Minimally invasive in vivo endoscopic monitoring of dextran sulfate sodium-induced murine colitis
Ulcerative colitis (UC) is a gastrointestinal, autoimmune disease that causes ulceration and inflammation of the colon with an incidence of 10 out of every 100,000 people in North America and Western Europe. Though the specific cause is unknown, several studies have demonstrated that inflammatory ce...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | MethodsX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016122001248 |
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| author | Elizabeth A. Bullard Ariel I. Mundo Shelby N. Bess Kathryn Miller Priest Timothy J. Muldoon |
| author_facet | Elizabeth A. Bullard Ariel I. Mundo Shelby N. Bess Kathryn Miller Priest Timothy J. Muldoon |
| author_sort | Elizabeth A. Bullard |
| collection | DOAJ |
| description | Ulcerative colitis (UC) is a gastrointestinal, autoimmune disease that causes ulceration and inflammation of the colon with an incidence of 10 out of every 100,000 people in North America and Western Europe. Though the specific cause is unknown, several studies have demonstrated that inflammatory cells as well as environmental variables, genetics, and lifestyle behaviors can play a role in the long-term inflammatory response. Researchers have commonly used immunohistochemistry, western blotting and gene sequencing to establish the cellular processes behind UC relapse and remission. However, because these destructive methods necessitate the removal of a sample, they can only be used on non-living tissues. The use of minimally invasive approaches to evaluate the in vivo, longitudinal effects of UC on the mucosa in the colon is gaining popularity among clinicians and researchers. We have created a dextran sulfate sodium-induced model of UC in C57 mice based on the work of Wirtz et al., and a minimally invasive imaging modality to explore the changes in mucosal tissue during “active” and “in remission” UC. Briefly, C57 mice were given dextran sulfate sodium (DSS) dissolved in water in 5-day cycles with a remission/recovery period of 10 days. After 7 days post-DSS treatment and 7 days post-recovery, mice were anesthetized and exploratory endoscopies were performed to assess the mucosal changes that occur during the “active” and “remission” periods of UC. Value of protocol: • Minimally invasive induction of ulcerative colitis in a murine mouse model. • Minimally invasive longitudinal monitoring of “active” and “in remission” ulcerative colitis. • Our endoscopic based imaging modality can be used to validate the induction of ulcerative colitis and the potential treatment response for pre-clinical trials. |
| first_indexed | 2024-04-12T02:18:43Z |
| format | Article |
| id | doaj.art-29ce2d69b9f941909d4aab787c6a69d0 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-04-12T02:18:43Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-29ce2d69b9f941909d4aab787c6a69d02022-12-22T03:52:10ZengElsevierMethodsX2215-01612022-01-019101744Minimally invasive in vivo endoscopic monitoring of dextran sulfate sodium-induced murine colitisElizabeth A. Bullard0Ariel I. Mundo1Shelby N. Bess2Kathryn Miller Priest3Timothy J. Muldoon4Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR, United StatesDepartment of Biomedical Engineering, University of Arkansas, Fayetteville, AR, United StatesDepartment of Biomedical Engineering, University of Arkansas, Fayetteville, AR, United StatesDepartment of Biomedical Engineering, University of Arkansas, Fayetteville, AR, United StatesCorresponding author.; Department of Biomedical Engineering, University of Arkansas, Fayetteville, AR, United StatesUlcerative colitis (UC) is a gastrointestinal, autoimmune disease that causes ulceration and inflammation of the colon with an incidence of 10 out of every 100,000 people in North America and Western Europe. Though the specific cause is unknown, several studies have demonstrated that inflammatory cells as well as environmental variables, genetics, and lifestyle behaviors can play a role in the long-term inflammatory response. Researchers have commonly used immunohistochemistry, western blotting and gene sequencing to establish the cellular processes behind UC relapse and remission. However, because these destructive methods necessitate the removal of a sample, they can only be used on non-living tissues. The use of minimally invasive approaches to evaluate the in vivo, longitudinal effects of UC on the mucosa in the colon is gaining popularity among clinicians and researchers. We have created a dextran sulfate sodium-induced model of UC in C57 mice based on the work of Wirtz et al., and a minimally invasive imaging modality to explore the changes in mucosal tissue during “active” and “in remission” UC. Briefly, C57 mice were given dextran sulfate sodium (DSS) dissolved in water in 5-day cycles with a remission/recovery period of 10 days. After 7 days post-DSS treatment and 7 days post-recovery, mice were anesthetized and exploratory endoscopies were performed to assess the mucosal changes that occur during the “active” and “remission” periods of UC. Value of protocol: • Minimally invasive induction of ulcerative colitis in a murine mouse model. • Minimally invasive longitudinal monitoring of “active” and “in remission” ulcerative colitis. • Our endoscopic based imaging modality can be used to validate the induction of ulcerative colitis and the potential treatment response for pre-clinical trials.http://www.sciencedirect.com/science/article/pii/S2215016122001248Dextran sulfate sodiumUlcerative colitisEndoscopy |
| spellingShingle | Elizabeth A. Bullard Ariel I. Mundo Shelby N. Bess Kathryn Miller Priest Timothy J. Muldoon Minimally invasive in vivo endoscopic monitoring of dextran sulfate sodium-induced murine colitis MethodsX Dextran sulfate sodium Ulcerative colitis Endoscopy |
| title | Minimally invasive in vivo endoscopic monitoring of dextran sulfate sodium-induced murine colitis |
| title_full | Minimally invasive in vivo endoscopic monitoring of dextran sulfate sodium-induced murine colitis |
| title_fullStr | Minimally invasive in vivo endoscopic monitoring of dextran sulfate sodium-induced murine colitis |
| title_full_unstemmed | Minimally invasive in vivo endoscopic monitoring of dextran sulfate sodium-induced murine colitis |
| title_short | Minimally invasive in vivo endoscopic monitoring of dextran sulfate sodium-induced murine colitis |
| title_sort | minimally invasive in vivo endoscopic monitoring of dextran sulfate sodium induced murine colitis |
| topic | Dextran sulfate sodium Ulcerative colitis Endoscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2215016122001248 |
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