Binding indocyanine green to human serum albumin potentially enhances the detection of sentinel lymph nodes. An initial step for facilitating the detection of first-station nodes in penile and other urological cancers
Introduction Surgical oncology strives to remove the primary cancer tumor together with its local lymphatic tissue. One of the techniques improving the staging of lymph nodes is sentinel node biopsy. The most common agent used in SNB is indocyanine green (ICG). Indocyanine green is characterized by...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2021-01-01
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| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/Binding-indocyanine-green-to-human-serum-albumin-potentially-enhances-the-detection,113237,0,2.html |
| _version_ | 1828221348182228992 |
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| author | Marcin Markuszewski Magdalena Buszewska-Forajta Małgorzata Artymowicz Wojciech Połom Marek Roslan Michał Markuszewski |
| author_facet | Marcin Markuszewski Magdalena Buszewska-Forajta Małgorzata Artymowicz Wojciech Połom Marek Roslan Michał Markuszewski |
| author_sort | Marcin Markuszewski |
| collection | DOAJ |
| description | Introduction
Surgical oncology strives to remove the primary cancer tumor together with its local lymphatic tissue. One of the techniques improving the staging of lymph nodes is sentinel node biopsy. The most common agent used in SNB is indocyanine green (ICG). Indocyanine green is characterized by its high affinity for human serum albumin (HSA). In practice, the visualization of the sentinel node is enhanced by attaching a relatively large carrier to the ICG molecule. The aim of this study was to investigate whether the covalent linking of ICG to a nanocolloid would extend the time of detection of the dye as it binds to HSA, assessed by fluorescence measurements in vitro.
Material and methods
The influence of the molar concentration of ICG on its ability to form a complex with HSA was investigated. The dye luminescence was measured, with an increasing amount of dye in the presence of a constant concentration of HSA. The stability of the ICG:HSA complex was also investigated.
Results
The binding of ICG and human protein in a solution ratio of 3 : 1 made it possible to detect the ICG luminescence with better and prolonged visibility. In the case of the two lowest ratios, complex formation was not observed. The use of ICG bound to a nanocolloid based on human serum albumin increases the luminescence of the HSA:ICG complex up to 98%.
Conclusions
Properly selected proportions of human albumin protein and ICH allowed higher and longer luminescence to be achieved. Nevertheless, further studies are necessary to establish the optimal concentration ratio. |
| first_indexed | 2024-04-12T16:40:09Z |
| format | Article |
| id | doaj.art-29c0cf6b8e6d451f801130cd18859113 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-04-12T16:40:09Z |
| publishDate | 2021-01-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-29c0cf6b8e6d451f801130cd188591132022-12-22T03:24:50ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512021-01-0118371972510.5114/aoms/113237113237Binding indocyanine green to human serum albumin potentially enhances the detection of sentinel lymph nodes. An initial step for facilitating the detection of first-station nodes in penile and other urological cancersMarcin Markuszewski0Magdalena Buszewska-Forajta1Małgorzata Artymowicz2Wojciech Połom3Marek Roslan4Michał Markuszewski5Department of Urology, Faculty of Medicine, Medical University of Gdansk, Gdansk, PolandDepartment of Biopharmacy and Pharmacokinetics, Faculty of Pharmacy, Medical University of Gdansk, Gdansk, PolandDepartment of Pharmacodynamics, Faculty of Pharmacy, Medical University of Gdansk, Gdansk, PolandDepartment of Urology, Faculty of Medicine, Medical University of Gdansk, Gdansk, PolandDepartment of Urology, Faculty of Medicine, University of Warmia and Mazury, Olsztyn, PolandDepartment of Biopharmacy and Pharmacokinetics, Faculty of Pharmacy, Medical University of Gdansk, Gdansk, PolandIntroduction Surgical oncology strives to remove the primary cancer tumor together with its local lymphatic tissue. One of the techniques improving the staging of lymph nodes is sentinel node biopsy. The most common agent used in SNB is indocyanine green (ICG). Indocyanine green is characterized by its high affinity for human serum albumin (HSA). In practice, the visualization of the sentinel node is enhanced by attaching a relatively large carrier to the ICG molecule. The aim of this study was to investigate whether the covalent linking of ICG to a nanocolloid would extend the time of detection of the dye as it binds to HSA, assessed by fluorescence measurements in vitro. Material and methods The influence of the molar concentration of ICG on its ability to form a complex with HSA was investigated. The dye luminescence was measured, with an increasing amount of dye in the presence of a constant concentration of HSA. The stability of the ICG:HSA complex was also investigated. Results The binding of ICG and human protein in a solution ratio of 3 : 1 made it possible to detect the ICG luminescence with better and prolonged visibility. In the case of the two lowest ratios, complex formation was not observed. The use of ICG bound to a nanocolloid based on human serum albumin increases the luminescence of the HSA:ICG complex up to 98%. Conclusions Properly selected proportions of human albumin protein and ICH allowed higher and longer luminescence to be achieved. Nevertheless, further studies are necessary to establish the optimal concentration ratio.https://www.archivesofmedicalscience.com/Binding-indocyanine-green-to-human-serum-albumin-potentially-enhances-the-detection,113237,0,2.htmlsentinel lymph nodehuman serum albuminindocyanine greenurological cancers |
| spellingShingle | Marcin Markuszewski Magdalena Buszewska-Forajta Małgorzata Artymowicz Wojciech Połom Marek Roslan Michał Markuszewski Binding indocyanine green to human serum albumin potentially enhances the detection of sentinel lymph nodes. An initial step for facilitating the detection of first-station nodes in penile and other urological cancers Archives of Medical Science sentinel lymph node human serum albumin indocyanine green urological cancers |
| title | Binding indocyanine green to human serum albumin potentially enhances the detection of sentinel lymph nodes. An initial step for facilitating the detection of first-station nodes in penile
and other urological cancers |
| title_full | Binding indocyanine green to human serum albumin potentially enhances the detection of sentinel lymph nodes. An initial step for facilitating the detection of first-station nodes in penile
and other urological cancers |
| title_fullStr | Binding indocyanine green to human serum albumin potentially enhances the detection of sentinel lymph nodes. An initial step for facilitating the detection of first-station nodes in penile
and other urological cancers |
| title_full_unstemmed | Binding indocyanine green to human serum albumin potentially enhances the detection of sentinel lymph nodes. An initial step for facilitating the detection of first-station nodes in penile
and other urological cancers |
| title_short | Binding indocyanine green to human serum albumin potentially enhances the detection of sentinel lymph nodes. An initial step for facilitating the detection of first-station nodes in penile
and other urological cancers |
| title_sort | binding indocyanine green to human serum albumin potentially enhances the detection of sentinel lymph nodes an initial step for facilitating the detection of first station nodes in penile and other urological cancers |
| topic | sentinel lymph node human serum albumin indocyanine green urological cancers |
| url | https://www.archivesofmedicalscience.com/Binding-indocyanine-green-to-human-serum-albumin-potentially-enhances-the-detection,113237,0,2.html |
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