A linear DNA template-based framework for site-specific unnatural amino acid incorporation

A linear DNA template-based framework for site-specific unnatural amino acid incorporation

Site-specific incorporation of unnatural amino acids (UNAAs) into proteins using an orthogonal translation system (OTS) has expanded the scope of protein-coding chemistry. The key factor affecting UNAA embedding efficiency is the orthogonality of the OTS. Compared to traditional cell systems, cell-f...

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Bibliographic Details
Main Authors: Xinjie Chen, Yingying Liu, Jiaqi Hou, Yuan Lu
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2021-09-01
Series:Synthetic and Systems Biotechnology
Subjects:
Cell-free protein synthesis
Linear PCR product
Rapid screening
Unnatural amino acids
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X21000399

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