Physical Activity Prevents Cartilage Degradation: A Metabolomics Study Pinpoints the Involvement of Vitamin B6
Osteoarthritis (OA) is predominantly characterized by the progressive degradation of articular cartilage, the connective tissue produced by chondrocytes, due to an imbalance between anabolic and catabolic processes. In addition, physical activity (PA) is recognized as an important tool for counterac...
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MDPI AG
2019-11-01
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| Online Access: | https://www.mdpi.com/2073-4409/8/11/1374 |
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| author | Michela Deiana Giovanni Malerba Luca Dalle Carbonare Samuele Cheri Cristina Patuzzo Grygoriy Tsenov Lucas Moron Dalla Tor Antonio Mori Gianantonio Saviola Donato Zipeto Federico Schena Monica Mottes Maria Teresa Valenti |
| author_facet | Michela Deiana Giovanni Malerba Luca Dalle Carbonare Samuele Cheri Cristina Patuzzo Grygoriy Tsenov Lucas Moron Dalla Tor Antonio Mori Gianantonio Saviola Donato Zipeto Federico Schena Monica Mottes Maria Teresa Valenti |
| author_sort | Michela Deiana |
| collection | DOAJ |
| description | Osteoarthritis (OA) is predominantly characterized by the progressive degradation of articular cartilage, the connective tissue produced by chondrocytes, due to an imbalance between anabolic and catabolic processes. In addition, physical activity (PA) is recognized as an important tool for counteracting OA. To evaluate PA effects on the chondrocyte lineage, we analyzed the expression of SOX9, COL2A1, and COMP in circulating progenitor cells following a half marathon (HM) performance. Therefore, we studied in-depth the involvement of metabolites affecting chondrocyte lineage, and we compared the metabolomic profile associated with PA by analyzing runners’ sera before and after HM performance. Interestingly, this study highlighted that metabolites involved in vitamin B6 salvage, such as pyridoxal 5’-phosphate and pyridoxamine 5’-phosphate, were highly modulated. To evaluate the effects of vitamin B6 in cartilage cells, we treated differentiated mesenchymal stem cells and the SW1353 chondrosarcoma cell line with vitamin B6 in the presence of IL1β, the inflammatory cytokine involved in OA. Our study describes, for the first time, the modulation of the vitamin B6 salvage pathway following PA and suggests a protective role of PA in OA through modulation of this pathway. |
| first_indexed | 2024-03-12T10:30:26Z |
| format | Article |
| id | doaj.art-29d8efa372354f3ea16b005d822d3f58 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T10:30:26Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-29d8efa372354f3ea16b005d822d3f582023-09-02T09:13:36ZengMDPI AGCells2073-44092019-11-01811137410.3390/cells8111374cells8111374Physical Activity Prevents Cartilage Degradation: A Metabolomics Study Pinpoints the Involvement of Vitamin B6Michela Deiana0Giovanni Malerba1Luca Dalle Carbonare2Samuele Cheri3Cristina Patuzzo4Grygoriy Tsenov5Lucas Moron Dalla Tor6Antonio Mori7Gianantonio Saviola8Donato Zipeto9Federico Schena10Monica Mottes11Maria Teresa Valenti12Department of Medicine, Internal Medicine, Section D, University of Verona, I-37134 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, I-37134 Verona, ItalyDepartment of Medicine, Internal Medicine, Section D, University of Verona, I-37134 Verona, ItalyDepartment of Medicine, Internal Medicine, Section D, University of Verona, I-37134 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, I-37134 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, I-37134 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, I-37134 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, I-37134 Verona, ItalyIstituti Clinici Scientifici Maugeri IRCCS, Rheumatology and Rehabilitation Uniti of the Institute of Castel Goffredo, I-46042 Mantua, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, I-37134 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, I-37134 Verona, ItalyDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, I-37134 Verona, ItalyDepartment of Medicine, Internal Medicine, Section D, University of Verona, I-37134 Verona, ItalyOsteoarthritis (OA) is predominantly characterized by the progressive degradation of articular cartilage, the connective tissue produced by chondrocytes, due to an imbalance between anabolic and catabolic processes. In addition, physical activity (PA) is recognized as an important tool for counteracting OA. To evaluate PA effects on the chondrocyte lineage, we analyzed the expression of SOX9, COL2A1, and COMP in circulating progenitor cells following a half marathon (HM) performance. Therefore, we studied in-depth the involvement of metabolites affecting chondrocyte lineage, and we compared the metabolomic profile associated with PA by analyzing runners’ sera before and after HM performance. Interestingly, this study highlighted that metabolites involved in vitamin B6 salvage, such as pyridoxal 5’-phosphate and pyridoxamine 5’-phosphate, were highly modulated. To evaluate the effects of vitamin B6 in cartilage cells, we treated differentiated mesenchymal stem cells and the SW1353 chondrosarcoma cell line with vitamin B6 in the presence of IL1β, the inflammatory cytokine involved in OA. Our study describes, for the first time, the modulation of the vitamin B6 salvage pathway following PA and suggests a protective role of PA in OA through modulation of this pathway.https://www.mdpi.com/2073-4409/8/11/1374physical activitycartilageosteoarthritismetabolomicssox9vit.b6 |
| spellingShingle | Michela Deiana Giovanni Malerba Luca Dalle Carbonare Samuele Cheri Cristina Patuzzo Grygoriy Tsenov Lucas Moron Dalla Tor Antonio Mori Gianantonio Saviola Donato Zipeto Federico Schena Monica Mottes Maria Teresa Valenti Physical Activity Prevents Cartilage Degradation: A Metabolomics Study Pinpoints the Involvement of Vitamin B6 Cells physical activity cartilage osteoarthritis metabolomics sox9 vit.b6 |
| title | Physical Activity Prevents Cartilage Degradation: A Metabolomics Study Pinpoints the Involvement of Vitamin B6 |
| title_full | Physical Activity Prevents Cartilage Degradation: A Metabolomics Study Pinpoints the Involvement of Vitamin B6 |
| title_fullStr | Physical Activity Prevents Cartilage Degradation: A Metabolomics Study Pinpoints the Involvement of Vitamin B6 |
| title_full_unstemmed | Physical Activity Prevents Cartilage Degradation: A Metabolomics Study Pinpoints the Involvement of Vitamin B6 |
| title_short | Physical Activity Prevents Cartilage Degradation: A Metabolomics Study Pinpoints the Involvement of Vitamin B6 |
| title_sort | physical activity prevents cartilage degradation a metabolomics study pinpoints the involvement of vitamin b6 |
| topic | physical activity cartilage osteoarthritis metabolomics sox9 vit.b6 |
| url | https://www.mdpi.com/2073-4409/8/11/1374 |
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