Stress due to vaccination may induce re-activation of latent bovine herpesvirus-1 in cattle
This study evidences that, stress due to vaccination can cause re-activation and clinical outbreak of bovine herpesvirus-1 in latently infected cattle. Three to four days following mass-vaccination of cattle against Foot and Mouth Disease in Karnataka of India, symptoms of acute respiratory distress...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2019-09-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/93749 |
| _version_ | 1797896730878935040 |
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| author | B M CHANDRANAIK POOJA GOPIKUMAR AMITHA REENA GOMES POORVI REDDY POOJAPPA NANDINI M SHOBHA RANI M D VENKATESHA S M BYREGOWDA |
| author_facet | B M CHANDRANAIK POOJA GOPIKUMAR AMITHA REENA GOMES POORVI REDDY POOJAPPA NANDINI M SHOBHA RANI M D VENKATESHA S M BYREGOWDA |
| author_sort | B M CHANDRANAIK |
| collection | DOAJ |
| description | This study evidences that, stress due to vaccination can cause re-activation and clinical outbreak of bovine herpesvirus-1 in latently infected cattle. Three to four days following mass-vaccination of cattle against Foot and Mouth Disease in Karnataka of India, symptoms of acute respiratory distress, conjunctivitis and vulvo-vaginitis were observed in vaccinated cattle in many villages of the state. Nasal and ocular swabs were collected from 25 ailing cattle in six villages of Hassan district of Karnataka. Upon bacteriological analysis, the samples were found negative for Pasteurella multocida. The serum samples from ailing animals were positive for bovine herpesvirus- 1 (BoHV-1) antibodies by Indirect-ELISA. The swab samples were found for BoHV-1 by PCR targeting glycoprotein- C gene of BoHV-1. The swab samples when subjected to virus isolation in MDBK cells yielded characteristic CPE of bunch of grape like clustering of cells by fifth passage. PCR targeting conserved region on glycoprotein-C gene on DNA extracted from cell-culture supernatants showing CPE confirmed the presence of BoHV-1. Nucleotide sequencing of the PCR amplicon showed that the BoHV-1 isolated during this study shared 100% sequence identity with BoHV-1 isolates from India, Switzerland and Brazil. This study emphasizes that stress due to vaccination can cause re-activation and clinical outbreak of bovine herpesvirus-1 in latently infected cattle. |
| first_indexed | 2024-04-10T07:46:23Z |
| format | Article |
| id | doaj.art-29d2760bf6764c1bb4645300c53d1b77 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:46:23Z |
| publishDate | 2019-09-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-29d2760bf6764c1bb4645300c53d1b772023-02-23T10:31:45ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272019-09-0189910.56093/ijans.v89i9.93749Stress due to vaccination may induce re-activation of latent bovine herpesvirus-1 in cattleB M CHANDRANAIK0POOJA GOPIKUMAR1AMITHA REENA GOMES2POORVI REDDY3POOJAPPA NANDINI4M SHOBHA RANI5M D VENKATESHA6S M BYREGOWDA7Scientist, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka 560 024 IndiaMSc Scholar, Department of Microbiology, St Josephs College, Bengaluru.Scientist, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka 560 024 IndiaLaboratory Technician, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka 560 024 IndiaLaboratory Technician, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka 560 024 IndiaScientist, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka 560 024 IndiaScientist, Karnataka Veterinary Animal and Fisheries Sciences University, Bengaluru, Karnataka 560 024 IndiaDirector, Institute of Animal Health and Veterinary Biologicals, Hebbal, BengaluruThis study evidences that, stress due to vaccination can cause re-activation and clinical outbreak of bovine herpesvirus-1 in latently infected cattle. Three to four days following mass-vaccination of cattle against Foot and Mouth Disease in Karnataka of India, symptoms of acute respiratory distress, conjunctivitis and vulvo-vaginitis were observed in vaccinated cattle in many villages of the state. Nasal and ocular swabs were collected from 25 ailing cattle in six villages of Hassan district of Karnataka. Upon bacteriological analysis, the samples were found negative for Pasteurella multocida. The serum samples from ailing animals were positive for bovine herpesvirus- 1 (BoHV-1) antibodies by Indirect-ELISA. The swab samples were found for BoHV-1 by PCR targeting glycoprotein- C gene of BoHV-1. The swab samples when subjected to virus isolation in MDBK cells yielded characteristic CPE of bunch of grape like clustering of cells by fifth passage. PCR targeting conserved region on glycoprotein-C gene on DNA extracted from cell-culture supernatants showing CPE confirmed the presence of BoHV-1. Nucleotide sequencing of the PCR amplicon showed that the BoHV-1 isolated during this study shared 100% sequence identity with BoHV-1 isolates from India, Switzerland and Brazil. This study emphasizes that stress due to vaccination can cause re-activation and clinical outbreak of bovine herpesvirus-1 in latently infected cattle.https://epubs.icar.org.in/index.php/IJAnS/article/view/93749Bovine herpesvirus-1LatencyRe-activationVaccination stressVirus isolation |
| spellingShingle | B M CHANDRANAIK POOJA GOPIKUMAR AMITHA REENA GOMES POORVI REDDY POOJAPPA NANDINI M SHOBHA RANI M D VENKATESHA S M BYREGOWDA Stress due to vaccination may induce re-activation of latent bovine herpesvirus-1 in cattle Indian Journal of Animal Sciences Bovine herpesvirus-1 Latency Re-activation Vaccination stress Virus isolation |
| title | Stress due to vaccination may induce re-activation of latent bovine herpesvirus-1 in cattle |
| title_full | Stress due to vaccination may induce re-activation of latent bovine herpesvirus-1 in cattle |
| title_fullStr | Stress due to vaccination may induce re-activation of latent bovine herpesvirus-1 in cattle |
| title_full_unstemmed | Stress due to vaccination may induce re-activation of latent bovine herpesvirus-1 in cattle |
| title_short | Stress due to vaccination may induce re-activation of latent bovine herpesvirus-1 in cattle |
| title_sort | stress due to vaccination may induce re activation of latent bovine herpesvirus 1 in cattle |
| topic | Bovine herpesvirus-1 Latency Re-activation Vaccination stress Virus isolation |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/93749 |
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