Estrogen receptor-related receptors in mandarin fish (Siniperca chuatsi): Molecular cloning, characterization, and estrogen responsiveness
The estrogen-related receptors (ERRs), a group of nuclear receptors, are the first orphan receptor to be identified. In this study, six errs (errα, errβ, errγ, errγA, errγB, errδ) were first cloned from the gonads of mandarin fish (Siniperca chuatsi). Gene cloning results showed the open reading fra...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513422001338 |
| _version_ | 1818232300168544256 |
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| author | Shiyan Liu Haofeng Ouyang Chong Han Jingjun Huang Qiaoying Zhu Dingrui Liu Linqiang Han Shuisheng Li Guifeng Li Haoran Lin Yong Zhang |
| author_facet | Shiyan Liu Haofeng Ouyang Chong Han Jingjun Huang Qiaoying Zhu Dingrui Liu Linqiang Han Shuisheng Li Guifeng Li Haoran Lin Yong Zhang |
| author_sort | Shiyan Liu |
| collection | DOAJ |
| description | The estrogen-related receptors (ERRs), a group of nuclear receptors, are the first orphan receptor to be identified. In this study, six errs (errα, errβ, errγ, errγA, errγB, errδ) were first cloned from the gonads of mandarin fish (Siniperca chuatsi). Gene cloning results showed the open reading frames (ORF) of both errα, errβ, errγ, errγA, errγB, errδ were 1302 bp, 1434 bp, 1323 bp, 1566 bp and 1308 bp, coding a 432, 476, 439, 520, 434 and 337 amino acid (AA) peptide, respectively. Tissue distribution analysis showed that errα was relatively highly expressed in heart and muscle both in male and female and errγA was highly expressed in brain, stomach and muscle while the errγB was extremely highly expressed in heart and liver both in male and female. The errβ expressed relatively highly in medulla oblongata and gonad of male and female and kidney of male, while errγ expressed relatively highly in stomach of male and female. Gonad expression pattern revealed that errα, errγ, errγA and errγB were all detected from 5 to 180 day after hatching (dah) in both males and females, and errδ began to express after 60 dah. Moreover, errγ, errγA and errγB were highly expressed in 5–10 dah while errα and errδ were mainly expressed in 60–180 dah. Furthermore, luciferase assay showed that errγ could response to estrogen stimulation by modulating estrogen-signaling pathway in mandarin fish. |
| first_indexed | 2024-12-12T11:04:05Z |
| format | Article |
| id | doaj.art-29bbfaadfaed4510b3483c1b74c6f287 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-12T11:04:05Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-29bbfaadfaed4510b3483c1b74c6f2872022-12-22T00:26:27ZengElsevierAquaculture Reports2352-51342022-06-0124101137Estrogen receptor-related receptors in mandarin fish (Siniperca chuatsi): Molecular cloning, characterization, and estrogen responsivenessShiyan Liu0Haofeng Ouyang1Chong Han2Jingjun Huang3Qiaoying Zhu4Dingrui Liu5Linqiang Han6Shuisheng Li7Guifeng Li8Haoran Lin9Yong Zhang10State Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266373, ChinaState Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266373, ChinaState Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, China; Guangdong Liangshi Aquatic Seed Industry Co., Ltd, Foshan 528100, ChinaState Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266373, ChinaState Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, ChinaGuangdong Liangshi Aquatic Seed Industry Co., Ltd, Foshan 528100, ChinaState Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, China; Corresponding author.State Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, ChinaState Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266373, ChinaState Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266373, China; Guangdong Liangshi Aquatic Seed Industry Co., Ltd, Foshan 528100, China; Corresponding author at: State Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou 510275, China.The estrogen-related receptors (ERRs), a group of nuclear receptors, are the first orphan receptor to be identified. In this study, six errs (errα, errβ, errγ, errγA, errγB, errδ) were first cloned from the gonads of mandarin fish (Siniperca chuatsi). Gene cloning results showed the open reading frames (ORF) of both errα, errβ, errγ, errγA, errγB, errδ were 1302 bp, 1434 bp, 1323 bp, 1566 bp and 1308 bp, coding a 432, 476, 439, 520, 434 and 337 amino acid (AA) peptide, respectively. Tissue distribution analysis showed that errα was relatively highly expressed in heart and muscle both in male and female and errγA was highly expressed in brain, stomach and muscle while the errγB was extremely highly expressed in heart and liver both in male and female. The errβ expressed relatively highly in medulla oblongata and gonad of male and female and kidney of male, while errγ expressed relatively highly in stomach of male and female. Gonad expression pattern revealed that errα, errγ, errγA and errγB were all detected from 5 to 180 day after hatching (dah) in both males and females, and errδ began to express after 60 dah. Moreover, errγ, errγA and errγB were highly expressed in 5–10 dah while errα and errδ were mainly expressed in 60–180 dah. Furthermore, luciferase assay showed that errγ could response to estrogen stimulation by modulating estrogen-signaling pathway in mandarin fish.http://www.sciencedirect.com/science/article/pii/S2352513422001338Estrogen-related receptorsEstrogen receptorEstrogen response elementsGene expressionMandarin fish |
| spellingShingle | Shiyan Liu Haofeng Ouyang Chong Han Jingjun Huang Qiaoying Zhu Dingrui Liu Linqiang Han Shuisheng Li Guifeng Li Haoran Lin Yong Zhang Estrogen receptor-related receptors in mandarin fish (Siniperca chuatsi): Molecular cloning, characterization, and estrogen responsiveness Aquaculture Reports Estrogen-related receptors Estrogen receptor Estrogen response elements Gene expression Mandarin fish |
| title | Estrogen receptor-related receptors in mandarin fish (Siniperca chuatsi): Molecular cloning, characterization, and estrogen responsiveness |
| title_full | Estrogen receptor-related receptors in mandarin fish (Siniperca chuatsi): Molecular cloning, characterization, and estrogen responsiveness |
| title_fullStr | Estrogen receptor-related receptors in mandarin fish (Siniperca chuatsi): Molecular cloning, characterization, and estrogen responsiveness |
| title_full_unstemmed | Estrogen receptor-related receptors in mandarin fish (Siniperca chuatsi): Molecular cloning, characterization, and estrogen responsiveness |
| title_short | Estrogen receptor-related receptors in mandarin fish (Siniperca chuatsi): Molecular cloning, characterization, and estrogen responsiveness |
| title_sort | estrogen receptor related receptors in mandarin fish siniperca chuatsi molecular cloning characterization and estrogen responsiveness |
| topic | Estrogen-related receptors Estrogen receptor Estrogen response elements Gene expression Mandarin fish |
| url | http://www.sciencedirect.com/science/article/pii/S2352513422001338 |
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