Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function
Abstract Background Electroacupuncture is well known for its advantageous neuroanalgesic and therapeutic effects on myocardial ischemia–reperfusion injury. The purpose of the present research was to verify whether electroacupuncture can alleviate bupivacaine-induced myocardial injury. Methods Specif...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-08-01
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| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40001-020-00431-4 |
| _version_ | 1818172674376990720 |
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| author | Chunai Wang Xi Liang Yan Yu Yulan Li Xiaohui Wen Min Liu |
| author_facet | Chunai Wang Xi Liang Yan Yu Yulan Li Xiaohui Wen Min Liu |
| author_sort | Chunai Wang |
| collection | DOAJ |
| description | Abstract Background Electroacupuncture is well known for its advantageous neuroanalgesic and therapeutic effects on myocardial ischemia–reperfusion injury. The purpose of the present research was to verify whether electroacupuncture can alleviate bupivacaine-induced myocardial injury. Methods Specific pathogen-free Wistar rats were used to establish the bupivacaine-induced myocardial injury model. Western blot, PCR, transmission electron microscope and enzyme-linked immunosorbent (ELISA) methods were used to evaluate bupivacaine-induced structure injury and dysfunction of the mitochondria as well as the alleviating effects of lipid emulsion, acupoint injection, and electroacupuncture pre-treatment of the oxidase stress response. Results Bupivacaine caused structural damage, degradation, and swelling of mitochondria. Furthermore, it reduced adenosine triphosphate (ATP) synthesis and impaired energy metabolism in the mitochondria. Structural and functional impairment of the mitochondria was alleviated via lipid emulsion injection, acupoint injection, and electroacupuncture pre-treatment. Electroacupuncture pre-treatment of PC6 yielded a greater alleviating effect than others approaches. Following electroacupuncture pre-treatment of PC6 point, the number of mitochondria increased; apoptosis was reduced, enzymatic activity of cytochrome C oxidase (COX) and superoxide dismutase and expression of uncoupling protein 2, voltage-dependent anion channel 1, and Bcl 2 were upregulated and SLC25A6, MDA levels were downregulated. Additionally, our findings indicated that electroacupuncture pre-treatment of PC6 point exerted an effect on the mitochondria via the mitochondrial-transcription-factor-A/nuclear-respiratory-factor-1/proliferator-activated-receptor-gamma-coactivator-1 pathway. Conclusion The present study revealed that electroacupuncture pre-treatment of PC6 could effectively alleviate bupivacaine-induced myocardial mitochondrial damage, thereby providing a theoretical basis for clinical studies and applications of this treatment method. |
| first_indexed | 2024-12-11T19:16:22Z |
| format | Article |
| id | doaj.art-29e706b28ab04e1b80ad880bd05fd8be |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-12-11T19:16:22Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-29e706b28ab04e1b80ad880bd05fd8be2022-12-22T00:53:38ZengBMCEuropean Journal of Medical Research2047-783X2020-08-0125111010.1186/s40001-020-00431-4Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial functionChunai Wang0Xi Liang1Yan Yu2Yulan Li3Xiaohui Wen4Min Liu5Gansu Provincial Hospital of Traditional Chinese MedicineGansu Provincial Hospital of Traditional Chinese MedicineGansu Provincial Hospital of Traditional Chinese MedicineThe First Hospital of Lanzhou UniversityGansu University of Chinese MedicineAffiliated Hospital of Gansu University of Chinese MedicineAbstract Background Electroacupuncture is well known for its advantageous neuroanalgesic and therapeutic effects on myocardial ischemia–reperfusion injury. The purpose of the present research was to verify whether electroacupuncture can alleviate bupivacaine-induced myocardial injury. Methods Specific pathogen-free Wistar rats were used to establish the bupivacaine-induced myocardial injury model. Western blot, PCR, transmission electron microscope and enzyme-linked immunosorbent (ELISA) methods were used to evaluate bupivacaine-induced structure injury and dysfunction of the mitochondria as well as the alleviating effects of lipid emulsion, acupoint injection, and electroacupuncture pre-treatment of the oxidase stress response. Results Bupivacaine caused structural damage, degradation, and swelling of mitochondria. Furthermore, it reduced adenosine triphosphate (ATP) synthesis and impaired energy metabolism in the mitochondria. Structural and functional impairment of the mitochondria was alleviated via lipid emulsion injection, acupoint injection, and electroacupuncture pre-treatment. Electroacupuncture pre-treatment of PC6 yielded a greater alleviating effect than others approaches. Following electroacupuncture pre-treatment of PC6 point, the number of mitochondria increased; apoptosis was reduced, enzymatic activity of cytochrome C oxidase (COX) and superoxide dismutase and expression of uncoupling protein 2, voltage-dependent anion channel 1, and Bcl 2 were upregulated and SLC25A6, MDA levels were downregulated. Additionally, our findings indicated that electroacupuncture pre-treatment of PC6 point exerted an effect on the mitochondria via the mitochondrial-transcription-factor-A/nuclear-respiratory-factor-1/proliferator-activated-receptor-gamma-coactivator-1 pathway. Conclusion The present study revealed that electroacupuncture pre-treatment of PC6 could effectively alleviate bupivacaine-induced myocardial mitochondrial damage, thereby providing a theoretical basis for clinical studies and applications of this treatment method.http://link.springer.com/article/10.1186/s40001-020-00431-4BupivacaineElectroacupuncture preconditioningCardiac mitochondriaNeiguan pointLipid emulsion |
| spellingShingle | Chunai Wang Xi Liang Yan Yu Yulan Li Xiaohui Wen Min Liu Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function European Journal of Medical Research Bupivacaine Electroacupuncture preconditioning Cardiac mitochondria Neiguan point Lipid emulsion |
| title | Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function |
| title_full | Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function |
| title_fullStr | Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function |
| title_full_unstemmed | Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function |
| title_short | Electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function |
| title_sort | electroacupuncture pretreatment alleviates myocardial injury through regulating mitochondrial function |
| topic | Bupivacaine Electroacupuncture preconditioning Cardiac mitochondria Neiguan point Lipid emulsion |
| url | http://link.springer.com/article/10.1186/s40001-020-00431-4 |
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