Undefined cellulase formulations hinder scientific reproducibility
Abstract In the shadow of a burgeoning biomass-to-fuels industry, biological conversion of lignocellulose to fermentable sugars in a cost-effective manner is key to the success of second-generation and advanced biofuel production. For the effective comparison of one cellulase preparation to another,...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-11-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-017-0974-y |
| _version_ | 1818209222030000128 |
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| author | Michael E. Himmel Charles A. Abbas John O. Baker Edward A. Bayer Yannick J. Bomble Roman Brunecky Xiaowen Chen Claus Felby Tina Jeoh Rajeev Kumar Barry V. McCleary Brett I. Pletschke Melvin P. Tucker Charles E. Wyman Stephen R. Decker |
| author_facet | Michael E. Himmel Charles A. Abbas John O. Baker Edward A. Bayer Yannick J. Bomble Roman Brunecky Xiaowen Chen Claus Felby Tina Jeoh Rajeev Kumar Barry V. McCleary Brett I. Pletschke Melvin P. Tucker Charles E. Wyman Stephen R. Decker |
| author_sort | Michael E. Himmel |
| collection | DOAJ |
| description | Abstract In the shadow of a burgeoning biomass-to-fuels industry, biological conversion of lignocellulose to fermentable sugars in a cost-effective manner is key to the success of second-generation and advanced biofuel production. For the effective comparison of one cellulase preparation to another, cellulase assays are typically carried out with one or more engineered cellulase formulations or natural exoproteomes of known performance serving as positive controls. When these formulations have unknown composition, as is the case with several widely used commercial products, it becomes impossible to compare or reproduce work done today to work done in the future, where, for example, such preparations may not be available. Therefore, being a critical tenet of science publishing, experimental reproducibility is endangered by the continued use of these undisclosed products. We propose the introduction of standard procedures and materials to produce specific and reproducible cellulase formulations. These formulations are to serve as yardsticks to measure improvements and performance of new cellulase formulations. |
| first_indexed | 2024-12-12T04:57:16Z |
| format | Article |
| id | doaj.art-29ea8c0cc8d84d79aa253c7179491a05 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T04:57:16Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-29ea8c0cc8d84d79aa253c7179491a052022-12-22T00:37:18ZengBMCBiotechnology for Biofuels1754-68342017-11-011011410.1186/s13068-017-0974-yUndefined cellulase formulations hinder scientific reproducibilityMichael E. Himmel0Charles A. Abbas1John O. Baker2Edward A. Bayer3Yannick J. Bomble4Roman Brunecky5Xiaowen Chen6Claus Felby7Tina Jeoh8Rajeev Kumar9Barry V. McCleary10Brett I. Pletschke11Melvin P. Tucker12Charles E. Wyman13Stephen R. Decker14Biosciences Center, National Renewable Energy LaboratoryDepartment of Food Science and Human Nutrition, University of IllinoisBiosciences Center, National Renewable Energy LaboratoryDepartment of Biomolecular Sciences, The Weizmann Institute of ScienceBiosciences Center, National Renewable Energy LaboratoryBiosciences Center, National Renewable Energy LaboratoryNational Bioenergy Center, National Renewable Energy LaboratoryDepartment of Geosciences and Natural Resource Management, University of CopenhagenDepartment of Biological and Agricultural Engineering, University of California DavisBourns College of Engineering Center for Environmental Research and Technology, University of California, RiversideMegazymeDepartment of Biochemistry and Microbiology, Rhodes UniversityDepartment of Food Science and Human Nutrition, University of IllinoisBourns College of Engineering Center for Environmental Research and Technology, University of California, RiversideBiosciences Center, National Renewable Energy LaboratoryAbstract In the shadow of a burgeoning biomass-to-fuels industry, biological conversion of lignocellulose to fermentable sugars in a cost-effective manner is key to the success of second-generation and advanced biofuel production. For the effective comparison of one cellulase preparation to another, cellulase assays are typically carried out with one or more engineered cellulase formulations or natural exoproteomes of known performance serving as positive controls. When these formulations have unknown composition, as is the case with several widely used commercial products, it becomes impossible to compare or reproduce work done today to work done in the future, where, for example, such preparations may not be available. Therefore, being a critical tenet of science publishing, experimental reproducibility is endangered by the continued use of these undisclosed products. We propose the introduction of standard procedures and materials to produce specific and reproducible cellulase formulations. These formulations are to serve as yardsticks to measure improvements and performance of new cellulase formulations.http://link.springer.com/article/10.1186/s13068-017-0974-yCellulaseCelluloseBiofuelsAssaysCommercial cellulase formulations |
| spellingShingle | Michael E. Himmel Charles A. Abbas John O. Baker Edward A. Bayer Yannick J. Bomble Roman Brunecky Xiaowen Chen Claus Felby Tina Jeoh Rajeev Kumar Barry V. McCleary Brett I. Pletschke Melvin P. Tucker Charles E. Wyman Stephen R. Decker Undefined cellulase formulations hinder scientific reproducibility Biotechnology for Biofuels Cellulase Cellulose Biofuels Assays Commercial cellulase formulations |
| title | Undefined cellulase formulations hinder scientific reproducibility |
| title_full | Undefined cellulase formulations hinder scientific reproducibility |
| title_fullStr | Undefined cellulase formulations hinder scientific reproducibility |
| title_full_unstemmed | Undefined cellulase formulations hinder scientific reproducibility |
| title_short | Undefined cellulase formulations hinder scientific reproducibility |
| title_sort | undefined cellulase formulations hinder scientific reproducibility |
| topic | Cellulase Cellulose Biofuels Assays Commercial cellulase formulations |
| url | http://link.springer.com/article/10.1186/s13068-017-0974-y |
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