High cell density cultures produced by internal retention: application in continuous ethanol fermentation
Ethanol has provoked great interest due to its potential as an alternative fuel. Nevertheless, fermentation processes must be developed by increasing the low volumetric productivity achieved in conventional cultures (batch or continuous) to make this product become economically competitive. This can...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Spanish |
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Universidad Nacional de Colombia
2007-04-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/522 |
| _version_ | 1819295518360076288 |
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| author | Berta Carola Pérez Sonia Amparo Ospina Rubén Dario Godoy |
| author_facet | Berta Carola Pérez Sonia Amparo Ospina Rubén Dario Godoy |
| author_sort | Berta Carola Pérez |
| collection | DOAJ |
| description | Ethanol has provoked great interest due to its potential as an alternative fuel. Nevertheless, fermentation processes must be developed by increasing the low volumetric productivity achieved in conventional cultures (batch or continuous) to make this product become economically competitive. This can be achieved by using techniques leading to high cell concentration and reducing inhibition by the end-product. One of the frequently employed methods involves cell recycling. This work thus developed a membrane reactor incorporating a filtration module with 5 u,m stainless steel tubular units inside a 3L stirred jar fermenter for investigating its application in continuous ethanol production. The effects of cell concentration and transmembrane pressure difference on permeate flux were evaluated for testing the filtration module's performance. The internal cell retention system was operated in Saccharomyces cerevisiae continuous culture derived from sucrose, once fermentation conditions had been selected (30 °C, 1.25 -1.75 vvm, pH 4.5). Filter unit permeability was maintained by applying pulses of air. More than 97% of the grown cells were retained in the fermenter, reaching 51 g/L cell concentration and 8.51 g/L.h average ethanol productivity in culture with internal cell retention; this was twice that obtained in a conventional continuous culture.
Key words: Membrane reactor, Saccharomyces cerevisiae, alcoholic fermentation, cell recycling. |
| first_indexed | 2024-12-24T04:43:29Z |
| format | Article |
| id | doaj.art-29c9305c35874514b817190e445b215c |
| institution | Directory Open Access Journal |
| issn | 0123-3475 1909-8758 |
| language | Spanish |
| last_indexed | 2024-12-24T04:43:29Z |
| publishDate | 2007-04-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Biotecnología |
| spelling | doaj.art-29c9305c35874514b817190e445b215c2022-12-21T17:14:44ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582007-04-01622530482High cell density cultures produced by internal retention: application in continuous ethanol fermentationBerta Carola Pérez0Sonia Amparo Ospina1Rubén Dario Godoy2Ingeniera QuímicaQuímica farmaceuta, Ph. D.Ingeniero Químico, M. ScEthanol has provoked great interest due to its potential as an alternative fuel. Nevertheless, fermentation processes must be developed by increasing the low volumetric productivity achieved in conventional cultures (batch or continuous) to make this product become economically competitive. This can be achieved by using techniques leading to high cell concentration and reducing inhibition by the end-product. One of the frequently employed methods involves cell recycling. This work thus developed a membrane reactor incorporating a filtration module with 5 u,m stainless steel tubular units inside a 3L stirred jar fermenter for investigating its application in continuous ethanol production. The effects of cell concentration and transmembrane pressure difference on permeate flux were evaluated for testing the filtration module's performance. The internal cell retention system was operated in Saccharomyces cerevisiae continuous culture derived from sucrose, once fermentation conditions had been selected (30 °C, 1.25 -1.75 vvm, pH 4.5). Filter unit permeability was maintained by applying pulses of air. More than 97% of the grown cells were retained in the fermenter, reaching 51 g/L cell concentration and 8.51 g/L.h average ethanol productivity in culture with internal cell retention; this was twice that obtained in a conventional continuous culture. Key words: Membrane reactor, Saccharomyces cerevisiae, alcoholic fermentation, cell recycling.http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/522reactor de membranaSaccharomyces cerevisiaefermentación alcohólicarecirculación celularMembrane reactorSaccharomyces cerevisiaealcoholic fermentationcell recycling |
| spellingShingle | Berta Carola Pérez Sonia Amparo Ospina Rubén Dario Godoy High cell density cultures produced by internal retention: application in continuous ethanol fermentation Revista Colombiana de Biotecnología reactor de membrana Saccharomyces cerevisiae fermentación alcohólica recirculación celular Membrane reactor Saccharomyces cerevisiae alcoholic fermentation cell recycling |
| title | High cell density cultures produced by internal retention: application in continuous ethanol fermentation |
| title_full | High cell density cultures produced by internal retention: application in continuous ethanol fermentation |
| title_fullStr | High cell density cultures produced by internal retention: application in continuous ethanol fermentation |
| title_full_unstemmed | High cell density cultures produced by internal retention: application in continuous ethanol fermentation |
| title_short | High cell density cultures produced by internal retention: application in continuous ethanol fermentation |
| title_sort | high cell density cultures produced by internal retention application in continuous ethanol fermentation |
| topic | reactor de membrana Saccharomyces cerevisiae fermentación alcohólica recirculación celular Membrane reactor Saccharomyces cerevisiae alcoholic fermentation cell recycling |
| url | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/522 |
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