Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae <i>Phaeodactylum tricornutum</i> Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass
Polyunsaturated fatty acids (PUFAs) are essential for human function, however they have to be provided through the diet. As their production from fish oil is environmentally unsustainable, there is demand for new sources of PUFAs. The aim of the present work was to establish the microalgal platform...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/2/119 |
| _version_ | 1798040251210399744 |
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| author | Alok Patel Leonidas Matsakas Kateřina Hrůzová Ulrika Rova Paul Christakopoulos |
| author_facet | Alok Patel Leonidas Matsakas Kateřina Hrůzová Ulrika Rova Paul Christakopoulos |
| author_sort | Alok Patel |
| collection | DOAJ |
| description | Polyunsaturated fatty acids (PUFAs) are essential for human function, however they have to be provided through the diet. As their production from fish oil is environmentally unsustainable, there is demand for new sources of PUFAs. The aim of the present work was to establish the microalgal platform to produce nutraceutical-value PUFAs from forest biomass. To this end, the growth of <i>Phaeodactylum tricornutum</i> on birch and spruce hydrolysates was compared to autotrophic cultivation and glucose synthetic media. Total lipid generated by <i>P. tricornutum</i> grown mixotrophically on glucose, birch, and spruce hydrolysates was 1.21, 1.26, and 1.29 g/L, respectively. The highest eicosapentaenoic acid (EPA) production (256 mg/L) and productivity (19.69 mg/L/d) were observed on spruce hydrolysates. These values were considerably higher than those obtained from the cultivation without glucose (79.80 mg/L and 6.14 mg/L/d, respectively) and also from the photoautotrophic cultivation (26.86 mg/L and 2.44 mg/L/d, respectively). To the best of our knowledge, this is the first report describing the use of forest biomass as raw material for EPA and docosapentaenoic acid (DHA) production. |
| first_indexed | 2024-04-11T22:04:53Z |
| format | Article |
| id | doaj.art-29c2df54e3534281b4de0a327c49f9fb |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T22:04:53Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-29c2df54e3534281b4de0a327c49f9fb2022-12-22T04:00:44ZengMDPI AGMarine Drugs1660-33972019-02-0117211910.3390/md17020119md17020119Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae <i>Phaeodactylum tricornutum</i> Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce BiomassAlok Patel0Leonidas Matsakas1Kateřina Hrůzová2Ulrika Rova3Paul Christakopoulos4Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971-87 Luleå, SwedenBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971-87 Luleå, SwedenBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971-87 Luleå, SwedenBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971-87 Luleå, SwedenBiochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, 971-87 Luleå, SwedenPolyunsaturated fatty acids (PUFAs) are essential for human function, however they have to be provided through the diet. As their production from fish oil is environmentally unsustainable, there is demand for new sources of PUFAs. The aim of the present work was to establish the microalgal platform to produce nutraceutical-value PUFAs from forest biomass. To this end, the growth of <i>Phaeodactylum tricornutum</i> on birch and spruce hydrolysates was compared to autotrophic cultivation and glucose synthetic media. Total lipid generated by <i>P. tricornutum</i> grown mixotrophically on glucose, birch, and spruce hydrolysates was 1.21, 1.26, and 1.29 g/L, respectively. The highest eicosapentaenoic acid (EPA) production (256 mg/L) and productivity (19.69 mg/L/d) were observed on spruce hydrolysates. These values were considerably higher than those obtained from the cultivation without glucose (79.80 mg/L and 6.14 mg/L/d, respectively) and also from the photoautotrophic cultivation (26.86 mg/L and 2.44 mg/L/d, respectively). To the best of our knowledge, this is the first report describing the use of forest biomass as raw material for EPA and docosapentaenoic acid (DHA) production.https://www.mdpi.com/1660-3397/17/2/119polyunsaturated fatty acidsEPADHAmarine algae<i>Phaeodactylum tricornutum</i>forest biomass |
| spellingShingle | Alok Patel Leonidas Matsakas Kateřina Hrůzová Ulrika Rova Paul Christakopoulos Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae <i>Phaeodactylum tricornutum</i> Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass Marine Drugs polyunsaturated fatty acids EPA DHA marine algae <i>Phaeodactylum tricornutum</i> forest biomass |
| title | Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae <i>Phaeodactylum tricornutum</i> Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass |
| title_full | Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae <i>Phaeodactylum tricornutum</i> Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass |
| title_fullStr | Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae <i>Phaeodactylum tricornutum</i> Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass |
| title_full_unstemmed | Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae <i>Phaeodactylum tricornutum</i> Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass |
| title_short | Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae <i>Phaeodactylum tricornutum</i> Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass |
| title_sort | biosynthesis of nutraceutical fatty acids by the oleaginous marine microalgae i phaeodactylum tricornutum i utilizing hydrolysates from organosolv pretreated birch and spruce biomass |
| topic | polyunsaturated fatty acids EPA DHA marine algae <i>Phaeodactylum tricornutum</i> forest biomass |
| url | https://www.mdpi.com/1660-3397/17/2/119 |
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