The Effect of Melon Seed Extract on the Transcription of NF-κB and Myeloperoxidase in the Liver Tissue of Mice Poisoned with Ethylene Glycol
Background and Objectives: The liver is exposed to poisoning caused by various chemical compounds. Considering the antioxidant effects found in melon seeds and the role of nuclear factor kappa B (NF-κB) and Myeloperoxidase (MPO) in inflammation caused by oxidative stress, the present study aims to a...
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| Format: | Article |
| Language: | fas |
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Qom University of Medical Sciences
2022-11-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| Subjects: | |
| Online Access: | http://journal.muq.ac.ir/article-1-3517-en.html |
| _version_ | 1797845456043114496 |
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| author | Mohamad-Reza Forouharmanesh Mehdi Ebrahimi Maryam Eidi |
| author_facet | Mohamad-Reza Forouharmanesh Mehdi Ebrahimi Maryam Eidi |
| author_sort | Mohamad-Reza Forouharmanesh |
| collection | DOAJ |
| description | Background and Objectives: The liver is exposed to poisoning caused by various chemical compounds. Considering the antioxidant effects found in melon seeds and the role of nuclear factor kappa B (NF-κB) and Myeloperoxidase (MPO) in inflammation caused by oxidative stress, the present study aims to assess the effect of hydroethanolic extract of melon seeds on the transcriptional of NF-κB encoding genes and the MPO gene expression in rats with ethylene glycol-induced toxicity.
Methods: Thirty male rats were randomly divided into five groups of healthy control, poisoned control (0.75% ethylene glycol), and three melon extract-treated poisoned groups (0.75% ethylene glycol and 150, 300, or 600 mg/kg body weight of melon extract) and were treated for 38 days. Then, the liver tissue of the rats was removed to evaluate the transcription of NF-κB encoding genes and the MPO level by real-time PCR method.
Results: The transcription level of NF-κB encoding genes and the MPO level in the poisoned control group rats significantly increased compared to the healthy group. Treatment with hydroethanolic extract of melon seeds at concentrations of 300 and 600 mg/kg body weight caused a significant decrease in the transcription of NF-κB encoding genes. Treatment with hydroethanolic extracts at concentrations of 150, 300, and 600 mg/kg body weight caused a significant decrease in the MPO level compared to the poisoned control group.
Conclusion: The decrease in MPO and NF-κB encoding genes after administration of hydroethanolic extract of melon seeds indicates the therapeutic effect of this extract on liver damage. |
| first_indexed | 2024-04-09T17:39:20Z |
| format | Article |
| id | doaj.art-29f2dd67abcc4cf5aa1a2d490a5b0786 |
| institution | Directory Open Access Journal |
| issn | 1735-7799 2008-1375 |
| language | fas |
| last_indexed | 2024-04-09T17:39:20Z |
| publishDate | 2022-11-01 |
| publisher | Qom University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| spelling | doaj.art-29f2dd67abcc4cf5aa1a2d490a5b07862023-04-17T06:30:11ZfasQom University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum1735-77992008-13752022-11-01168664675The Effect of Melon Seed Extract on the Transcription of NF-κB and Myeloperoxidase in the Liver Tissue of Mice Poisoned with Ethylene GlycolMohamad-Reza Forouharmanesh0Mehdi Ebrahimi1Maryam Eidi2 Department of Genetics, College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Pishva, Iran. Department of Biochemistry and Biophysics, College of Biological Sciences, Varamin-Pishva branch, Islamic Azad University, Pishva, Iran. Department of Biology, College of Biological Sciences, Varamin-Pishva Branch, Islamic Azad University, Pishva, Iran. Background and Objectives: The liver is exposed to poisoning caused by various chemical compounds. Considering the antioxidant effects found in melon seeds and the role of nuclear factor kappa B (NF-κB) and Myeloperoxidase (MPO) in inflammation caused by oxidative stress, the present study aims to assess the effect of hydroethanolic extract of melon seeds on the transcriptional of NF-κB encoding genes and the MPO gene expression in rats with ethylene glycol-induced toxicity. Methods: Thirty male rats were randomly divided into five groups of healthy control, poisoned control (0.75% ethylene glycol), and three melon extract-treated poisoned groups (0.75% ethylene glycol and 150, 300, or 600 mg/kg body weight of melon extract) and were treated for 38 days. Then, the liver tissue of the rats was removed to evaluate the transcription of NF-κB encoding genes and the MPO level by real-time PCR method. Results: The transcription level of NF-κB encoding genes and the MPO level in the poisoned control group rats significantly increased compared to the healthy group. Treatment with hydroethanolic extract of melon seeds at concentrations of 300 and 600 mg/kg body weight caused a significant decrease in the transcription of NF-κB encoding genes. Treatment with hydroethanolic extracts at concentrations of 150, 300, and 600 mg/kg body weight caused a significant decrease in the MPO level compared to the poisoned control group. Conclusion: The decrease in MPO and NF-κB encoding genes after administration of hydroethanolic extract of melon seeds indicates the therapeutic effect of this extract on liver damage.http://journal.muq.ac.ir/article-1-3517-en.htmlmelonethylene glycolmyeloperoxidasenf-kb |
| spellingShingle | Mohamad-Reza Forouharmanesh Mehdi Ebrahimi Maryam Eidi The Effect of Melon Seed Extract on the Transcription of NF-κB and Myeloperoxidase in the Liver Tissue of Mice Poisoned with Ethylene Glycol Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum melon ethylene glycol myeloperoxidase nf-kb |
| title | The Effect of Melon Seed Extract on the Transcription of NF-κB and Myeloperoxidase in the Liver Tissue of Mice Poisoned with Ethylene Glycol |
| title_full | The Effect of Melon Seed Extract on the Transcription of NF-κB and Myeloperoxidase in the Liver Tissue of Mice Poisoned with Ethylene Glycol |
| title_fullStr | The Effect of Melon Seed Extract on the Transcription of NF-κB and Myeloperoxidase in the Liver Tissue of Mice Poisoned with Ethylene Glycol |
| title_full_unstemmed | The Effect of Melon Seed Extract on the Transcription of NF-κB and Myeloperoxidase in the Liver Tissue of Mice Poisoned with Ethylene Glycol |
| title_short | The Effect of Melon Seed Extract on the Transcription of NF-κB and Myeloperoxidase in the Liver Tissue of Mice Poisoned with Ethylene Glycol |
| title_sort | effect of melon seed extract on the transcription of nf κb and myeloperoxidase in the liver tissue of mice poisoned with ethylene glycol |
| topic | melon ethylene glycol myeloperoxidase nf-kb |
| url | http://journal.muq.ac.ir/article-1-3517-en.html |
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