Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia
Glycosides of Cistanche (GC) is a preparation used extensively for its neuroprotective effect against neurological diseases, but its mechanisms of action remains incompletely understood. Here, we established a bilateral common carotid artery occlusion model of vascular dementia in rats and injected...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Neural Regeneration Research |
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| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=7;spage=1152;epage=1158;aulast=Zhang |
| _version_ | 1818517049732759552 |
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| author | Yan-mei Zhang Wei Wu Wei Ma Fang Wang Jun Yuan |
| author_facet | Yan-mei Zhang Wei Wu Wei Ma Fang Wang Jun Yuan |
| author_sort | Yan-mei Zhang |
| collection | DOAJ |
| description | Glycosides of Cistanche (GC) is a preparation used extensively for its neuroprotective effect against neurological diseases, but its mechanisms of action remains incompletely understood. Here, we established a bilateral common carotid artery occlusion model of vascular dementia in rats and injected the model rats with a suspension of GC (10 mg/kg/day, intraperitoneally) for 14 consecutive days. Immunohistochemistry showed that GC significantly reduced p-tau and amyloid beta (Aβ) immunoreactivity in the hippocampus of the model rats. Proteomic analysis demonstrated upregulation of mitochondrial precursor protein and downregulation of keratin type II cytoskeletal 6A after GC treatment compared with model rats that had received saline. Western blot assay confirmed these findings. Our results suggest that the neuroprotective effect of GC in vascular dementia occurs via the promotion of neuronal cytoskeleton regeneration. |
| first_indexed | 2024-12-11T00:50:56Z |
| format | Article |
| id | doaj.art-29c7e1f337c14ab38a97f3b9ab88da1a |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-11T00:50:56Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-29c7e1f337c14ab38a97f3b9ab88da1a2022-12-22T01:26:38ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742017-01-011271152115810.4103/1673-5374.211196Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementiaYan-mei ZhangWei WuWei MaFang WangJun YuanGlycosides of Cistanche (GC) is a preparation used extensively for its neuroprotective effect against neurological diseases, but its mechanisms of action remains incompletely understood. Here, we established a bilateral common carotid artery occlusion model of vascular dementia in rats and injected the model rats with a suspension of GC (10 mg/kg/day, intraperitoneally) for 14 consecutive days. Immunohistochemistry showed that GC significantly reduced p-tau and amyloid beta (Aβ) immunoreactivity in the hippocampus of the model rats. Proteomic analysis demonstrated upregulation of mitochondrial precursor protein and downregulation of keratin type II cytoskeletal 6A after GC treatment compared with model rats that had received saline. Western blot assay confirmed these findings. Our results suggest that the neuroprotective effect of GC in vascular dementia occurs via the promotion of neuronal cytoskeleton regeneration.http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=7;spage=1152;epage=1158;aulast=Zhangnerve regeneration; vascular dementia; glycosides of Cistanche; mitochondrial precursor protein; keratin type II cytoskeletal 6A; proteomics; neuroprotection; neural regeneration |
| spellingShingle | Yan-mei Zhang Wei Wu Wei Ma Fang Wang Jun Yuan Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia Neural Regeneration Research nerve regeneration; vascular dementia; glycosides of Cistanche; mitochondrial precursor protein; keratin type II cytoskeletal 6A; proteomics; neuroprotection; neural regeneration |
| title | Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia |
| title_full | Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia |
| title_fullStr | Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia |
| title_full_unstemmed | Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia |
| title_short | Effect of glycosides of Cistanche on the expression of mitochondrial precursor protein and keratin type II cytoskeletal 6A in a rat model of vascular dementia |
| title_sort | effect of glycosides of cistanche on the expression of mitochondrial precursor protein and keratin type ii cytoskeletal 6a in a rat model of vascular dementia |
| topic | nerve regeneration; vascular dementia; glycosides of Cistanche; mitochondrial precursor protein; keratin type II cytoskeletal 6A; proteomics; neuroprotection; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2017;volume=12;issue=7;spage=1152;epage=1158;aulast=Zhang |
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