Single-cell RNA-seq reveals developmental deficiencies in both the placentation and the decidualization in women with late-onset preeclampsia
Preeclampsia (PE) is a leading cause of maternal and fetal morbidity and mortality. Although increasing lines of evidence suggest that both the placenta and the decidua likely play roles in the pathogenesis of PE, the molecular mechanism of PE remains elusive partly because of the heterogeneity natu...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1142273/full |
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| author | Jing Yang Lili Gong Qiming Liu Huanqiang Zhao Zekun Wang Xiaotian Li Xiaotian Li Weidong Tian Weidong Tian Weidong Tian Qiongjie Zhou Qiongjie Zhou |
| author_facet | Jing Yang Lili Gong Qiming Liu Huanqiang Zhao Zekun Wang Xiaotian Li Xiaotian Li Weidong Tian Weidong Tian Weidong Tian Qiongjie Zhou Qiongjie Zhou |
| author_sort | Jing Yang |
| collection | DOAJ |
| description | Preeclampsia (PE) is a leading cause of maternal and fetal morbidity and mortality. Although increasing lines of evidence suggest that both the placenta and the decidua likely play roles in the pathogenesis of PE, the molecular mechanism of PE remains elusive partly because of the heterogeneity nature of the maternal-fetal interface. In this study, we perform single-cell RNA-seq on the placenta and the decidual from patients with late-onset PE (LOPE) and women in normal pregnancy. Analyses of single-cell transcriptomes reveal that in LOPE, there are likely a global development deficiency of trophoblasts with impaired invasion of extravillous trophoblasts (EVT) and increased maternal immune rejection and inflammation in the placenta, while there are likely insufficient decidualization of decidual stromal cells (DSC), increased inflammation, and suppressed regulatory functions of decidual immune cells. These findings improve our understanding of the molecular mechanisms of PE. |
| first_indexed | 2024-03-13T10:10:18Z |
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| id | doaj.art-29cc4173682b4a9eb334e75a7d592403 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-13T10:10:18Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-29cc4173682b4a9eb334e75a7d5924032023-05-22T04:26:43ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-05-011410.3389/fimmu.2023.11422731142273Single-cell RNA-seq reveals developmental deficiencies in both the placentation and the decidualization in women with late-onset preeclampsiaJing Yang0Lili Gong1Qiming Liu2Huanqiang Zhao3Zekun Wang4Xiaotian Li5Xiaotian Li6Weidong Tian7Weidong Tian8Weidong Tian9Qiongjie Zhou10Qiongjie Zhou11State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, Department of Computational Biology, School of Life Sciences, Fudan University, Shanghai, ChinaObstetrics and Gynaecology Hospital, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, Department of Computational Biology, School of Life Sciences, Fudan University, Shanghai, ChinaObstetrics and Gynaecology Hospital, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, Department of Computational Biology, School of Life Sciences, Fudan University, Shanghai, ChinaObstetrics and Gynaecology Hospital, Fudan University, Shanghai, ChinaObstetrics and Gynecology Hospital, Key Laboratory of Female Reproductive Endocrine-Related Diseases, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, Department of Computational Biology, School of Life Sciences, Fudan University, Shanghai, ChinaChildren’s Hospital of Fudan University, Shanghai, ChinaChildren’s Hospital of Shandong University, Jinan, Shandong, ChinaObstetrics and Gynaecology Hospital, Fudan University, Shanghai, ChinaObstetrics and Gynecology Hospital, Key Laboratory of Female Reproductive Endocrine-Related Diseases, Fudan University, Shanghai, ChinaPreeclampsia (PE) is a leading cause of maternal and fetal morbidity and mortality. Although increasing lines of evidence suggest that both the placenta and the decidua likely play roles in the pathogenesis of PE, the molecular mechanism of PE remains elusive partly because of the heterogeneity nature of the maternal-fetal interface. In this study, we perform single-cell RNA-seq on the placenta and the decidual from patients with late-onset PE (LOPE) and women in normal pregnancy. Analyses of single-cell transcriptomes reveal that in LOPE, there are likely a global development deficiency of trophoblasts with impaired invasion of extravillous trophoblasts (EVT) and increased maternal immune rejection and inflammation in the placenta, while there are likely insufficient decidualization of decidual stromal cells (DSC), increased inflammation, and suppressed regulatory functions of decidual immune cells. These findings improve our understanding of the molecular mechanisms of PE.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1142273/fullpreeclampsiasingle-cell RNA-seqplacentadeciduaepithelial-mesenchymal transition |
| spellingShingle | Jing Yang Lili Gong Qiming Liu Huanqiang Zhao Zekun Wang Xiaotian Li Xiaotian Li Weidong Tian Weidong Tian Weidong Tian Qiongjie Zhou Qiongjie Zhou Single-cell RNA-seq reveals developmental deficiencies in both the placentation and the decidualization in women with late-onset preeclampsia Frontiers in Immunology preeclampsia single-cell RNA-seq placenta decidua epithelial-mesenchymal transition |
| title | Single-cell RNA-seq reveals developmental deficiencies in both the placentation and the decidualization in women with late-onset preeclampsia |
| title_full | Single-cell RNA-seq reveals developmental deficiencies in both the placentation and the decidualization in women with late-onset preeclampsia |
| title_fullStr | Single-cell RNA-seq reveals developmental deficiencies in both the placentation and the decidualization in women with late-onset preeclampsia |
| title_full_unstemmed | Single-cell RNA-seq reveals developmental deficiencies in both the placentation and the decidualization in women with late-onset preeclampsia |
| title_short | Single-cell RNA-seq reveals developmental deficiencies in both the placentation and the decidualization in women with late-onset preeclampsia |
| title_sort | single cell rna seq reveals developmental deficiencies in both the placentation and the decidualization in women with late onset preeclampsia |
| topic | preeclampsia single-cell RNA-seq placenta decidua epithelial-mesenchymal transition |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1142273/full |
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