Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio
Cyprinid herpesvirus 2 (CyHV-2) causes herpesviral hematopoietic necrosis (HVHN) disease outbreaks in farmed Cyprinid fish, which leads to serious economic losses worldwide. Although oral vaccination is considered the most suitable strategy for preventing infectious diseases in farmed fish, so far t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.932722/full |
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| author | Zhao-Ran Dong Qing-Jiang Mu Wei-Guang Kong Da-Cheng Qin Yong Zhou Xin-You Wang Gao-Feng Cheng Yang-Zhi Luo Tao-Shan Ai Zhen Xu Zhen Xu |
| author_facet | Zhao-Ran Dong Qing-Jiang Mu Wei-Guang Kong Da-Cheng Qin Yong Zhou Xin-You Wang Gao-Feng Cheng Yang-Zhi Luo Tao-Shan Ai Zhen Xu Zhen Xu |
| author_sort | Zhao-Ran Dong |
| collection | DOAJ |
| description | Cyprinid herpesvirus 2 (CyHV-2) causes herpesviral hematopoietic necrosis (HVHN) disease outbreaks in farmed Cyprinid fish, which leads to serious economic losses worldwide. Although oral vaccination is considered the most suitable strategy for preventing infectious diseases in farmed fish, so far there is no commercial oral vaccine available for controlling HVNN in gibel carp (C. auratus gibelio). In the present study, we developed for the first time an oral vaccine against CyHV-2 by using yeast cell surface display technology and then investigated the effect of this vaccine in gibel carp. Furthermore, the protective efficacy was evaluated by comparing the immune response of a single vaccination with that of a booster vaccination (booster-vaccinated once 2 weeks after the initial vaccination). Critically, the activities of immune-related enzymes and genes expression in vaccine group, especially in the booster vaccine group, were higher than those in the control group. Moreover, strong innate and adaptive immune responses could be elicited in both mucosal and systemic tissues after receipt of the oral yeast vaccine. To further understand the protective efficacy of this vaccine in gibel carp, we successfully developed the challenge model with CyHV-2. Our results showed the relative percent survival was 66.7% in the booster vaccine group, indicating this oral yeast vaccine is a promising vaccine for controlling CyHV-2 disease in gibel carp aquaculture. |
| first_indexed | 2024-12-11T16:26:24Z |
| format | Article |
| id | doaj.art-29c2511522d04e7f8de3c272b5a8a09e |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-11T16:26:24Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-29c2511522d04e7f8de3c272b5a8a09e2022-12-22T00:58:43ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-07-011310.3389/fimmu.2022.932722932722Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelioZhao-Ran Dong0Qing-Jiang Mu1Wei-Guang Kong2Da-Cheng Qin3Yong Zhou4Xin-You Wang5Gao-Feng Cheng6Yang-Zhi Luo7Tao-Shan Ai8Zhen Xu9Zhen Xu10Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaWuhan Chopper Fishery Bio-Tech Co., Ltd, Wuhan Academy of Agricultural Science, Wuhan, ChinaWuhan Chopper Fishery Bio-Tech Co., Ltd, Wuhan Academy of Agricultural Science, Wuhan, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCyprinid herpesvirus 2 (CyHV-2) causes herpesviral hematopoietic necrosis (HVHN) disease outbreaks in farmed Cyprinid fish, which leads to serious economic losses worldwide. Although oral vaccination is considered the most suitable strategy for preventing infectious diseases in farmed fish, so far there is no commercial oral vaccine available for controlling HVNN in gibel carp (C. auratus gibelio). In the present study, we developed for the first time an oral vaccine against CyHV-2 by using yeast cell surface display technology and then investigated the effect of this vaccine in gibel carp. Furthermore, the protective efficacy was evaluated by comparing the immune response of a single vaccination with that of a booster vaccination (booster-vaccinated once 2 weeks after the initial vaccination). Critically, the activities of immune-related enzymes and genes expression in vaccine group, especially in the booster vaccine group, were higher than those in the control group. Moreover, strong innate and adaptive immune responses could be elicited in both mucosal and systemic tissues after receipt of the oral yeast vaccine. To further understand the protective efficacy of this vaccine in gibel carp, we successfully developed the challenge model with CyHV-2. Our results showed the relative percent survival was 66.7% in the booster vaccine group, indicating this oral yeast vaccine is a promising vaccine for controlling CyHV-2 disease in gibel carp aquaculture.https://www.frontiersin.org/articles/10.3389/fimmu.2022.932722/fullCyHV-2gibel carpyeast surface displayoral vaccinationprotective efficacymucosal immune response |
| spellingShingle | Zhao-Ran Dong Qing-Jiang Mu Wei-Guang Kong Da-Cheng Qin Yong Zhou Xin-You Wang Gao-Feng Cheng Yang-Zhi Luo Tao-Shan Ai Zhen Xu Zhen Xu Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio Frontiers in Immunology CyHV-2 gibel carp yeast surface display oral vaccination protective efficacy mucosal immune response |
| title | Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio |
| title_full | Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio |
| title_fullStr | Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio |
| title_full_unstemmed | Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio |
| title_short | Gut mucosal immune responses and protective efficacy of oral yeast Cyprinid herpesvirus 2 (CyHV-2) vaccine in Carassius auratus gibelio |
| title_sort | gut mucosal immune responses and protective efficacy of oral yeast cyprinid herpesvirus 2 cyhv 2 vaccine in carassius auratus gibelio |
| topic | CyHV-2 gibel carp yeast surface display oral vaccination protective efficacy mucosal immune response |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.932722/full |
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