Protein as chemical cue: non-nutritional growth enhancement by exogenous protein in Pseudomonas putida KT2440.
Research pertaining to microbe-microbe and microbe-plant interactions has been largely limited to small molecules like quorum sensing chemicals. However, a few recent reports have indicated the role of complex molecules like proteins and polysaccharides in microbial communication. Here we demonstrat...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4130607?pdf=render |
| _version_ | 1818063135828869120 |
|---|---|
| author | Hiren Joshi Rachna Dave Vayalam P Venugopalan |
| author_facet | Hiren Joshi Rachna Dave Vayalam P Venugopalan |
| author_sort | Hiren Joshi |
| collection | DOAJ |
| description | Research pertaining to microbe-microbe and microbe-plant interactions has been largely limited to small molecules like quorum sensing chemicals. However, a few recent reports have indicated the role of complex molecules like proteins and polysaccharides in microbial communication. Here we demonstrate that exogenous proteins present in culture media can considerably accelerate the growth of Pseudomonas putida KT2440, even when such proteins are not internalized by the cells. The growth enhancement is observed when the exogenous protein is not used as a source of carbon or nitrogen. The data show non-specific nature of the protein inducing growth; growth enhancement was observed irrespective of the protein type. It is shown that growth enhancement is mediated via increased siderophore secretion in response to the exogenous protein, leading to better iron uptake. We highlight the ecological significance of the observation and hypothesize that exogenous proteins serve as chemical cues in the case of P.putida and are perceived as indicator of the presence of competitors in the environment. It is argued that enhanced siderophore secretion in response to exogenous protein helps P.putida establish numerical superiority over competitors by way of enhanced iron assimilation and quicker utilization of aromatic substrates. |
| first_indexed | 2024-12-10T14:15:18Z |
| format | Article |
| id | doaj.art-29db1fbfbddc4ebb971b6ba34b9f5f03 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T14:15:18Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-29db1fbfbddc4ebb971b6ba34b9f5f032022-12-22T01:45:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10373010.1371/journal.pone.0103730Protein as chemical cue: non-nutritional growth enhancement by exogenous protein in Pseudomonas putida KT2440.Hiren JoshiRachna DaveVayalam P VenugopalanResearch pertaining to microbe-microbe and microbe-plant interactions has been largely limited to small molecules like quorum sensing chemicals. However, a few recent reports have indicated the role of complex molecules like proteins and polysaccharides in microbial communication. Here we demonstrate that exogenous proteins present in culture media can considerably accelerate the growth of Pseudomonas putida KT2440, even when such proteins are not internalized by the cells. The growth enhancement is observed when the exogenous protein is not used as a source of carbon or nitrogen. The data show non-specific nature of the protein inducing growth; growth enhancement was observed irrespective of the protein type. It is shown that growth enhancement is mediated via increased siderophore secretion in response to the exogenous protein, leading to better iron uptake. We highlight the ecological significance of the observation and hypothesize that exogenous proteins serve as chemical cues in the case of P.putida and are perceived as indicator of the presence of competitors in the environment. It is argued that enhanced siderophore secretion in response to exogenous protein helps P.putida establish numerical superiority over competitors by way of enhanced iron assimilation and quicker utilization of aromatic substrates.http://europepmc.org/articles/PMC4130607?pdf=render |
| spellingShingle | Hiren Joshi Rachna Dave Vayalam P Venugopalan Protein as chemical cue: non-nutritional growth enhancement by exogenous protein in Pseudomonas putida KT2440. PLoS ONE |
| title | Protein as chemical cue: non-nutritional growth enhancement by exogenous protein in Pseudomonas putida KT2440. |
| title_full | Protein as chemical cue: non-nutritional growth enhancement by exogenous protein in Pseudomonas putida KT2440. |
| title_fullStr | Protein as chemical cue: non-nutritional growth enhancement by exogenous protein in Pseudomonas putida KT2440. |
| title_full_unstemmed | Protein as chemical cue: non-nutritional growth enhancement by exogenous protein in Pseudomonas putida KT2440. |
| title_short | Protein as chemical cue: non-nutritional growth enhancement by exogenous protein in Pseudomonas putida KT2440. |
| title_sort | protein as chemical cue non nutritional growth enhancement by exogenous protein in pseudomonas putida kt2440 |
| url | http://europepmc.org/articles/PMC4130607?pdf=render |
| work_keys_str_mv | AT hirenjoshi proteinaschemicalcuenonnutritionalgrowthenhancementbyexogenousproteininpseudomonasputidakt2440 AT rachnadave proteinaschemicalcuenonnutritionalgrowthenhancementbyexogenousproteininpseudomonasputidakt2440 AT vayalampvenugopalan proteinaschemicalcuenonnutritionalgrowthenhancementbyexogenousproteininpseudomonasputidakt2440 |