Effects of anoxic prognostic model on immune microenvironment in pancreatic cancer
Abstract Pancreatic cancer has one of the worst prognoses in the world, which suggests that the tumor microenvironment, which is characterized by hypoxia and immunosuppression, plays a significant role in the prognosis and progression of pancreatic cancer. We identified PLAU, LDHA, and PKM as key ge...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-36413-9 |
| _version_ | 1827928688978558976 |
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| author | Yangdong Wu Qingyan Kou Lin Sun Xiao Hu |
| author_facet | Yangdong Wu Qingyan Kou Lin Sun Xiao Hu |
| author_sort | Yangdong Wu |
| collection | DOAJ |
| description | Abstract Pancreatic cancer has one of the worst prognoses in the world, which suggests that the tumor microenvironment, which is characterized by hypoxia and immunosuppression, plays a significant role in the prognosis and progression of pancreatic cancer. We identified PLAU, LDHA, and PKM as key genes involved in pancreatic cancer hypoxia through GO/KEGG enrichment related hypoxia pathways and cox regression, established prognostic models, and studied their relationship to immune invasion through bioinformatics using R and related online databases. We verified the high expression of PLAU, LDHA, and PKM in pancreatic cancer cells using qPCR in vitro, and we also discovered that the expression of PLAU, LDHA, and PKM in hypoxic pancreatic cancer cells differed from that in normal cultured pancreatic cancer cells. Finally, we discovered that our prognostic model accurately predicted postrain in pancreatic cancer patients with hypoxia and immune infiltration. |
| first_indexed | 2024-03-13T06:12:16Z |
| format | Article |
| id | doaj.art-29becefeff37487f9cb6a709a8a36edf |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T06:12:16Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-29becefeff37487f9cb6a709a8a36edf2023-06-11T11:11:59ZengNature PortfolioScientific Reports2045-23222023-06-0113111110.1038/s41598-023-36413-9Effects of anoxic prognostic model on immune microenvironment in pancreatic cancerYangdong Wu0Qingyan Kou1Lin Sun2Xiao Hu3Department of Hepatobiliary Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityDepartment of ICU, The Affiliated Hospital of Qingdao UniversityDepartment of Hepatobiliary Pancreatic Surgery, The Affiliated Hospital of Qingdao UniversityAbstract Pancreatic cancer has one of the worst prognoses in the world, which suggests that the tumor microenvironment, which is characterized by hypoxia and immunosuppression, plays a significant role in the prognosis and progression of pancreatic cancer. We identified PLAU, LDHA, and PKM as key genes involved in pancreatic cancer hypoxia through GO/KEGG enrichment related hypoxia pathways and cox regression, established prognostic models, and studied their relationship to immune invasion through bioinformatics using R and related online databases. We verified the high expression of PLAU, LDHA, and PKM in pancreatic cancer cells using qPCR in vitro, and we also discovered that the expression of PLAU, LDHA, and PKM in hypoxic pancreatic cancer cells differed from that in normal cultured pancreatic cancer cells. Finally, we discovered that our prognostic model accurately predicted postrain in pancreatic cancer patients with hypoxia and immune infiltration.https://doi.org/10.1038/s41598-023-36413-9 |
| spellingShingle | Yangdong Wu Qingyan Kou Lin Sun Xiao Hu Effects of anoxic prognostic model on immune microenvironment in pancreatic cancer Scientific Reports |
| title | Effects of anoxic prognostic model on immune microenvironment in pancreatic cancer |
| title_full | Effects of anoxic prognostic model on immune microenvironment in pancreatic cancer |
| title_fullStr | Effects of anoxic prognostic model on immune microenvironment in pancreatic cancer |
| title_full_unstemmed | Effects of anoxic prognostic model on immune microenvironment in pancreatic cancer |
| title_short | Effects of anoxic prognostic model on immune microenvironment in pancreatic cancer |
| title_sort | effects of anoxic prognostic model on immune microenvironment in pancreatic cancer |
| url | https://doi.org/10.1038/s41598-023-36413-9 |
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