Extracts of <i>Rheum palmatum</i> and <i>Aloe vera</i> Show Beneficial Properties for the Synergistic Improvement of Oral Wound Healing
Various local and systemic factors compromise oral wound healing and may lead to wound dehiscence, inflammation, or ulcers. Currently, there is a lack of topical therapeutical options. Thus, this study aimed to investigate the effect of <i>Aloe vera</i> (AV) and <i>Rheum palmatum&l...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/10/2060 |
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| author | Lena Katharina Müller-Heupt Nadine Wiesmann Sofia Schröder Yüksel Korkmaz Nina Vierengel Jonathan Groß Rolf Dahm James Deschner Till Opatz Juergen Brieger Bilal Al-Nawas Peer W. Kämmerer |
| author_facet | Lena Katharina Müller-Heupt Nadine Wiesmann Sofia Schröder Yüksel Korkmaz Nina Vierengel Jonathan Groß Rolf Dahm James Deschner Till Opatz Juergen Brieger Bilal Al-Nawas Peer W. Kämmerer |
| author_sort | Lena Katharina Müller-Heupt |
| collection | DOAJ |
| description | Various local and systemic factors compromise oral wound healing and may lead to wound dehiscence, inflammation, or ulcers. Currently, there is a lack of topical therapeutical options. Thus, this study aimed to investigate the effect of <i>Aloe vera</i> (AV) and <i>Rheum palmatum</i> root (RPR) on oral wound healing capacity in vitro. The effect of AV and RPR on human primary fibroblast viability and migration was studied by measuring metabolic activity and gap closure in a scratch assay. Furthermore, cell cycle distribution and cytoskeletal features were analyzed. Antimicrobial activity against the oral pathogen <i>Porphyromonas gingivalis</i> was evaluated by broth microdilution assay. AV and RPR increased fibroblast migration after single agent treatment. Synergistic effects of the plant extract combination were observed regarding cellular migration which were confirmed by calculation of the phenomenological combination index (pCI), whereas the cell cycle distribution was not influenced. Furthermore, the combination of AV and RPR showed synergistic antibacterial effects as determined by the fractional inhibitory concentration index. This study demonstrated that the combination of AV and RPR can promote the migration of human primary fibroblasts in vitro and exert antimicrobial efficacy against <i>P. gingivalis</i>, suggesting these compounds for the topical treatment of wound healing disorders. |
| first_indexed | 2024-03-09T19:35:20Z |
| format | Article |
| id | doaj.art-29d7f08cd5434be88ba13702deb530bb |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T19:35:20Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-29d7f08cd5434be88ba13702deb530bb2023-11-24T01:54:57ZengMDPI AGPharmaceutics1999-49232022-09-011410206010.3390/pharmaceutics14102060Extracts of <i>Rheum palmatum</i> and <i>Aloe vera</i> Show Beneficial Properties for the Synergistic Improvement of Oral Wound HealingLena Katharina Müller-Heupt0Nadine Wiesmann1Sofia Schröder2Yüksel Korkmaz3Nina Vierengel4Jonathan Groß5Rolf Dahm6James Deschner7Till Opatz8Juergen Brieger9Bilal Al-Nawas10Peer W. Kämmerer11Department of Oral- and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Oral- and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Oral- and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Periodontology and Operative Dentistry, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55128 Mainz, GermanyDepartment of Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55128 Mainz, GermanyBeratung für Informationssysteme und Systemintegration, Gärtnergasse 1, 55116 Mainz, GermanyDepartment of Periodontology and Operative Dentistry, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55128 Mainz, GermanyDepartment of Otorhinolaryngology, University Medical Center Mainz, Langenbeckstr. 1, 55131 Mainz, GermanyDepartment of Oral- and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyDepartment of Oral- and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131 Mainz, GermanyVarious local and systemic factors compromise oral wound healing and may lead to wound dehiscence, inflammation, or ulcers. Currently, there is a lack of topical therapeutical options. Thus, this study aimed to investigate the effect of <i>Aloe vera</i> (AV) and <i>Rheum palmatum</i> root (RPR) on oral wound healing capacity in vitro. The effect of AV and RPR on human primary fibroblast viability and migration was studied by measuring metabolic activity and gap closure in a scratch assay. Furthermore, cell cycle distribution and cytoskeletal features were analyzed. Antimicrobial activity against the oral pathogen <i>Porphyromonas gingivalis</i> was evaluated by broth microdilution assay. AV and RPR increased fibroblast migration after single agent treatment. Synergistic effects of the plant extract combination were observed regarding cellular migration which were confirmed by calculation of the phenomenological combination index (pCI), whereas the cell cycle distribution was not influenced. Furthermore, the combination of AV and RPR showed synergistic antibacterial effects as determined by the fractional inhibitory concentration index. This study demonstrated that the combination of AV and RPR can promote the migration of human primary fibroblasts in vitro and exert antimicrobial efficacy against <i>P. gingivalis</i>, suggesting these compounds for the topical treatment of wound healing disorders.https://www.mdpi.com/1999-4923/14/10/2060<i>Aloe vera</i>broth microdilutionfibroblastsphenomenological combination index<i>Porphyromonas gingivalis</i>promigratoy effect |
| spellingShingle | Lena Katharina Müller-Heupt Nadine Wiesmann Sofia Schröder Yüksel Korkmaz Nina Vierengel Jonathan Groß Rolf Dahm James Deschner Till Opatz Juergen Brieger Bilal Al-Nawas Peer W. Kämmerer Extracts of <i>Rheum palmatum</i> and <i>Aloe vera</i> Show Beneficial Properties for the Synergistic Improvement of Oral Wound Healing Pharmaceutics <i>Aloe vera</i> broth microdilution fibroblasts phenomenological combination index <i>Porphyromonas gingivalis</i> promigratoy effect |
| title | Extracts of <i>Rheum palmatum</i> and <i>Aloe vera</i> Show Beneficial Properties for the Synergistic Improvement of Oral Wound Healing |
| title_full | Extracts of <i>Rheum palmatum</i> and <i>Aloe vera</i> Show Beneficial Properties for the Synergistic Improvement of Oral Wound Healing |
| title_fullStr | Extracts of <i>Rheum palmatum</i> and <i>Aloe vera</i> Show Beneficial Properties for the Synergistic Improvement of Oral Wound Healing |
| title_full_unstemmed | Extracts of <i>Rheum palmatum</i> and <i>Aloe vera</i> Show Beneficial Properties for the Synergistic Improvement of Oral Wound Healing |
| title_short | Extracts of <i>Rheum palmatum</i> and <i>Aloe vera</i> Show Beneficial Properties for the Synergistic Improvement of Oral Wound Healing |
| title_sort | extracts of i rheum palmatum i and i aloe vera i show beneficial properties for the synergistic improvement of oral wound healing |
| topic | <i>Aloe vera</i> broth microdilution fibroblasts phenomenological combination index <i>Porphyromonas gingivalis</i> promigratoy effect |
| url | https://www.mdpi.com/1999-4923/14/10/2060 |
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