Insecticide induction of O-demethylase activity and expression of cytochrome P450 genes in the red imported fire ant (Solenopsis invicta Buren)
The red imported fire ant (Solenopsis invicta) is a global major invasive pest, and has caused significant economic, social and environmental impacts since its invasion to mainland of China in 2004. To date, chemical control has been the most effective measure. However, the long-term use of chemical...
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| Language: | English |
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Elsevier
2016-01-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915610723 |
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| author | Bai-zhong ZHANG Fan-chao KONG Hua-tang WANG Xi-wu GAO Xin-nian ZENG Xue-yan SHI |
| author_facet | Bai-zhong ZHANG Fan-chao KONG Hua-tang WANG Xi-wu GAO Xin-nian ZENG Xue-yan SHI |
| author_sort | Bai-zhong ZHANG |
| collection | DOAJ |
| description | The red imported fire ant (Solenopsis invicta) is a global major invasive pest, and has caused significant economic, social and environmental impacts since its invasion to mainland of China in 2004. To date, chemical control has been the most effective measure. However, the long-term use of chemicals would lead to an unexpected rebound. To understand the risks and explore the mechanisms of detoxification or induction to insecticides in S. invicta, the O-demethylase activity and expression of cytochrome P450 genes of workers and queens, and the effects of chlorpyrifos and fipronil exposure in workers were investigated. Biochemical assays showed the O-demethylase activity of cytochrome P450 was significantly higher in workers than in queens (1.66-fold), and was significantly induced in workers exposed to chlorpyrifos and fipronil, reaching a maximum (3.00- and 1.95-fold) at 48 h and then decreasing dramatically compared to controls (exposed to acetone counter-part). The relative expression levels of 12 cytochrome P450 expressed sequence tags (ESTs) in workers were significantly higher than in queens (from 2.3- to 36.4-fold). Multiple cytochrome P450 genes (except 9E4) were co-up-regulated (from 1.5- to 2.86-fold) in workers exposed to fipronil. These results indicated that the increased O-demethylase activity may result from the increased transcription levels of cytochrome P450 related to detoxification of insecticides in S. invicta. It appears that cytochrome P450 plays an important role in enhanced metabolic detoxification of insecticides. At the same time, it also provides the theoretical basis for resistance management and rational usage of insecticides to control S. invicta. |
| first_indexed | 2024-12-18T23:24:39Z |
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| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-18T23:24:39Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-29f4f4f740ec47ce903e1c92dcc9d41f2022-12-21T20:47:51ZengElsevierJournal of Integrative Agriculture2095-31192016-01-01151135144Insecticide induction of O-demethylase activity and expression of cytochrome P450 genes in the red imported fire ant (Solenopsis invicta Buren)Bai-zhong ZHANG0Fan-chao KONG1Hua-tang WANG2Xi-wu GAO3Xin-nian ZENG4Xue-yan SHI5College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R.China; Correspondence GAO Xi-wu, Tel: +86-10-62732974College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R.ChinaCollege of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R.ChinaDepartment of Entomology, China Agricultural University, Beijing 100193, P.R.China; Correspondence GAO Xi-wu, Tel: +86-10-62732974College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R.China; ZENG Xin-nian, Tel: +86-20-85284925Department of Entomology, China Agricultural University, Beijing 100193, P.R.ChinaThe red imported fire ant (Solenopsis invicta) is a global major invasive pest, and has caused significant economic, social and environmental impacts since its invasion to mainland of China in 2004. To date, chemical control has been the most effective measure. However, the long-term use of chemicals would lead to an unexpected rebound. To understand the risks and explore the mechanisms of detoxification or induction to insecticides in S. invicta, the O-demethylase activity and expression of cytochrome P450 genes of workers and queens, and the effects of chlorpyrifos and fipronil exposure in workers were investigated. Biochemical assays showed the O-demethylase activity of cytochrome P450 was significantly higher in workers than in queens (1.66-fold), and was significantly induced in workers exposed to chlorpyrifos and fipronil, reaching a maximum (3.00- and 1.95-fold) at 48 h and then decreasing dramatically compared to controls (exposed to acetone counter-part). The relative expression levels of 12 cytochrome P450 expressed sequence tags (ESTs) in workers were significantly higher than in queens (from 2.3- to 36.4-fold). Multiple cytochrome P450 genes (except 9E4) were co-up-regulated (from 1.5- to 2.86-fold) in workers exposed to fipronil. These results indicated that the increased O-demethylase activity may result from the increased transcription levels of cytochrome P450 related to detoxification of insecticides in S. invicta. It appears that cytochrome P450 plays an important role in enhanced metabolic detoxification of insecticides. At the same time, it also provides the theoretical basis for resistance management and rational usage of insecticides to control S. invicta.http://www.sciencedirect.com/science/article/pii/S2095311915610723Solenopsis invictaP450 activitygene expressionchlorpyrifosfipronilinduction |
| spellingShingle | Bai-zhong ZHANG Fan-chao KONG Hua-tang WANG Xi-wu GAO Xin-nian ZENG Xue-yan SHI Insecticide induction of O-demethylase activity and expression of cytochrome P450 genes in the red imported fire ant (Solenopsis invicta Buren) Journal of Integrative Agriculture Solenopsis invicta P450 activity gene expression chlorpyrifos fipronil induction |
| title | Insecticide induction of O-demethylase activity and expression of cytochrome P450 genes in the red imported fire ant (Solenopsis invicta Buren) |
| title_full | Insecticide induction of O-demethylase activity and expression of cytochrome P450 genes in the red imported fire ant (Solenopsis invicta Buren) |
| title_fullStr | Insecticide induction of O-demethylase activity and expression of cytochrome P450 genes in the red imported fire ant (Solenopsis invicta Buren) |
| title_full_unstemmed | Insecticide induction of O-demethylase activity and expression of cytochrome P450 genes in the red imported fire ant (Solenopsis invicta Buren) |
| title_short | Insecticide induction of O-demethylase activity and expression of cytochrome P450 genes in the red imported fire ant (Solenopsis invicta Buren) |
| title_sort | insecticide induction of o demethylase activity and expression of cytochrome p450 genes in the red imported fire ant solenopsis invicta buren |
| topic | Solenopsis invicta P450 activity gene expression chlorpyrifos fipronil induction |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915610723 |
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