Fluoxetin Upregulates Connexin 43 Expression in Astrocyte
Introduction: Recent studies have shown that astrocytes play major roles in normal and disease condition of the central nervous system including multiple sclerosis (MS). Molecular target therapy studies in MS have revealed that connexin-43 (Cx43) and Aquaporin-4 (AQP4) contents of astrocytes undergo...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2014-02-01
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| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-198&slc_lang=en&sid=1 |
| _version_ | 1797282744012636160 |
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| author | Hossein Mostafavi Mojtaba Khaksarian Mohammad Taghi Joghataei Gholamreza Hassanzadeh Masoud Soleimani Sanaz Eftekhari Mansooreh Soleimani Kazem Mousavizadeh Mahmoud Reza Hadjighassem |
| author_facet | Hossein Mostafavi Mojtaba Khaksarian Mohammad Taghi Joghataei Gholamreza Hassanzadeh Masoud Soleimani Sanaz Eftekhari Mansooreh Soleimani Kazem Mousavizadeh Mahmoud Reza Hadjighassem |
| author_sort | Hossein Mostafavi |
| collection | DOAJ |
| description | Introduction: Recent studies have shown that astrocytes play major roles in normal and disease condition of the central nervous system including multiple sclerosis (MS). Molecular target therapy studies in MS have revealed that connexin-43 (Cx43) and Aquaporin-4 (AQP4) contents of astrocytes undergo expression alteration. Fluoxetine had some effects in MS patients unrelated to its known antidepressant effects. Some of fluoxetine effects were attributed to its capability of cAMP signaling pathway stimulation. This study aimed to investigate possible acute effects of fluoxetine on Cx43 and AQP4 expression in astrocyte.
Methods: Astrocytoma cells were treated for 24 hours with fluoxetine (10 and 20 &mug/ml) with or without adenyl cyclase (AC) and protein kinase A (PKA) inhibition. Cx43 expression at both mRNA and protein levels and AQP4 expression at mRNA level were evaluated.
Results: Acquired results showed that fluoxetine with and without AC and PKA inhibition resulted in Cx43 up-regulation both in mRNA and protein levels, whereas AQP4 expression have not changed.
Discussion: In conclusion, data showed that fluoxetine alone and in the absence of serotonin acutely up-regulated Cx43 expression in astrocytes that can be assumed in molecular target therapy of MS patients. It seems that cAMP involvement in fluoxetine effects need more researches. |
| first_indexed | 2024-03-07T17:17:52Z |
| format | Article |
| id | doaj.art-29d690f3c075489aa5c4275ca4e30e37 |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:17:52Z |
| publishDate | 2014-02-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-29d690f3c075489aa5c4275ca4e30e372024-03-02T21:26:39ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422014-02-01517479Fluoxetin Upregulates Connexin 43 Expression in AstrocyteHossein Mostafavi0Mojtaba Khaksarian1Mohammad Taghi Joghataei2Gholamreza Hassanzadeh3Masoud Soleimani4Sanaz Eftekhari5Mansooreh Soleimani6Kazem Mousavizadeh7Mahmoud Reza Hadjighassem8 Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran. Division of Neuroscience, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Department of Hematology, Tarbiat Modares University, Tehran, Iran. Division of Neuroscience, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. Department of Hematology, Tarbiat Modares University, Tehran, Iran. Division of Neuroscience, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. Department of Neuroscience, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran. Introduction: Recent studies have shown that astrocytes play major roles in normal and disease condition of the central nervous system including multiple sclerosis (MS). Molecular target therapy studies in MS have revealed that connexin-43 (Cx43) and Aquaporin-4 (AQP4) contents of astrocytes undergo expression alteration. Fluoxetine had some effects in MS patients unrelated to its known antidepressant effects. Some of fluoxetine effects were attributed to its capability of cAMP signaling pathway stimulation. This study aimed to investigate possible acute effects of fluoxetine on Cx43 and AQP4 expression in astrocyte. Methods: Astrocytoma cells were treated for 24 hours with fluoxetine (10 and 20 &mug/ml) with or without adenyl cyclase (AC) and protein kinase A (PKA) inhibition. Cx43 expression at both mRNA and protein levels and AQP4 expression at mRNA level were evaluated. Results: Acquired results showed that fluoxetine with and without AC and PKA inhibition resulted in Cx43 up-regulation both in mRNA and protein levels, whereas AQP4 expression have not changed. Discussion: In conclusion, data showed that fluoxetine alone and in the absence of serotonin acutely up-regulated Cx43 expression in astrocytes that can be assumed in molecular target therapy of MS patients. It seems that cAMP involvement in fluoxetine effects need more researches.http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-198&slc_lang=en&sid=1Astrocyte Fluoxetine cAMP Connexin-43 Aquaporin-4. |
| spellingShingle | Hossein Mostafavi Mojtaba Khaksarian Mohammad Taghi Joghataei Gholamreza Hassanzadeh Masoud Soleimani Sanaz Eftekhari Mansooreh Soleimani Kazem Mousavizadeh Mahmoud Reza Hadjighassem Fluoxetin Upregulates Connexin 43 Expression in Astrocyte Basic and Clinical Neuroscience Astrocyte Fluoxetine cAMP Connexin-43 Aquaporin-4. |
| title | Fluoxetin Upregulates Connexin 43 Expression in Astrocyte |
| title_full | Fluoxetin Upregulates Connexin 43 Expression in Astrocyte |
| title_fullStr | Fluoxetin Upregulates Connexin 43 Expression in Astrocyte |
| title_full_unstemmed | Fluoxetin Upregulates Connexin 43 Expression in Astrocyte |
| title_short | Fluoxetin Upregulates Connexin 43 Expression in Astrocyte |
| title_sort | fluoxetin upregulates connexin 43 expression in astrocyte |
| topic | Astrocyte Fluoxetine cAMP Connexin-43 Aquaporin-4. |
| url | http://bcn.iums.ac.ir/browse.php?a_code=A-10-1-198&slc_lang=en&sid=1 |
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