Effect of water content and temperature on Carica papaya lipase catalyzed esterification and transesterification reactions

Temperature and water activity (a w) of the reaction medium are two factors that govern enzyme reactions. We studied the influence of these two parameters on the esterification and transesterification activity of Carica papaya lipase in water and solvent free reactions. It was found that over t...

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Bibliographic Details
Main Authors: Turon Fabrice, Caro Yanis, Villeneuve Pierre, Pina Michel, Graille Jean
Format: Article
Language:English
Published: EDP Sciences 2003-09-01
Series:Oléagineux, Corps gras, Lipides
Subjects:
Online Access:http://dx.doi.org/10.1051/ocl.2003.0400
Description
Summary:Temperature and water activity (a w) of the reaction medium are two factors that govern enzyme reactions. We studied the influence of these two parameters on the esterification and transesterification activity of Carica papaya lipase in water and solvent free reactions. It was found that over the course of reaction the catalytic activity of C. papaya lipase was dependent on these factors. The best lipase activity for both reactions was at a temperature of 55°C and water activity of 0.22, which corresponds to 2 g of water per 100 g of C. papaya latex.
ISSN:1258-8210
1950-697X