Expression and localization of absent in melanoma 2 in the injured spinal cord
In traumatic brain injury, absent in melanoma 2 (AIM2) has been demonstrated to be involved in pyroptotic neuronal cell death. Although the pathophysiological mechanism of spinal cord injury is similar to that of brain injury, the expression and cellular localization of AIM2 after spinal cord injury...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=3;spage=542;epage=552;aulast=Wang |
| _version_ | 1818117723755905024 |
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| author | Sai-Nan Wang Xue-Yan Guo Jie Tang Shu-Qin Ding Lin Shen Rui Wang Shan-Feng Ma Jian-Guo Hu He-Zuo Lü |
| author_facet | Sai-Nan Wang Xue-Yan Guo Jie Tang Shu-Qin Ding Lin Shen Rui Wang Shan-Feng Ma Jian-Guo Hu He-Zuo Lü |
| author_sort | Sai-Nan Wang |
| collection | DOAJ |
| description | In traumatic brain injury, absent in melanoma 2 (AIM2) has been demonstrated to be involved in pyroptotic neuronal cell death. Although the pathophysiological mechanism of spinal cord injury is similar to that of brain injury, the expression and cellular localization of AIM2 after spinal cord injury is still not very clear. In the present study, we used a rat model of T9 spinal cord contusive injury, produced using the weight drop method. The rats were randomly divided into 1-hour, 6-hour, 1-day, 3-day and 6-day (post-injury time points) groups. Sham-operated rats only received laminectomy at T9 without contusive injury. Western blot assay revealed that the expression levels of AIM2 were not significantly different among the 1-hour, 6-hour and 1-day groups. The expression levels of AIM2 were markedly higher in the 1-hour, 6-hour and 1-day groups compared with the sham, 3-day and 7-day groups. Double immunofluorescence staining demonstrated that AIM2 was expressed by NeuN+ (neurons), GFAP+ (astrocytes), CNPase+ (oligodendrocytes) and CD11b+ (microglia) cells in the sham-operated spinal cord. In rats with spinal cord injury, AIM2 was also found in CD45+ (leukocytes) and CD68+ (activated microglia/macrophages) cells in the spinal cord at all time points. These findings indicate that AIM2 is mainly expressed in neurons, astrocytes, microglia and oligodendrocytes in the normal spinal cord, and that after spinal cord injury, its expression increases because of the infiltration of leukocytes and the activation of astrocytes and microglia/macrophages. |
| first_indexed | 2024-12-11T04:42:57Z |
| format | Article |
| id | doaj.art-29e2d5ba6b924690b4f7017a6a07c61f |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-11T04:42:57Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-29e2d5ba6b924690b4f7017a6a07c61f2022-12-22T01:20:34ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742019-01-0114354255210.4103/1673-5374.245481Expression and localization of absent in melanoma 2 in the injured spinal cordSai-Nan WangXue-Yan GuoJie TangShu-Qin DingLin ShenRui WangShan-Feng MaJian-Guo HuHe-Zuo LüIn traumatic brain injury, absent in melanoma 2 (AIM2) has been demonstrated to be involved in pyroptotic neuronal cell death. Although the pathophysiological mechanism of spinal cord injury is similar to that of brain injury, the expression and cellular localization of AIM2 after spinal cord injury is still not very clear. In the present study, we used a rat model of T9 spinal cord contusive injury, produced using the weight drop method. The rats were randomly divided into 1-hour, 6-hour, 1-day, 3-day and 6-day (post-injury time points) groups. Sham-operated rats only received laminectomy at T9 without contusive injury. Western blot assay revealed that the expression levels of AIM2 were not significantly different among the 1-hour, 6-hour and 1-day groups. The expression levels of AIM2 were markedly higher in the 1-hour, 6-hour and 1-day groups compared with the sham, 3-day and 7-day groups. Double immunofluorescence staining demonstrated that AIM2 was expressed by NeuN+ (neurons), GFAP+ (astrocytes), CNPase+ (oligodendrocytes) and CD11b+ (microglia) cells in the sham-operated spinal cord. In rats with spinal cord injury, AIM2 was also found in CD45+ (leukocytes) and CD68+ (activated microglia/macrophages) cells in the spinal cord at all time points. These findings indicate that AIM2 is mainly expressed in neurons, astrocytes, microglia and oligodendrocytes in the normal spinal cord, and that after spinal cord injury, its expression increases because of the infiltration of leukocytes and the activation of astrocytes and microglia/macrophages.http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=3;spage=542;epage=552;aulast=Wangnerve regeneration; spinal cord injury; absent in melanoma 2; spatio-temporal expression; neurons; astrocytes; oligodendrocytes; infiltrated leukocytes; activated microglia; western blot assay; immunohistochemistry; neural regeneration |
| spellingShingle | Sai-Nan Wang Xue-Yan Guo Jie Tang Shu-Qin Ding Lin Shen Rui Wang Shan-Feng Ma Jian-Guo Hu He-Zuo Lü Expression and localization of absent in melanoma 2 in the injured spinal cord Neural Regeneration Research nerve regeneration; spinal cord injury; absent in melanoma 2; spatio-temporal expression; neurons; astrocytes; oligodendrocytes; infiltrated leukocytes; activated microglia; western blot assay; immunohistochemistry; neural regeneration |
| title | Expression and localization of absent in melanoma 2 in the injured spinal cord |
| title_full | Expression and localization of absent in melanoma 2 in the injured spinal cord |
| title_fullStr | Expression and localization of absent in melanoma 2 in the injured spinal cord |
| title_full_unstemmed | Expression and localization of absent in melanoma 2 in the injured spinal cord |
| title_short | Expression and localization of absent in melanoma 2 in the injured spinal cord |
| title_sort | expression and localization of absent in melanoma 2 in the injured spinal cord |
| topic | nerve regeneration; spinal cord injury; absent in melanoma 2; spatio-temporal expression; neurons; astrocytes; oligodendrocytes; infiltrated leukocytes; activated microglia; western blot assay; immunohistochemistry; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=3;spage=542;epage=552;aulast=Wang |
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