Microbial Ecotoxicity of Biochars in Agricultural Soil and Interactions with Linear Alkylbenzene Sulfonates
Large-scale application of biochar on agricultural land offers the prospect of soil improvement and carbon sequestration for climate-change mitigation. However, negative side-effects on the soil microbial ecosystem are poorly understood, notably in relation to the functions of native microbiomes und...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/5/828 |
| _version_ | 1797536634048086016 |
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| author | Rasmus Nissen Gunadhish Khanal Lars Elsgaard |
| author_facet | Rasmus Nissen Gunadhish Khanal Lars Elsgaard |
| author_sort | Rasmus Nissen |
| collection | DOAJ |
| description | Large-scale application of biochar on agricultural land offers the prospect of soil improvement and carbon sequestration for climate-change mitigation. However, negative side-effects on the soil microbial ecosystem are poorly understood, notably in relation to the functions of native microbiomes under realistic routes of biochar exposure. Due to divergent properties, different biochars might interact with soil in complex ways. This might result in decreased or increased ecotoxicity from biochar contaminants, such as heavy metals and polycyclic aromatic hydrocarbons (PAHs). Using five biochars produced from straw and wood under contrasting pyrolysis conditions, we traced their ecotoxicological dose-effect using a bioassay for potential ammonia oxidation (PAO), through microorganisms that are sensitive stress indicators. Assays were made after soil/biochar interaction for up to 3 weeks, where straw biochar with the lowest PAH content (<0.5 mg kg<sup>−1</sup>) showed the most pronounced dose-effects to PAO, corresponding to a 10% effect concentration (EC<sub>10</sub>) of 4.6% (dry weight biochar/dry weight soil). In comparison, straw biochar with the highest PAH content was least ecotoxic (EC<sub>10</sub>, 15.2% after 3 weeks) and wood biochars pyrolysed at high temperature (700–725 °C) showed no ecotoxicity to PAO. Interactions between biochars and anionic surfactants, i.e., linear alkylbenzene sulfonates, which are common soil pollutants, resulted in varying effects on PAO, but the effects were small and of limited ecological importance for soil-amended biochars. In conclusion, the results showed that short-term microbial side-effects of biochar in the soil ecosystem were minor at relevant field application rates (such as <30 Mg ha<sup>−1</sup> mixed into a plough layer of 20 cm), and that inherent PAHs in biochar were not a likely source of short-term ecotoxicity. However, there were notable differences in the effects eventually observed at very high biochar rates, stressing that individual biochars need specific ecotoxicological assessment before their safe application at large scale in agricultural soils. |
| first_indexed | 2024-03-10T12:02:34Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T12:02:34Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-29eabb355156498aa9a1ed67a78995ee2023-11-21T16:47:14ZengMDPI AGAgronomy2073-43952021-04-0111582810.3390/agronomy11050828Microbial Ecotoxicity of Biochars in Agricultural Soil and Interactions with Linear Alkylbenzene SulfonatesRasmus Nissen0Gunadhish Khanal1Lars Elsgaard2Department of Agroecology, Aarhus University, Blichers Allé 20, DK-8830 Tjele, DenmarkDepartment of Agroecology, Aarhus University, Blichers Allé 20, DK-8830 Tjele, DenmarkDepartment of Agroecology, Aarhus University, Blichers Allé 20, DK-8830 Tjele, DenmarkLarge-scale application of biochar on agricultural land offers the prospect of soil improvement and carbon sequestration for climate-change mitigation. However, negative side-effects on the soil microbial ecosystem are poorly understood, notably in relation to the functions of native microbiomes under realistic routes of biochar exposure. Due to divergent properties, different biochars might interact with soil in complex ways. This might result in decreased or increased ecotoxicity from biochar contaminants, such as heavy metals and polycyclic aromatic hydrocarbons (PAHs). Using five biochars produced from straw and wood under contrasting pyrolysis conditions, we traced their ecotoxicological dose-effect using a bioassay for potential ammonia oxidation (PAO), through microorganisms that are sensitive stress indicators. Assays were made after soil/biochar interaction for up to 3 weeks, where straw biochar with the lowest PAH content (<0.5 mg kg<sup>−1</sup>) showed the most pronounced dose-effects to PAO, corresponding to a 10% effect concentration (EC<sub>10</sub>) of 4.6% (dry weight biochar/dry weight soil). In comparison, straw biochar with the highest PAH content was least ecotoxic (EC<sub>10</sub>, 15.2% after 3 weeks) and wood biochars pyrolysed at high temperature (700–725 °C) showed no ecotoxicity to PAO. Interactions between biochars and anionic surfactants, i.e., linear alkylbenzene sulfonates, which are common soil pollutants, resulted in varying effects on PAO, but the effects were small and of limited ecological importance for soil-amended biochars. In conclusion, the results showed that short-term microbial side-effects of biochar in the soil ecosystem were minor at relevant field application rates (such as <30 Mg ha<sup>−1</sup> mixed into a plough layer of 20 cm), and that inherent PAHs in biochar were not a likely source of short-term ecotoxicity. However, there were notable differences in the effects eventually observed at very high biochar rates, stressing that individual biochars need specific ecotoxicological assessment before their safe application at large scale in agricultural soils.https://www.mdpi.com/2073-4395/11/5/828biocharsoilecotoxicityLASammonia oxidationmicroorganisms |
| spellingShingle | Rasmus Nissen Gunadhish Khanal Lars Elsgaard Microbial Ecotoxicity of Biochars in Agricultural Soil and Interactions with Linear Alkylbenzene Sulfonates Agronomy biochar soil ecotoxicity LAS ammonia oxidation microorganisms |
| title | Microbial Ecotoxicity of Biochars in Agricultural Soil and Interactions with Linear Alkylbenzene Sulfonates |
| title_full | Microbial Ecotoxicity of Biochars in Agricultural Soil and Interactions with Linear Alkylbenzene Sulfonates |
| title_fullStr | Microbial Ecotoxicity of Biochars in Agricultural Soil and Interactions with Linear Alkylbenzene Sulfonates |
| title_full_unstemmed | Microbial Ecotoxicity of Biochars in Agricultural Soil and Interactions with Linear Alkylbenzene Sulfonates |
| title_short | Microbial Ecotoxicity of Biochars in Agricultural Soil and Interactions with Linear Alkylbenzene Sulfonates |
| title_sort | microbial ecotoxicity of biochars in agricultural soil and interactions with linear alkylbenzene sulfonates |
| topic | biochar soil ecotoxicity LAS ammonia oxidation microorganisms |
| url | https://www.mdpi.com/2073-4395/11/5/828 |
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