Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy
Twenty years after its development, antibody phage display using filamentous bacteriophage represents the most successful in vitro antibody selection technology. Initially, its development was encouraged by the unique possibility of directly generating recombinant human antibodies for therapy. Today...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2011-01-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/16/1/412/ |
| _version_ | 1811312687367323648 |
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| author | Michael Hust André Frenzel Mark Schütte Torsten Meyer Thomas Schirrmann |
| author_facet | Michael Hust André Frenzel Mark Schütte Torsten Meyer Thomas Schirrmann |
| author_sort | Michael Hust |
| collection | DOAJ |
| description | Twenty years after its development, antibody phage display using filamentous bacteriophage represents the most successful in vitro antibody selection technology. Initially, its development was encouraged by the unique possibility of directly generating recombinant human antibodies for therapy. Today, antibody phage display has been developed as a robust technology offering great potential for automation. Generation of monospecific binders provides a valuable tool for proteome research, leading to highly enhanced throughput and reduced costs. This review presents the phage display technology, application areas of antibodies in research, diagnostics and therapy and the use of antibody phage display for these applications. |
| first_indexed | 2024-04-13T10:41:14Z |
| format | Article |
| id | doaj.art-29fad46292a8466eafd85b2381ff5c82 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T10:41:14Z |
| publishDate | 2011-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-29fad46292a8466eafd85b2381ff5c822022-12-22T02:49:55ZengMDPI AGMolecules1420-30492011-01-0116141242610.3390/molecules16010412Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and TherapyMichael HustAndré FrenzelMark SchütteTorsten MeyerThomas SchirrmannTwenty years after its development, antibody phage display using filamentous bacteriophage represents the most successful in vitro antibody selection technology. Initially, its development was encouraged by the unique possibility of directly generating recombinant human antibodies for therapy. Today, antibody phage display has been developed as a robust technology offering great potential for automation. Generation of monospecific binders provides a valuable tool for proteome research, leading to highly enhanced throughput and reduced costs. This review presents the phage display technology, application areas of antibodies in research, diagnostics and therapy and the use of antibody phage display for these applications.http://www.mdpi.com/1420-3049/16/1/412/antibodyscFvphage displaycancerdiagnosticsproteome researchpanning |
| spellingShingle | Michael Hust André Frenzel Mark Schütte Torsten Meyer Thomas Schirrmann Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy Molecules antibody scFv phage display cancer diagnostics proteome research panning |
| title | Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy |
| title_full | Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy |
| title_fullStr | Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy |
| title_full_unstemmed | Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy |
| title_short | Phage Display for the Generation of Antibodies for Proteome Research, Diagnostics and Therapy |
| title_sort | phage display for the generation of antibodies for proteome research diagnostics and therapy |
| topic | antibody scFv phage display cancer diagnostics proteome research panning |
| url | http://www.mdpi.com/1420-3049/16/1/412/ |
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