Molecular characterization of turnip yellows virus isolates from canola in Serbia
In November 2019, virus-like symptoms resembling those caused by turnip yellows virus (TuYV) were observed in many canola crops across Serbia. In order to identify their causal agent, a total of 206 samples were collected and analyzed for the presence of TuYV, cauliflower mosaic virus (CaMV) and tur...
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| Format: | Article |
| Language: | English |
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University of Kragujevac, Faculty of Agronomy, Cacak
2022-01-01
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| Series: | Acta Agriculturae Serbica |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/0354-9542/2022/0354-95422253031S.pdf |
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| author | Stanković Ivana Zečević Katarina Delibašić Goran Ivanović Lidija Milošević Dragana Marčić Milena Krstić Branka |
| author_facet | Stanković Ivana Zečević Katarina Delibašić Goran Ivanović Lidija Milošević Dragana Marčić Milena Krstić Branka |
| author_sort | Stanković Ivana |
| collection | DOAJ |
| description | In November 2019, virus-like symptoms resembling those caused by turnip yellows virus (TuYV) were observed in many canola crops across Serbia. In order to identify their causal agent, a total of 206 samples were collected and analyzed for the presence of TuYV, cauliflower mosaic virus (CaMV) and turnip mosaic virus (TuMV), using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was detected serologically in 91.75% of tested samples collected at 24 locations in all of seven inspected districts. None of the samples tested positive for TuMV and CaMV. Further molecular characterization based on the partial P0 gene sequences of seven selected ELISA-positive samples showed that Serbian TuYV isolates collected in 2019 shared low nucleotide diversity, and that they were closely related to previously identified Serbian cabbage and mustard isolates of TuYV. Phylogenetic analysis showed that TuYV isolates from Serbia were clustered within the TuYV/BrYV group. Moreover, nucleotide and amino acid sequence comparison of all TuYV isolates originating from Serbia, supported with a phylogenetic tree, indicated the existence of two virus subpopulations in Serbia. Further research should focus on determining the variability of TuYV population in Serbia, based on a whole-genome analysis that will contribute to a better understanding of the epidemiology of this pathogen, aiming at developing and implementing appropriate control measures. |
| first_indexed | 2024-12-10T09:32:35Z |
| format | Article |
| id | doaj.art-29cd1b2d283d461d9ba0b4423bfe2ca2 |
| institution | Directory Open Access Journal |
| issn | 0354-9542 2560-3140 |
| language | English |
| last_indexed | 2024-12-10T09:32:35Z |
| publishDate | 2022-01-01 |
| publisher | University of Kragujevac, Faculty of Agronomy, Cacak |
| record_format | Article |
| series | Acta Agriculturae Serbica |
| spelling | doaj.art-29cd1b2d283d461d9ba0b4423bfe2ca22022-12-22T01:54:17ZengUniversity of Kragujevac, Faculty of Agronomy, CacakActa Agriculturae Serbica0354-95422560-31402022-01-012753313710.5937/AASer2253031S0354-95422253031SMolecular characterization of turnip yellows virus isolates from canola in SerbiaStanković Ivana0https://orcid.org/0000-0003-3828-4718Zečević Katarina1Delibašić Goran2https://orcid.org/0000-0002-2024-1122Ivanović Lidija3Milošević Dragana4https://orcid.org/0000-0001-6392-9253Marčić Milena5Krstić Branka6University of Belgrade, Faculty of Agriculture, Institute of Phytomedicine, Department of Phytopathology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Agriculture, Institute of Phytomedicine, Department of Phytopathology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Agriculture, Institute of Phytomedicine, Department of Phytopathology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Agriculture, Institute of Phytomedicine, Department of Phytopathology, Belgrade, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaPlant Protection Forecasting and Warning Service, Novi Sad, SerbiaUniversity of Belgrade, Faculty of Agriculture, Institute of Phytomedicine, Department of Phytopathology, Belgrade, SerbiaIn November 2019, virus-like symptoms resembling those caused by turnip yellows virus (TuYV) were observed in many canola crops across Serbia. In order to identify their causal agent, a total of 206 samples were collected and analyzed for the presence of TuYV, cauliflower mosaic virus (CaMV) and turnip mosaic virus (TuMV), using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was detected serologically in 91.75% of tested samples collected at 24 locations in all of seven inspected districts. None of the samples tested positive for TuMV and CaMV. Further molecular characterization based on the partial P0 gene sequences of seven selected ELISA-positive samples showed that Serbian TuYV isolates collected in 2019 shared low nucleotide diversity, and that they were closely related to previously identified Serbian cabbage and mustard isolates of TuYV. Phylogenetic analysis showed that TuYV isolates from Serbia were clustered within the TuYV/BrYV group. Moreover, nucleotide and amino acid sequence comparison of all TuYV isolates originating from Serbia, supported with a phylogenetic tree, indicated the existence of two virus subpopulations in Serbia. Further research should focus on determining the variability of TuYV population in Serbia, based on a whole-genome analysis that will contribute to a better understanding of the epidemiology of this pathogen, aiming at developing and implementing appropriate control measures.https://scindeks-clanci.ceon.rs/data/pdf/0354-9542/2022/0354-95422253031S.pdfturnip yellows viruscanoladas-elisamolecular detectionphylogenetic analysis |
| spellingShingle | Stanković Ivana Zečević Katarina Delibašić Goran Ivanović Lidija Milošević Dragana Marčić Milena Krstić Branka Molecular characterization of turnip yellows virus isolates from canola in Serbia Acta Agriculturae Serbica turnip yellows virus canola das-elisa molecular detection phylogenetic analysis |
| title | Molecular characterization of turnip yellows virus isolates from canola in Serbia |
| title_full | Molecular characterization of turnip yellows virus isolates from canola in Serbia |
| title_fullStr | Molecular characterization of turnip yellows virus isolates from canola in Serbia |
| title_full_unstemmed | Molecular characterization of turnip yellows virus isolates from canola in Serbia |
| title_short | Molecular characterization of turnip yellows virus isolates from canola in Serbia |
| title_sort | molecular characterization of turnip yellows virus isolates from canola in serbia |
| topic | turnip yellows virus canola das-elisa molecular detection phylogenetic analysis |
| url | https://scindeks-clanci.ceon.rs/data/pdf/0354-9542/2022/0354-95422253031S.pdf |
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