Protective Effect of the Ethanol Extract from Hericium erinaceus Against Ethanol-Induced Gastric Ulcers
The fruiting bodies of Hericium erinaceus have been widely used for the treatment of dyspepsia, chronic gastric ulcers, and enervation. There remains a lack of data on the role of an ethanol extract from H. erinaceus (EEH) on ethanol-induced gastric ulcers. The ethanol-induced experimental gastr...
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| Format: | Article |
| Language: | English |
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Institute of Animal Reproduction and Food Research
2021-09-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
| Subjects: | |
| Online Access: | http://journal.pan.olsztyn.pl/Protective-Effect-of-the-Ethanol-Extract-from-Hericium-erinaceus-Against-Ethanol,141560,0,2.html |
| _version_ | 1818881178671775744 |
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| author | Guoying Lv Xiaoya Song Zuofang Zhang |
| author_facet | Guoying Lv Xiaoya Song Zuofang Zhang |
| author_sort | Guoying Lv |
| collection | DOAJ |
| description | The fruiting bodies of Hericium erinaceus have been widely used for the treatment of dyspepsia, chronic gastric ulcers, and enervation. There remains a lack of data on the role of an ethanol extract from H. erinaceus (EEH) on ethanol-induced gastric ulcers. The ethanol-induced experimental gastric injury model was used to evaluate the gastroprotective activity of extracts. Ultra-high performance liquid chromatography-triple quadrupole-time of flight tandem mass spectrometry (UPLC-Triple-TOF-MS) analysis was used to identify the possible compounds present in EEH. Transcriptome sequencing(RNA-seq) and bioinformatics analyses were conducted to reveal the characteristics and molecular mechanism underlying EEH’s protective effect of on gastric tissue injury. Administration of EEH at doses of 0.625, 1.25, and 2.5 g/kg body weight prior to ethanol ingestion dose-dependently inhibited gastric ulcers. EEH also significantly increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) content in the gastric tissue. Twelve compounds from EEH were identified including three diterpene compounds, two heteroterpene compounds, three isoindolinone compounds, one aromatic compound, N-(1-deoxy-D-fructos-1-yl)-L-valine, adenosine, and lumichrome. These compounds promote the inhibition of pathways involved in gastric ulcer formation. The RNA-seq results suggest that EEH indirectly protects the gastric tissue from injury by regulating the cell cycle and biological functions, up-regulating several signal molecules, or activating several proteasome functions. It was concluded that EEH represents a potential therapeutic option to reduce the risk of gastric ulceration. |
| first_indexed | 2024-12-19T14:57:44Z |
| format | Article |
| id | doaj.art-29ddc1b1b310468fbb228b2e86596613 |
| institution | Directory Open Access Journal |
| issn | 2083-6007 |
| language | English |
| last_indexed | 2024-12-19T14:57:44Z |
| publishDate | 2021-09-01 |
| publisher | Institute of Animal Reproduction and Food Research |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj.art-29ddc1b1b310468fbb228b2e865966132022-12-21T20:16:40ZengInstitute of Animal Reproduction and Food ResearchPolish Journal of Food and Nutrition Sciences2083-60072021-09-0171333334110.31883/pjfns/141560141560Protective Effect of the Ethanol Extract from Hericium erinaceus Against Ethanol-Induced Gastric UlcersGuoying Lv0Xiaoya Song1Zuofang Zhang2Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang 310021, ChinaInstitute of Fungi, Lishui Academy of Agricultural and Forestry Sciences, Lishui, Zhejiang 323000, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang 310021, ChinaThe fruiting bodies of Hericium erinaceus have been widely used for the treatment of dyspepsia, chronic gastric ulcers, and enervation. There remains a lack of data on the role of an ethanol extract from H. erinaceus (EEH) on ethanol-induced gastric ulcers. The ethanol-induced experimental gastric injury model was used to evaluate the gastroprotective activity of extracts. Ultra-high performance liquid chromatography-triple quadrupole-time of flight tandem mass spectrometry (UPLC-Triple-TOF-MS) analysis was used to identify the possible compounds present in EEH. Transcriptome sequencing(RNA-seq) and bioinformatics analyses were conducted to reveal the characteristics and molecular mechanism underlying EEH’s protective effect of on gastric tissue injury. Administration of EEH at doses of 0.625, 1.25, and 2.5 g/kg body weight prior to ethanol ingestion dose-dependently inhibited gastric ulcers. EEH also significantly increased superoxide dismutase (SOD) activity and decreased malondialdehyde (MDA) content in the gastric tissue. Twelve compounds from EEH were identified including three diterpene compounds, two heteroterpene compounds, three isoindolinone compounds, one aromatic compound, N-(1-deoxy-D-fructos-1-yl)-L-valine, adenosine, and lumichrome. These compounds promote the inhibition of pathways involved in gastric ulcer formation. The RNA-seq results suggest that EEH indirectly protects the gastric tissue from injury by regulating the cell cycle and biological functions, up-regulating several signal molecules, or activating several proteasome functions. It was concluded that EEH represents a potential therapeutic option to reduce the risk of gastric ulceration.http://journal.pan.olsztyn.pl/Protective-Effect-of-the-Ethanol-Extract-from-Hericium-erinaceus-Against-Ethanol,141560,0,2.htmlmushroom ethanol extractgastroprotective activityhericium erinaceusbioinformatics analysis |
| spellingShingle | Guoying Lv Xiaoya Song Zuofang Zhang Protective Effect of the Ethanol Extract from Hericium erinaceus Against Ethanol-Induced Gastric Ulcers Polish Journal of Food and Nutrition Sciences mushroom ethanol extract gastroprotective activity hericium erinaceus bioinformatics analysis |
| title | Protective Effect of the Ethanol Extract from Hericium erinaceus Against Ethanol-Induced Gastric Ulcers |
| title_full | Protective Effect of the Ethanol Extract from Hericium erinaceus Against Ethanol-Induced Gastric Ulcers |
| title_fullStr | Protective Effect of the Ethanol Extract from Hericium erinaceus Against Ethanol-Induced Gastric Ulcers |
| title_full_unstemmed | Protective Effect of the Ethanol Extract from Hericium erinaceus Against Ethanol-Induced Gastric Ulcers |
| title_short | Protective Effect of the Ethanol Extract from Hericium erinaceus Against Ethanol-Induced Gastric Ulcers |
| title_sort | protective effect of the ethanol extract from hericium erinaceus against ethanol induced gastric ulcers |
| topic | mushroom ethanol extract gastroprotective activity hericium erinaceus bioinformatics analysis |
| url | http://journal.pan.olsztyn.pl/Protective-Effect-of-the-Ethanol-Extract-from-Hericium-erinaceus-Against-Ethanol,141560,0,2.html |
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