A Novel 53-kDa Nodulin of the Symbiosome Membrane of Soybean Nodules, Controlled by Bradyrhizobium japonicum
A nodule-specific 53-kDa protein (GmNOD53b) of the symbiosome membrane from soybean was isolated and its LysC digestion products were microsequenced. cDNA clones of this novel nodulin, obtained from cDNA library screening with an RT-PCR (reverse-transcriptase polymerase chain reaction)-generated hyb...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1999-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.3.218 |
| _version_ | 1828739728712663040 |
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| author | Till Winzer Andrea Bairl Monica Linder Dietmar Linder Dietrich Werner Peter Müller |
| author_facet | Till Winzer Andrea Bairl Monica Linder Dietmar Linder Dietrich Werner Peter Müller |
| author_sort | Till Winzer |
| collection | DOAJ |
| description | A nodule-specific 53-kDa protein (GmNOD53b) of the symbiosome membrane from soybean was isolated and its LysC digestion products were microsequenced. cDNA clones of this novel nodulin, obtained from cDNA library screening with an RT-PCR (reverse-transcriptase polymerase chain reaction)-generated hybridization probe exhibited no homology to proteins identified so far. The expression of GmNOD53b coincides with the onset of nitrogen fixation. Therefore, it is a late nodulin. Among other changes, the GmNOD53b is significantly reduced in nodules infected with the Bradyrhizobium japonicum mutant 184 on the protein level as well as on the level of mRNA expression, compared with the wild-type infected nodules. The reduction of GmNOD53b mRNA is related to an inactivation of the sipF gene in B. japonicum 184, coding for a functionally active signal peptidase. |
| first_indexed | 2024-04-13T00:27:34Z |
| format | Article |
| id | doaj.art-29ce4fe6db1240fd9c722ecc4b7248f1 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:27:34Z |
| publishDate | 1999-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-29ce4fe6db1240fd9c722ecc4b7248f12022-12-22T03:10:33ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061999-03-0112321822610.1094/MPMI.1999.12.3.218A Novel 53-kDa Nodulin of the Symbiosome Membrane of Soybean Nodules, Controlled by Bradyrhizobium japonicumTill WinzerAndrea BairlMonica LinderDietmar LinderDietrich WernerPeter MüllerA nodule-specific 53-kDa protein (GmNOD53b) of the symbiosome membrane from soybean was isolated and its LysC digestion products were microsequenced. cDNA clones of this novel nodulin, obtained from cDNA library screening with an RT-PCR (reverse-transcriptase polymerase chain reaction)-generated hybridization probe exhibited no homology to proteins identified so far. The expression of GmNOD53b coincides with the onset of nitrogen fixation. Therefore, it is a late nodulin. Among other changes, the GmNOD53b is significantly reduced in nodules infected with the Bradyrhizobium japonicum mutant 184 on the protein level as well as on the level of mRNA expression, compared with the wild-type infected nodules. The reduction of GmNOD53b mRNA is related to an inactivation of the sipF gene in B. japonicum 184, coding for a functionally active signal peptidase.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.3.218bacteroids |
| spellingShingle | Till Winzer Andrea Bairl Monica Linder Dietmar Linder Dietrich Werner Peter Müller A Novel 53-kDa Nodulin of the Symbiosome Membrane of Soybean Nodules, Controlled by Bradyrhizobium japonicum Molecular Plant-Microbe Interactions bacteroids |
| title | A Novel 53-kDa Nodulin of the Symbiosome Membrane of Soybean Nodules, Controlled by Bradyrhizobium japonicum |
| title_full | A Novel 53-kDa Nodulin of the Symbiosome Membrane of Soybean Nodules, Controlled by Bradyrhizobium japonicum |
| title_fullStr | A Novel 53-kDa Nodulin of the Symbiosome Membrane of Soybean Nodules, Controlled by Bradyrhizobium japonicum |
| title_full_unstemmed | A Novel 53-kDa Nodulin of the Symbiosome Membrane of Soybean Nodules, Controlled by Bradyrhizobium japonicum |
| title_short | A Novel 53-kDa Nodulin of the Symbiosome Membrane of Soybean Nodules, Controlled by Bradyrhizobium japonicum |
| title_sort | novel 53 kda nodulin of the symbiosome membrane of soybean nodules controlled by bradyrhizobium japonicum |
| topic | bacteroids |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.3.218 |
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