Model Construction and Analysis of Respiration in Halobacterium salinarum.
The archaeon Halobacterium salinarum can produce energy using three different processes, namely photosynthesis, oxidative phosphorylation and fermentation of arginine, and is thus a model organism in bioenergetics. Compared to its bacteriorhodopsin-driven photosynthesis, less attention has been devo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4806987?pdf=render |
| _version_ | 1811207028929986560 |
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| author | Cherryl O Talaue Ricardo C H del Rosario Friedhelm Pfeiffer Eduardo R Mendoza Dieter Oesterhelt |
| author_facet | Cherryl O Talaue Ricardo C H del Rosario Friedhelm Pfeiffer Eduardo R Mendoza Dieter Oesterhelt |
| author_sort | Cherryl O Talaue |
| collection | DOAJ |
| description | The archaeon Halobacterium salinarum can produce energy using three different processes, namely photosynthesis, oxidative phosphorylation and fermentation of arginine, and is thus a model organism in bioenergetics. Compared to its bacteriorhodopsin-driven photosynthesis, less attention has been devoted to modeling its respiratory pathway. We created a system of ordinary differential equations that models its oxidative phosphorylation. The model consists of the electron transport chain, the ATP synthase, the potassium uniport and the sodium-proton antiport. By fitting the model parameters to experimental data, we show that the model can explain data on proton motive force generation, ATP production, and the charge balancing of ions between the sodium-proton antiporter and the potassium uniport. We performed sensitivity analysis of the model parameters to determine how the model will respond to perturbations in parameter values. The model and the parameters we derived provide a resource that can be used for analytical studies of the bioenergetics of H. salinarum. |
| first_indexed | 2024-04-12T03:56:55Z |
| format | Article |
| id | doaj.art-29ef284ff1574015a45da033e61151b6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T03:56:55Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-29ef284ff1574015a45da033e61151b62022-12-22T03:48:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015183910.1371/journal.pone.0151839Model Construction and Analysis of Respiration in Halobacterium salinarum.Cherryl O TalaueRicardo C H del RosarioFriedhelm PfeifferEduardo R MendozaDieter OesterheltThe archaeon Halobacterium salinarum can produce energy using three different processes, namely photosynthesis, oxidative phosphorylation and fermentation of arginine, and is thus a model organism in bioenergetics. Compared to its bacteriorhodopsin-driven photosynthesis, less attention has been devoted to modeling its respiratory pathway. We created a system of ordinary differential equations that models its oxidative phosphorylation. The model consists of the electron transport chain, the ATP synthase, the potassium uniport and the sodium-proton antiport. By fitting the model parameters to experimental data, we show that the model can explain data on proton motive force generation, ATP production, and the charge balancing of ions between the sodium-proton antiporter and the potassium uniport. We performed sensitivity analysis of the model parameters to determine how the model will respond to perturbations in parameter values. The model and the parameters we derived provide a resource that can be used for analytical studies of the bioenergetics of H. salinarum.http://europepmc.org/articles/PMC4806987?pdf=render |
| spellingShingle | Cherryl O Talaue Ricardo C H del Rosario Friedhelm Pfeiffer Eduardo R Mendoza Dieter Oesterhelt Model Construction and Analysis of Respiration in Halobacterium salinarum. PLoS ONE |
| title | Model Construction and Analysis of Respiration in Halobacterium salinarum. |
| title_full | Model Construction and Analysis of Respiration in Halobacterium salinarum. |
| title_fullStr | Model Construction and Analysis of Respiration in Halobacterium salinarum. |
| title_full_unstemmed | Model Construction and Analysis of Respiration in Halobacterium salinarum. |
| title_short | Model Construction and Analysis of Respiration in Halobacterium salinarum. |
| title_sort | model construction and analysis of respiration in halobacterium salinarum |
| url | http://europepmc.org/articles/PMC4806987?pdf=render |
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