Cost‐Effective and Robust Multispectral Light‐Emitting Diode Device for the Readout of Plasmonic Microarray Sensors
Localized surface plasmon resonance (LSPR) is a phenomenon known for more than 100 years, which arises from the interaction of light with metallic nanoparticles. In recent years, the field of LSPR sensing has become increasingly important in bioanalytics. Herein, a simple and robust device setup to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-02-01
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| Series: | Advanced Photonics Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/adpr.202200252 |
| _version_ | 1828014425368428544 |
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| author | Stephan Kastner Matthias Urban Anne-Kathrin Dietel Andrea Csáki Wolfgang Fritzsche |
| author_facet | Stephan Kastner Matthias Urban Anne-Kathrin Dietel Andrea Csáki Wolfgang Fritzsche |
| author_sort | Stephan Kastner |
| collection | DOAJ |
| description | Localized surface plasmon resonance (LSPR) is a phenomenon known for more than 100 years, which arises from the interaction of light with metallic nanoparticles. In recent years, the field of LSPR sensing has become increasingly important in bioanalytics. Herein, a simple and robust device setup to perform time‐resolved LSPR measurements with inexpensive array sensor chips is presented. For this purpose, gold nanoparticles are spotted onto glass substrates under different conditions (droplet size/number, temperature, and humidity) to achieve an optimal signal‐to‐noise ratio. To verify the setup and the spotted sensor chips, bulk sensitivity measurements with solutions of varying refractive index and surface sensitivity measurements with layer‐by‐layer (LbL) deposition are performed. It is shown that slower drying minimizes the edge effects of ring‐like deposits (coffee ring effect) and that the spots with higher particle densities are more suitable for sensor applications. In general, the use of six light‐emitting diode (LEDs) enables a simple centroid calculation as well as an evaluation via individual LED intensities. The presented cost‐effective system allows parallel reading of more than 100 spots in a label‐free platform, and together with the optimized low‐cost sensors, it provides an interesting alternative for the development of future on‐site diagnostics. |
| first_indexed | 2024-04-10T10:02:01Z |
| format | Article |
| id | doaj.art-29e48b5eb5e94053828566b5710e64bd |
| institution | Directory Open Access Journal |
| issn | 2699-9293 |
| language | English |
| last_indexed | 2024-04-10T10:02:01Z |
| publishDate | 2023-02-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Photonics Research |
| spelling | doaj.art-29e48b5eb5e94053828566b5710e64bd2023-02-16T05:49:06ZengWiley-VCHAdvanced Photonics Research2699-92932023-02-0142n/an/a10.1002/adpr.202200252Cost‐Effective and Robust Multispectral Light‐Emitting Diode Device for the Readout of Plasmonic Microarray SensorsStephan Kastner0Matthias Urban1Anne-Kathrin Dietel2Andrea Csáki3Wolfgang Fritzsche4Department Nanobiophotonics Leibniz Institute of Photonic Technology (Leibniz-IPHT) Albert-Einstein-Strasse 9 07745 Jena GermanyDepartment Nanobiophotonics Leibniz Institute of Photonic Technology (Leibniz-IPHT) Albert-Einstein-Strasse 9 07745 Jena GermanyDepartment Nanobiophotonics Leibniz Institute of Photonic Technology (Leibniz-IPHT) Albert-Einstein-Strasse 9 07745 Jena GermanyDepartment Nanobiophotonics Leibniz Institute of Photonic Technology (Leibniz-IPHT) Albert-Einstein-Strasse 9 07745 Jena GermanyDepartment Nanobiophotonics Leibniz Institute of Photonic Technology (Leibniz-IPHT) Albert-Einstein-Strasse 9 07745 Jena GermanyLocalized surface plasmon resonance (LSPR) is a phenomenon known for more than 100 years, which arises from the interaction of light with metallic nanoparticles. In recent years, the field of LSPR sensing has become increasingly important in bioanalytics. Herein, a simple and robust device setup to perform time‐resolved LSPR measurements with inexpensive array sensor chips is presented. For this purpose, gold nanoparticles are spotted onto glass substrates under different conditions (droplet size/number, temperature, and humidity) to achieve an optimal signal‐to‐noise ratio. To verify the setup and the spotted sensor chips, bulk sensitivity measurements with solutions of varying refractive index and surface sensitivity measurements with layer‐by‐layer (LbL) deposition are performed. It is shown that slower drying minimizes the edge effects of ring‐like deposits (coffee ring effect) and that the spots with higher particle densities are more suitable for sensor applications. In general, the use of six light‐emitting diode (LEDs) enables a simple centroid calculation as well as an evaluation via individual LED intensities. The presented cost‐effective system allows parallel reading of more than 100 spots in a label‐free platform, and together with the optimized low‐cost sensors, it provides an interesting alternative for the development of future on‐site diagnostics.https://doi.org/10.1002/adpr.202200252localized surface plasmon resonance sensorsmicrofluidic systemsmultispectral light-emitting diode readoutnanoparticle spottingplasmonic microarrays |
| spellingShingle | Stephan Kastner Matthias Urban Anne-Kathrin Dietel Andrea Csáki Wolfgang Fritzsche Cost‐Effective and Robust Multispectral Light‐Emitting Diode Device for the Readout of Plasmonic Microarray Sensors Advanced Photonics Research localized surface plasmon resonance sensors microfluidic systems multispectral light-emitting diode readout nanoparticle spotting plasmonic microarrays |
| title | Cost‐Effective and Robust Multispectral Light‐Emitting Diode Device for the Readout of Plasmonic Microarray Sensors |
| title_full | Cost‐Effective and Robust Multispectral Light‐Emitting Diode Device for the Readout of Plasmonic Microarray Sensors |
| title_fullStr | Cost‐Effective and Robust Multispectral Light‐Emitting Diode Device for the Readout of Plasmonic Microarray Sensors |
| title_full_unstemmed | Cost‐Effective and Robust Multispectral Light‐Emitting Diode Device for the Readout of Plasmonic Microarray Sensors |
| title_short | Cost‐Effective and Robust Multispectral Light‐Emitting Diode Device for the Readout of Plasmonic Microarray Sensors |
| title_sort | cost effective and robust multispectral light emitting diode device for the readout of plasmonic microarray sensors |
| topic | localized surface plasmon resonance sensors microfluidic systems multispectral light-emitting diode readout nanoparticle spotting plasmonic microarrays |
| url | https://doi.org/10.1002/adpr.202200252 |
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