DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic Operations
Multiplex miRNA analysis is a fundamental issue for exploring a complex biological system and early diagnosis of miRNA-related diseases. Herein, we have developed a series of novel logic gates for miRNA analysis coupling DNA nanostructures and chameleon silver nanoclusters (AgNCs). DNA dumbbell stru...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2020-01-01
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| Series: | Research |
| Online Access: | http://dx.doi.org/10.34133/2020/1091605 |
| _version_ | 1797282543415853056 |
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| author | Yiting Jiang Peng Miao |
| author_facet | Yiting Jiang Peng Miao |
| author_sort | Yiting Jiang |
| collection | DOAJ |
| description | Multiplex miRNA analysis is a fundamental issue for exploring a complex biological system and early diagnosis of miRNA-related diseases. Herein, we have developed a series of novel logic gates for miRNA analysis coupling DNA nanostructures and chameleon silver nanoclusters (AgNCs). DNA dumbbell structures are firstly designed with two independent nucleation sequences for AgNCs at the 5′ and 3′ ends, respectively. By introducing different miRNA inputs, separations of two AgNCs are controlled and the fluorescence property of AgNCs changes. By studying the ratiometric fluorescence responses, sensitive and selective analysis of multiple miRNAs can be achieved. The present work provides powerful tools for miRNA diagnostics and may also guide future DNA nanostructure-based logic gates. |
| first_indexed | 2024-03-07T17:14:36Z |
| format | Article |
| id | doaj.art-29ffd140c96241bd93e3537cac7f315e |
| institution | Directory Open Access Journal |
| issn | 2639-5274 |
| language | English |
| last_indexed | 2024-03-07T17:14:36Z |
| publishDate | 2020-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Research |
| spelling | doaj.art-29ffd140c96241bd93e3537cac7f315e2024-03-02T23:14:49ZengAmerican Association for the Advancement of Science (AAAS)Research2639-52742020-01-01202010.34133/2020/1091605DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic OperationsYiting Jiang0Peng Miao1Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China; University of Science and Technology of China, Hefei 230026, ChinaSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China; University of Science and Technology of China, Hefei 230026, ChinaMultiplex miRNA analysis is a fundamental issue for exploring a complex biological system and early diagnosis of miRNA-related diseases. Herein, we have developed a series of novel logic gates for miRNA analysis coupling DNA nanostructures and chameleon silver nanoclusters (AgNCs). DNA dumbbell structures are firstly designed with two independent nucleation sequences for AgNCs at the 5′ and 3′ ends, respectively. By introducing different miRNA inputs, separations of two AgNCs are controlled and the fluorescence property of AgNCs changes. By studying the ratiometric fluorescence responses, sensitive and selective analysis of multiple miRNAs can be achieved. The present work provides powerful tools for miRNA diagnostics and may also guide future DNA nanostructure-based logic gates.http://dx.doi.org/10.34133/2020/1091605 |
| spellingShingle | Yiting Jiang Peng Miao DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic Operations Research |
| title | DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic Operations |
| title_full | DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic Operations |
| title_fullStr | DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic Operations |
| title_full_unstemmed | DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic Operations |
| title_short | DNA Dumbbell and Chameleon Silver Nanoclusters for miRNA Logic Operations |
| title_sort | dna dumbbell and chameleon silver nanoclusters for mirna logic operations |
| url | http://dx.doi.org/10.34133/2020/1091605 |
| work_keys_str_mv | AT yitingjiang dnadumbbellandchameleonsilvernanoclustersformirnalogicoperations AT pengmiao dnadumbbellandchameleonsilvernanoclustersformirnalogicoperations |