In Vitro Study of the Interaction of Gentamicin with Ceftriaxone and Azithromycin against <i>Neisseria gonorrhoeae</i> Using Agar Dilution Method
The susceptibility to gentamicin of <i>N. gonorrhoeae</i> isolates collected in three Chinese provinces and the correlation among the MICs of gentamicin, azithromycin, and ceftriaxone were investigated in this study. The effects of combinations from those three antibiotics were also in t...
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MDPI AG
2022-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/8/1083 |
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| author | Wenqi Xu Qian Zhou Jingwei Liu Yan Zhang Xiaoyu Zhu Bangyong Zhu Yueping Yin |
| author_facet | Wenqi Xu Qian Zhou Jingwei Liu Yan Zhang Xiaoyu Zhu Bangyong Zhu Yueping Yin |
| author_sort | Wenqi Xu |
| collection | DOAJ |
| description | The susceptibility to gentamicin of <i>N. gonorrhoeae</i> isolates collected in three Chinese provinces and the correlation among the MICs of gentamicin, azithromycin, and ceftriaxone were investigated in this study. The effects of combinations from those three antibiotics were also in the scope of this study to determine the efficacy of gentamicin as a combination therapeutic drug. The agar dilution method was used to measure the minimum inhibitory concentrations (MICs) of ceftriaxone, azithromycin and gentamicin on <i>N. gonorrhoeae</i> isolates. The synergy between these three antimicrobials were determined using the agar dilution checkerboard method. Subgroup studies were conducted to explore differences between azithromycin- and ceftriaxone-sensitive and resistant isolates. A total of 139 (36.60%) and 233 (61.30%) isolates demonstrated full susceptibility and intermediate susceptibility to gentamicin, respectively. The correlation analysis showed that the MICs of ceftriaxone and azithromycin weakly correlated with the value of gentamicin. The overall results of the three antibiotic combinations revealed indifferent effects. Combination therapy established a significant reduction on the MIC value. Most of the <i>N. gonorrhoeae</i> isolates tested in this study demonstrated a certain degree of susceptibility to gentamicin. Overall, antimicrobial combinations of gentamicin with ceftriaxone or azithromycin demonstrate indifferent effects. |
| first_indexed | 2024-03-09T04:46:38Z |
| format | Article |
| id | doaj.art-29cb79b83ac14f6c910dafbec5a89162 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T04:46:38Z |
| publishDate | 2022-08-01 |
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| series | Antibiotics |
| spelling | doaj.art-29cb79b83ac14f6c910dafbec5a891622023-12-03T13:14:46ZengMDPI AGAntibiotics2079-63822022-08-01118108310.3390/antibiotics11081083In Vitro Study of the Interaction of Gentamicin with Ceftriaxone and Azithromycin against <i>Neisseria gonorrhoeae</i> Using Agar Dilution MethodWenqi Xu0Qian Zhou1Jingwei Liu2Yan Zhang3Xiaoyu Zhu4Bangyong Zhu5Yueping Yin6Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, ChinaInstitute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, ChinaInstitute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, ChinaInstitute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, ChinaInstitute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, ChinaInstitute of Dermatology, Guangxi Autonomous Region, Nanning 530003, ChinaInstitute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, ChinaThe susceptibility to gentamicin of <i>N. gonorrhoeae</i> isolates collected in three Chinese provinces and the correlation among the MICs of gentamicin, azithromycin, and ceftriaxone were investigated in this study. The effects of combinations from those three antibiotics were also in the scope of this study to determine the efficacy of gentamicin as a combination therapeutic drug. The agar dilution method was used to measure the minimum inhibitory concentrations (MICs) of ceftriaxone, azithromycin and gentamicin on <i>N. gonorrhoeae</i> isolates. The synergy between these three antimicrobials were determined using the agar dilution checkerboard method. Subgroup studies were conducted to explore differences between azithromycin- and ceftriaxone-sensitive and resistant isolates. A total of 139 (36.60%) and 233 (61.30%) isolates demonstrated full susceptibility and intermediate susceptibility to gentamicin, respectively. The correlation analysis showed that the MICs of ceftriaxone and azithromycin weakly correlated with the value of gentamicin. The overall results of the three antibiotic combinations revealed indifferent effects. Combination therapy established a significant reduction on the MIC value. Most of the <i>N. gonorrhoeae</i> isolates tested in this study demonstrated a certain degree of susceptibility to gentamicin. Overall, antimicrobial combinations of gentamicin with ceftriaxone or azithromycin demonstrate indifferent effects.https://www.mdpi.com/2079-6382/11/8/1083<i>Neisseria gonorrhoeae</i>synergygentamicin |
| spellingShingle | Wenqi Xu Qian Zhou Jingwei Liu Yan Zhang Xiaoyu Zhu Bangyong Zhu Yueping Yin In Vitro Study of the Interaction of Gentamicin with Ceftriaxone and Azithromycin against <i>Neisseria gonorrhoeae</i> Using Agar Dilution Method Antibiotics <i>Neisseria gonorrhoeae</i> synergy gentamicin |
| title | In Vitro Study of the Interaction of Gentamicin with Ceftriaxone and Azithromycin against <i>Neisseria gonorrhoeae</i> Using Agar Dilution Method |
| title_full | In Vitro Study of the Interaction of Gentamicin with Ceftriaxone and Azithromycin against <i>Neisseria gonorrhoeae</i> Using Agar Dilution Method |
| title_fullStr | In Vitro Study of the Interaction of Gentamicin with Ceftriaxone and Azithromycin against <i>Neisseria gonorrhoeae</i> Using Agar Dilution Method |
| title_full_unstemmed | In Vitro Study of the Interaction of Gentamicin with Ceftriaxone and Azithromycin against <i>Neisseria gonorrhoeae</i> Using Agar Dilution Method |
| title_short | In Vitro Study of the Interaction of Gentamicin with Ceftriaxone and Azithromycin against <i>Neisseria gonorrhoeae</i> Using Agar Dilution Method |
| title_sort | in vitro study of the interaction of gentamicin with ceftriaxone and azithromycin against i neisseria gonorrhoeae i using agar dilution method |
| topic | <i>Neisseria gonorrhoeae</i> synergy gentamicin |
| url | https://www.mdpi.com/2079-6382/11/8/1083 |
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