Gender-Related Differences in Trimethylamine and Oxidative Blood Biomarkers in Cardiovascular Disease Patients
Gender differences in the burden of cardiovascular disease (CVD) have been observed worldwide. In this study, plasmatic levels of trimethylamine (TMA) and blood oxidative biomarkers have been evaluated in 358 men (89 controls and 269 CVD patients) and 189 women (64 control and 125 CVD patients). The...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/8/238 |
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| author | Laura Bordoni Donatella Fedeli Marco Piangerelli Iwona Pelikant-Malecka Adrianna Radulska Joanna J. Samulak Angelika K. Sawicka Lukasz Lewicki Leszek Kalinowski Robert A. Olek Rosita Gabbianelli |
| author_facet | Laura Bordoni Donatella Fedeli Marco Piangerelli Iwona Pelikant-Malecka Adrianna Radulska Joanna J. Samulak Angelika K. Sawicka Lukasz Lewicki Leszek Kalinowski Robert A. Olek Rosita Gabbianelli |
| author_sort | Laura Bordoni |
| collection | DOAJ |
| description | Gender differences in the burden of cardiovascular disease (CVD) have been observed worldwide. In this study, plasmatic levels of trimethylamine (TMA) and blood oxidative biomarkers have been evaluated in 358 men (89 controls and 269 CVD patients) and 189 women (64 control and 125 CVD patients). The fluorescence technique was applied to determine erythrocyte membrane fluidity using 1,6-diphenyl-1,3,5-hexatriene (DPH) and Laurdan, while lipid hydroperoxides were assessed by diphenyl−1-pyrenylphosphine (DPPP). Results show that levels of plasmatic TMA were higher in healthy men with respect to healthy women (<i>p</i> = 0.0001). Significantly lower TMA was observed in male CVD patients (0.609 ± 0.104 μM) compared to healthy male controls (0.680 ± 0.118 μM) (<i>p</i> < 0.001), while higher levels of TMA were measured in female CVD patients (0.595 ± 0.115 μM) with respect to female controls (0.529 ± 0.073 μM) (<i>p</i> < 0.001). DPPP was significantly higher in healthy control men than in women (<i>p</i> < 0.001). Male CVD patients displayed a lower value of DPPP (2777 ± 1924) compared to healthy controls (5528 ± 2222) (<i>p</i> < 0.001), while no significant changes were measured in females with or without CVD (<i>p</i> > 0.05). Membrane fluidity was significantly higher (<i>p</i> < 0.001) in the hydrophobic bilayer only in control male subjects. In conclusion, gender differences were observed in blood oxidative biomarkers, and DPPP value might be suggested as a biomarker predictive of CVD only in men. |
| first_indexed | 2024-03-10T18:15:41Z |
| format | Article |
| id | doaj.art-2a02d3e1629745449a320e9c648b002c |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T18:15:41Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-2a02d3e1629745449a320e9c648b002c2023-11-20T07:41:20ZengMDPI AGBiomedicines2227-90592020-07-018823810.3390/biomedicines8080238Gender-Related Differences in Trimethylamine and Oxidative Blood Biomarkers in Cardiovascular Disease PatientsLaura Bordoni0Donatella Fedeli1Marco Piangerelli2Iwona Pelikant-Malecka3Adrianna Radulska4Joanna J. Samulak5Angelika K. Sawicka6Lukasz Lewicki7Leszek Kalinowski8Robert A. Olek9Rosita Gabbianelli10Unit of Molecular Biology, School of Pharmacy, University of Camerino, 62032 Camerino, ItalyUnit of Molecular Biology, School of Pharmacy, University of Camerino, 62032 Camerino, ItalyComputer Science Division and Mathematics Division, School of Science and Technology, University of Camerino, 62032 Camerino, ItalyDepartment of Medical Laboratory Diagnostics, Medical University of Gdansk, 80-211 Gdansk, PolandDepartment of Medical Laboratory Diagnostics, Medical University of Gdansk, 80-211 Gdansk, PolandDoctoral School for Physical Culture Sciences, 80-336 Gdansk, PolandDoctoral School for Physical Culture Sciences, 80-336 Gdansk, PolandUniversity Center for Cardiology, Gdansk, Debinki 2, 80-211 Gdansk, PolandDepartment of Medical Laboratory Diagnostics, Medical University of Gdansk, 80-211 Gdansk, PolandDepartment of Athletics, Strength and Conditioning, Poznan University of Physical Education, 61-871 Poznan, PolandUnit of Molecular Biology, School of Pharmacy, University of Camerino, 62032 Camerino, ItalyGender differences in the burden of cardiovascular disease (CVD) have been observed worldwide. In this study, plasmatic levels of trimethylamine (TMA) and blood oxidative biomarkers have been evaluated in 358 men (89 controls and 269 CVD patients) and 189 women (64 control and 125 CVD patients). The fluorescence technique was applied to determine erythrocyte membrane fluidity using 1,6-diphenyl-1,3,5-hexatriene (DPH) and Laurdan, while lipid hydroperoxides were assessed by diphenyl−1-pyrenylphosphine (DPPP). Results show that levels of plasmatic TMA were higher in healthy men with respect to healthy women (<i>p</i> = 0.0001). Significantly lower TMA was observed in male CVD patients (0.609 ± 0.104 μM) compared to healthy male controls (0.680 ± 0.118 μM) (<i>p</i> < 0.001), while higher levels of TMA were measured in female CVD patients (0.595 ± 0.115 μM) with respect to female controls (0.529 ± 0.073 μM) (<i>p</i> < 0.001). DPPP was significantly higher in healthy control men than in women (<i>p</i> < 0.001). Male CVD patients displayed a lower value of DPPP (2777 ± 1924) compared to healthy controls (5528 ± 2222) (<i>p</i> < 0.001), while no significant changes were measured in females with or without CVD (<i>p</i> > 0.05). Membrane fluidity was significantly higher (<i>p</i> < 0.001) in the hydrophobic bilayer only in control male subjects. In conclusion, gender differences were observed in blood oxidative biomarkers, and DPPP value might be suggested as a biomarker predictive of CVD only in men.https://www.mdpi.com/2227-9059/8/8/238gendermembrane erythrocytehydroperoxidesbiomarkerDPPPDPH |
| spellingShingle | Laura Bordoni Donatella Fedeli Marco Piangerelli Iwona Pelikant-Malecka Adrianna Radulska Joanna J. Samulak Angelika K. Sawicka Lukasz Lewicki Leszek Kalinowski Robert A. Olek Rosita Gabbianelli Gender-Related Differences in Trimethylamine and Oxidative Blood Biomarkers in Cardiovascular Disease Patients Biomedicines gender membrane erythrocyte hydroperoxides biomarker DPPP DPH |
| title | Gender-Related Differences in Trimethylamine and Oxidative Blood Biomarkers in Cardiovascular Disease Patients |
| title_full | Gender-Related Differences in Trimethylamine and Oxidative Blood Biomarkers in Cardiovascular Disease Patients |
| title_fullStr | Gender-Related Differences in Trimethylamine and Oxidative Blood Biomarkers in Cardiovascular Disease Patients |
| title_full_unstemmed | Gender-Related Differences in Trimethylamine and Oxidative Blood Biomarkers in Cardiovascular Disease Patients |
| title_short | Gender-Related Differences in Trimethylamine and Oxidative Blood Biomarkers in Cardiovascular Disease Patients |
| title_sort | gender related differences in trimethylamine and oxidative blood biomarkers in cardiovascular disease patients |
| topic | gender membrane erythrocyte hydroperoxides biomarker DPPP DPH |
| url | https://www.mdpi.com/2227-9059/8/8/238 |
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