Artificial Polyploidization Enhances Morphological, Physiological, and Biological Characteristics in <i>Melothria scabra</i> Naudin
Cucamelon (<i>Meltric scabra</i> Naudin, Cucurbitaceae) is a climbing, herbaceous perennial plant with various culinary and medicinal uses. The current study aimed to develop novel autopolyploid genotypes of <i>M. scabra</i> through in vitro polyploidization and assess their...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/1/22 |
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| author | Eloy Fernández-Cusimamani Rohit Bharati Tereza Anna Javůrková Petra Škvorová Luboš Paznocht Zora Kotikova Lenka Kouřimská Matyáš Orsák |
| author_facet | Eloy Fernández-Cusimamani Rohit Bharati Tereza Anna Javůrková Petra Škvorová Luboš Paznocht Zora Kotikova Lenka Kouřimská Matyáš Orsák |
| author_sort | Eloy Fernández-Cusimamani |
| collection | DOAJ |
| description | Cucamelon (<i>Meltric scabra</i> Naudin, Cucurbitaceae) is a climbing, herbaceous perennial plant with various culinary and medicinal uses. The current study aimed to develop novel autopolyploid genotypes of <i>M. scabra</i> through in vitro polyploidization and assess their morphological and phytochemical characteristics. For polyploid induction, oryzalin was employed as an antimitotic agent, and it was applied at various concentrations (40, 60, and 80 μM) and durations (24 and 48 h). Flow cytometry analysis confirmed the successful induction of polyploids, with polyploidization efficiency ranging from 2.5% to 15%. From a total of 240 treated plants, a total of 20 autotetraploid plants were obtained. The obtained polyploid and control diploid genotypes were cultivated under greenhouse conditions. Further, the plants were transferred to field conditions, and the leaves, flowers, and fruits were harvested to be evaluated for the morphological, biochemical, and biological activity variations among the obtained genotypes. Morphological comparisons between diploid and autotetraploid plants revealed significant differences in flower characteristics, fruit attributes, and leaf morphology. Nutritional evaluation demonstrated enhancement of key parameters in the induced polyploids compared to the diploid plants, including glucose, fructose, carotenoid, polyphenol, and antioxidant contents, highlighting the potential impact of polyploidization on these traits. The results from this study highlight the potential of artificial chromosome doubling as an effective breeding strategy in <i>M. scabra</i> and related plant species. |
| first_indexed | 2024-03-08T10:49:02Z |
| format | Article |
| id | doaj.art-29c354b1d28940818e77c33f66da3922 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-08T10:49:02Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-29c354b1d28940818e77c33f66da39222024-01-26T16:49:40ZengMDPI AGHorticulturae2311-75242023-12-011012210.3390/horticulturae10010022Artificial Polyploidization Enhances Morphological, Physiological, and Biological Characteristics in <i>Melothria scabra</i> NaudinEloy Fernández-Cusimamani0Rohit Bharati1Tereza Anna Javůrková2Petra Škvorová3Luboš Paznocht4Zora Kotikova5Lenka Kouřimská6Matyáš Orsák7Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, 16500 Prague, Czech RepublicDepartment of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, 16500 Prague, Czech RepublicDepartment of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, 16500 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences, 16500 Prague, Czech RepublicDepartment of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 16500 Prague, Czech RepublicDepartment of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 16500 Prague, Czech RepublicDepartment of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences, 16500 Prague, Czech RepublicDepartment of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 16500 Prague, Czech RepublicCucamelon (<i>Meltric scabra</i> Naudin, Cucurbitaceae) is a climbing, herbaceous perennial plant with various culinary and medicinal uses. The current study aimed to develop novel autopolyploid genotypes of <i>M. scabra</i> through in vitro polyploidization and assess their morphological and phytochemical characteristics. For polyploid induction, oryzalin was employed as an antimitotic agent, and it was applied at various concentrations (40, 60, and 80 μM) and durations (24 and 48 h). Flow cytometry analysis confirmed the successful induction of polyploids, with polyploidization efficiency ranging from 2.5% to 15%. From a total of 240 treated plants, a total of 20 autotetraploid plants were obtained. The obtained polyploid and control diploid genotypes were cultivated under greenhouse conditions. Further, the plants were transferred to field conditions, and the leaves, flowers, and fruits were harvested to be evaluated for the morphological, biochemical, and biological activity variations among the obtained genotypes. Morphological comparisons between diploid and autotetraploid plants revealed significant differences in flower characteristics, fruit attributes, and leaf morphology. Nutritional evaluation demonstrated enhancement of key parameters in the induced polyploids compared to the diploid plants, including glucose, fructose, carotenoid, polyphenol, and antioxidant contents, highlighting the potential impact of polyploidization on these traits. The results from this study highlight the potential of artificial chromosome doubling as an effective breeding strategy in <i>M. scabra</i> and related plant species.https://www.mdpi.com/2311-7524/10/1/22chromosome doublingcucamelonoryzalinpolyploidizationplant breeding |
| spellingShingle | Eloy Fernández-Cusimamani Rohit Bharati Tereza Anna Javůrková Petra Škvorová Luboš Paznocht Zora Kotikova Lenka Kouřimská Matyáš Orsák Artificial Polyploidization Enhances Morphological, Physiological, and Biological Characteristics in <i>Melothria scabra</i> Naudin Horticulturae chromosome doubling cucamelon oryzalin polyploidization plant breeding |
| title | Artificial Polyploidization Enhances Morphological, Physiological, and Biological Characteristics in <i>Melothria scabra</i> Naudin |
| title_full | Artificial Polyploidization Enhances Morphological, Physiological, and Biological Characteristics in <i>Melothria scabra</i> Naudin |
| title_fullStr | Artificial Polyploidization Enhances Morphological, Physiological, and Biological Characteristics in <i>Melothria scabra</i> Naudin |
| title_full_unstemmed | Artificial Polyploidization Enhances Morphological, Physiological, and Biological Characteristics in <i>Melothria scabra</i> Naudin |
| title_short | Artificial Polyploidization Enhances Morphological, Physiological, and Biological Characteristics in <i>Melothria scabra</i> Naudin |
| title_sort | artificial polyploidization enhances morphological physiological and biological characteristics in i melothria scabra i naudin |
| topic | chromosome doubling cucamelon oryzalin polyploidization plant breeding |
| url | https://www.mdpi.com/2311-7524/10/1/22 |
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