficiency, weight loss, jaundice, drop in milk production, nervous signs, bleeding and death. The aim of this work was the isolation of aflatoxin producing fungi in order to investigate new ways that can determinate, inhibit or degradation of aflatoxin, ochratoxin, using lactic bacteria and yeast. A number of 14 Aspergillus spp. isolates were selected from wheat, barley, triticale, oats, and sunflower seeds and identified, based on macroscopic and microscopic features as A.flavus/A.parasiticus. The ability of aflatoxin biosynthesis was detected on PDA medium with β cyclodextrine and sodium deoxycholate were evaluated by TLC and RIDA Screen R-biopharm. At this stage of experiments 3 fungal isolates, designated as GE2, G32, T11 were selected as aflatoxin B1, B2, G1 and used for further analysis (molecular identification, interactions with LAB and yeasts).https://spasb.ro/index.php/public_html/article/view/527animal illnessaspergillus flavusmycotoxins |
| spellingShingle | Oana Ciobotaru Iulian Grosu Calina Petruta Cornea The Effect of Feed Contamination with Mycotoxins on Animals and Ways for Prevention and Degradation of Mycotoxins Scientific Papers Animal Science and Biotechnologies animal illness aspergillus flavus mycotoxins |
| title | The Effect of Feed Contamination with Mycotoxins on Animals and Ways for Prevention and Degradation of Mycotoxins |
| title_full | The Effect of Feed Contamination with Mycotoxins on Animals and Ways for Prevention and Degradation of Mycotoxins |
| title_fullStr | The Effect of Feed Contamination with Mycotoxins on Animals and Ways for Prevention and Degradation of Mycotoxins |
| title_full_unstemmed | The Effect of Feed Contamination with Mycotoxins on Animals and Ways for Prevention and Degradation of Mycotoxins |
| title_short | The Effect of Feed Contamination with Mycotoxins on Animals and Ways for Prevention and Degradation of Mycotoxins |
| title_sort | effect of feed contamination with mycotoxins on animals and ways for prevention and degradation of mycotoxins |
| topic | animal illness aspergillus flavus mycotoxins |
| url | https://spasb.ro/index.php/public_html/article/view/527 |
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