Antioxidant and Wound Healing Potential of <i>Vitis vinifera</i> Seeds Supported by Phytochemical Characterization and Docking Studies
This study explored the in vivo wound healing potential of <i>Vitis vinifera</i> seed extract using an excision wound model with focus on wound healing molecular targets including TGFBR1, VEGF, TNF-<i>α</i>, and IL-1<i>β</i>. The wound healing results revealed tha...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/5/881 |
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| author | Tarfah Al-Warhi Eman Maher Zahran Samy Selim Mohammad M. Al-Sanea Mohammed M. Ghoneim Sherif A. Maher Yaser A. Mostafa Faisal Alsenani Mahmoud A. Elrehany Mohammed S. Almuhayawi Soad K. Al Jaouni Usama Ramadan Abdelmohsen Abeer H. Elmaidomy |
| author_facet | Tarfah Al-Warhi Eman Maher Zahran Samy Selim Mohammad M. Al-Sanea Mohammed M. Ghoneim Sherif A. Maher Yaser A. Mostafa Faisal Alsenani Mahmoud A. Elrehany Mohammed S. Almuhayawi Soad K. Al Jaouni Usama Ramadan Abdelmohsen Abeer H. Elmaidomy |
| author_sort | Tarfah Al-Warhi |
| collection | DOAJ |
| description | This study explored the in vivo wound healing potential of <i>Vitis vinifera</i> seed extract using an excision wound model with focus on wound healing molecular targets including TGFBR1, VEGF, TNF-<i>α</i>, and IL-1<i>β</i>. The wound healing results revealed that <i>V. vinifera</i> seed extract enhanced wound closure rates (<i>p</i> < 0.001), elevated TGF-<i>β</i> and VEGF levels, and significantly downregulated TNF-<i>α</i> and IL-1<i>β</i> levels in comparison to the Mebo<sup>®</sup>-treated group. The phenotypical results were supported by biochemical and histopathological findings. Phytochemical investigation yielded a total of 36 compounds including twenty-seven compounds (<b>1</b>–<b>27</b>) identified from seed oil using GC-MS analysis, along with nine isolated compounds. Among the isolated compounds, one new benzofuran dimer (<b>28</b>) along with eight known ones (<b>29</b>–<b>36</b>) were identified. The structure of new compound was elucidated utilizing 1D/2D NMR, with HRESIMS analyses. Moreover, molecular docking experiments were performed to elucidate the molecular targets (TNF-<i>α</i>, TGFBR1, and IL-1<i>β</i>) of the observed wound healing activity. Additionally, the in vitro antioxidant activity of <i>V. vinifera</i> seed extract along with two isolated compounds (ursolic acid <b>34</b>, and <i>β</i>-sitosterol-3-<i>O</i>-glucopyranoside <b>36</b>) were explored. Our study highlights the potential of <i>V. vinifera</i> seed extract in wound repair uncovering the most probable mechanisms of action using in silico analysis. |
| first_indexed | 2024-03-10T03:26:05Z |
| format | Article |
| id | doaj.art-29c155fb19cf41ed95234b512bba605c |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:26:05Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-29c155fb19cf41ed95234b512bba605c2023-11-23T09:51:10ZengMDPI AGAntioxidants2076-39212022-04-0111588110.3390/antiox11050881Antioxidant and Wound Healing Potential of <i>Vitis vinifera</i> Seeds Supported by Phytochemical Characterization and Docking StudiesTarfah Al-Warhi0Eman Maher Zahran1Samy Selim2Mohammad M. Al-Sanea3Mohammed M. Ghoneim4Sherif A. Maher5Yaser A. Mostafa6Faisal Alsenani7Mahmoud A. Elrehany8Mohammed S. Almuhayawi9Soad K. Al Jaouni10Usama Ramadan Abdelmohsen11Abeer H. Elmaidomy12Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72341, Saudi ArabiaPharmaceutical Chemistry Department, College of Pharmacy, Jouf University, Sakaka 72341, Saudi ArabiaDepartment of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Riyadh 13713, Saudi ArabiaDepartment of Biochemistry, Faculty of pharmacy, Deraya University, New Minia City 61111, EgyptPharmaceutical Organic Chemistry Department, Faculty of Pharmacy, Assiut University, Assiut 71526, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Biochemistry, Faculty of pharmacy, Deraya University, New Minia City 61111, EgyptDepartment of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Hematology/Pediatric Oncology, Yousef Abdulatif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, EgyptThis study explored the in vivo wound healing potential of <i>Vitis vinifera</i> seed extract using an excision wound model with focus on wound healing molecular targets including TGFBR1, VEGF, TNF-<i>α</i>, and IL-1<i>β</i>. The wound healing results revealed that <i>V. vinifera</i> seed extract enhanced wound closure rates (<i>p</i> < 0.001), elevated TGF-<i>β</i> and VEGF levels, and significantly downregulated TNF-<i>α</i> and IL-1<i>β</i> levels in comparison to the Mebo<sup>®</sup>-treated group. The phenotypical results were supported by biochemical and histopathological findings. Phytochemical investigation yielded a total of 36 compounds including twenty-seven compounds (<b>1</b>–<b>27</b>) identified from seed oil using GC-MS analysis, along with nine isolated compounds. Among the isolated compounds, one new benzofuran dimer (<b>28</b>) along with eight known ones (<b>29</b>–<b>36</b>) were identified. The structure of new compound was elucidated utilizing 1D/2D NMR, with HRESIMS analyses. Moreover, molecular docking experiments were performed to elucidate the molecular targets (TNF-<i>α</i>, TGFBR1, and IL-1<i>β</i>) of the observed wound healing activity. Additionally, the in vitro antioxidant activity of <i>V. vinifera</i> seed extract along with two isolated compounds (ursolic acid <b>34</b>, and <i>β</i>-sitosterol-3-<i>O</i>-glucopyranoside <b>36</b>) were explored. Our study highlights the potential of <i>V. vinifera</i> seed extract in wound repair uncovering the most probable mechanisms of action using in silico analysis.https://www.mdpi.com/2076-3921/11/5/881<i>Vitis vinifera</i>benzofuranwound healingTNF-<i>α</i>TGF-<i>β</i>in silico |
| spellingShingle | Tarfah Al-Warhi Eman Maher Zahran Samy Selim Mohammad M. Al-Sanea Mohammed M. Ghoneim Sherif A. Maher Yaser A. Mostafa Faisal Alsenani Mahmoud A. Elrehany Mohammed S. Almuhayawi Soad K. Al Jaouni Usama Ramadan Abdelmohsen Abeer H. Elmaidomy Antioxidant and Wound Healing Potential of <i>Vitis vinifera</i> Seeds Supported by Phytochemical Characterization and Docking Studies Antioxidants <i>Vitis vinifera</i> benzofuran wound healing TNF-<i>α</i> TGF-<i>β</i> in silico |
| title | Antioxidant and Wound Healing Potential of <i>Vitis vinifera</i> Seeds Supported by Phytochemical Characterization and Docking Studies |
| title_full | Antioxidant and Wound Healing Potential of <i>Vitis vinifera</i> Seeds Supported by Phytochemical Characterization and Docking Studies |
| title_fullStr | Antioxidant and Wound Healing Potential of <i>Vitis vinifera</i> Seeds Supported by Phytochemical Characterization and Docking Studies |
| title_full_unstemmed | Antioxidant and Wound Healing Potential of <i>Vitis vinifera</i> Seeds Supported by Phytochemical Characterization and Docking Studies |
| title_short | Antioxidant and Wound Healing Potential of <i>Vitis vinifera</i> Seeds Supported by Phytochemical Characterization and Docking Studies |
| title_sort | antioxidant and wound healing potential of i vitis vinifera i seeds supported by phytochemical characterization and docking studies |
| topic | <i>Vitis vinifera</i> benzofuran wound healing TNF-<i>α</i> TGF-<i>β</i> in silico |
| url | https://www.mdpi.com/2076-3921/11/5/881 |
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