An ultrasensitive electrochemical immunosensor for CA72-4 based on a signal amplification strategy of MoS2 nanoflower-supported Au nanoparticles
Accurate detection of cancer antigen 72-4 (CA72-4), a tumor-associated glycoprotein, is of great significance for gastric cancer diagnosis and immunotherapy monitoring. Modification of noble metal nanoparticles on transition metal dichalcogenides can significantly enhance functions, such as electron...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
|
| Series: | Journal of Saudi Chemical Society |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319610323000169 |
| _version_ | 1797861135085469696 |
|---|---|
| author | Qinghua Yan Ruixue Wu Hongli Chen Wenbin Nan |
| author_facet | Qinghua Yan Ruixue Wu Hongli Chen Wenbin Nan |
| author_sort | Qinghua Yan |
| collection | DOAJ |
| description | Accurate detection of cancer antigen 72-4 (CA72-4), a tumor-associated glycoprotein, is of great significance for gastric cancer diagnosis and immunotherapy monitoring. Modification of noble metal nanoparticles on transition metal dichalcogenides can significantly enhance functions, such as electron transport. Molybdenum disulfide gold nanoparticles nanocomposites (MoS2-Au NPs) were prepared in this study and a series of characterization studies were carried out. In addition, a label-free, highly sensitive electrochemical immunosensor molybdenum disulfide -Au nanoparticles/Glassy carbon electrode (MoS2-Au NPs/GCE) was also prepared and used for the detection of CA72-4. The electrochemical performance of the immunosensor was characterized by electrochemical techniques, such as cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The results indicated that better MoS2-Au NPs nanomaterials have been synthesized, and the prepared electrochemical immunosensor, MoS2-Au NPs/GCE, showed excellent electrochemical performance. The sensor exhibited high detection sensitivity under optimal conditions, including an incubation time of 30 min, an incubation temperature of 25 °C, and a pH of 7.0. The electrochemical immunosensor also had a low detection limit of 2.0 × 10−5 U/mL (S/N = 3) in a concentration range of 0.001–200 U/mL, with good selectivity, stability, and repeatability. In conclusion, this study provided a theoretical basis for the highly sensitive detection of tumor markers in clinical biological samples. |
| first_indexed | 2024-04-09T21:57:11Z |
| format | Article |
| id | doaj.art-29e1cd74cafd423fa7b01bd4dc2fb7da |
| institution | Directory Open Access Journal |
| issn | 1319-6103 |
| language | English |
| last_indexed | 2024-04-09T21:57:11Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Saudi Chemical Society |
| spelling | doaj.art-29e1cd74cafd423fa7b01bd4dc2fb7da2023-03-24T04:22:07ZengElsevierJournal of Saudi Chemical Society1319-61032023-03-01272101612An ultrasensitive electrochemical immunosensor for CA72-4 based on a signal amplification strategy of MoS2 nanoflower-supported Au nanoparticlesQinghua Yan0Ruixue Wu1Hongli Chen2Wenbin Nan3The Key Laboratory of Biomedical Material, School of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, ChinaThe Key Laboratory of Biomedical Material, School of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, ChinaThe Key Laboratory of Biomedical Material, School of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, ChinaCorresponding author.; The Key Laboratory of Biomedical Material, School of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, ChinaAccurate detection of cancer antigen 72-4 (CA72-4), a tumor-associated glycoprotein, is of great significance for gastric cancer diagnosis and immunotherapy monitoring. Modification of noble metal nanoparticles on transition metal dichalcogenides can significantly enhance functions, such as electron transport. Molybdenum disulfide gold nanoparticles nanocomposites (MoS2-Au NPs) were prepared in this study and a series of characterization studies were carried out. In addition, a label-free, highly sensitive electrochemical immunosensor molybdenum disulfide -Au nanoparticles/Glassy carbon electrode (MoS2-Au NPs/GCE) was also prepared and used for the detection of CA72-4. The electrochemical performance of the immunosensor was characterized by electrochemical techniques, such as cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The results indicated that better MoS2-Au NPs nanomaterials have been synthesized, and the prepared electrochemical immunosensor, MoS2-Au NPs/GCE, showed excellent electrochemical performance. The sensor exhibited high detection sensitivity under optimal conditions, including an incubation time of 30 min, an incubation temperature of 25 °C, and a pH of 7.0. The electrochemical immunosensor also had a low detection limit of 2.0 × 10−5 U/mL (S/N = 3) in a concentration range of 0.001–200 U/mL, with good selectivity, stability, and repeatability. In conclusion, this study provided a theoretical basis for the highly sensitive detection of tumor markers in clinical biological samples.http://www.sciencedirect.com/science/article/pii/S1319610323000169Electrochemical immunosensorCA72-4MoS2-Au NPs composites |
| spellingShingle | Qinghua Yan Ruixue Wu Hongli Chen Wenbin Nan An ultrasensitive electrochemical immunosensor for CA72-4 based on a signal amplification strategy of MoS2 nanoflower-supported Au nanoparticles Journal of Saudi Chemical Society Electrochemical immunosensor CA72-4 MoS2-Au NPs composites |
| title | An ultrasensitive electrochemical immunosensor for CA72-4 based on a signal amplification strategy of MoS2 nanoflower-supported Au nanoparticles |
| title_full | An ultrasensitive electrochemical immunosensor for CA72-4 based on a signal amplification strategy of MoS2 nanoflower-supported Au nanoparticles |
| title_fullStr | An ultrasensitive electrochemical immunosensor for CA72-4 based on a signal amplification strategy of MoS2 nanoflower-supported Au nanoparticles |
| title_full_unstemmed | An ultrasensitive electrochemical immunosensor for CA72-4 based on a signal amplification strategy of MoS2 nanoflower-supported Au nanoparticles |
| title_short | An ultrasensitive electrochemical immunosensor for CA72-4 based on a signal amplification strategy of MoS2 nanoflower-supported Au nanoparticles |
| title_sort | ultrasensitive electrochemical immunosensor for ca72 4 based on a signal amplification strategy of mos2 nanoflower supported au nanoparticles |
| topic | Electrochemical immunosensor CA72-4 MoS2-Au NPs composites |
| url | http://www.sciencedirect.com/science/article/pii/S1319610323000169 |
| work_keys_str_mv | AT qinghuayan anultrasensitiveelectrochemicalimmunosensorforca724basedonasignalamplificationstrategyofmos2nanoflowersupportedaunanoparticles AT ruixuewu anultrasensitiveelectrochemicalimmunosensorforca724basedonasignalamplificationstrategyofmos2nanoflowersupportedaunanoparticles AT honglichen anultrasensitiveelectrochemicalimmunosensorforca724basedonasignalamplificationstrategyofmos2nanoflowersupportedaunanoparticles AT wenbinnan anultrasensitiveelectrochemicalimmunosensorforca724basedonasignalamplificationstrategyofmos2nanoflowersupportedaunanoparticles AT qinghuayan ultrasensitiveelectrochemicalimmunosensorforca724basedonasignalamplificationstrategyofmos2nanoflowersupportedaunanoparticles AT ruixuewu ultrasensitiveelectrochemicalimmunosensorforca724basedonasignalamplificationstrategyofmos2nanoflowersupportedaunanoparticles AT honglichen ultrasensitiveelectrochemicalimmunosensorforca724basedonasignalamplificationstrategyofmos2nanoflowersupportedaunanoparticles AT wenbinnan ultrasensitiveelectrochemicalimmunosensorforca724basedonasignalamplificationstrategyofmos2nanoflowersupportedaunanoparticles |