Heparanase Gene Hypomethylation as a Potential Biomarker for Precision Screening of Bladder Cancer
Background: Epigenetics has been playing an increasingly important role in the study of the origin and development of bladder cancer (BC). This study aimed to investigate the correlation between promoter hypomethylation of the heparanase (HPSE) gene and clinicopathologic characteristics of bladder c...
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| Format: | Article |
| Language: | English |
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Hormozgan University of Medical Sciences
2022-07-01
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| Series: | Disease and Diagnosis |
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| Online Access: | https://ddj.hums.ac.ir/PDF/ddj-11-100.pdf |
| _version_ | 1811309973838233600 |
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| author | Bai-sheng Xu Yan-ying Jiang Caizhi Liao Min-bo Yan |
| author_facet | Bai-sheng Xu Yan-ying Jiang Caizhi Liao Min-bo Yan |
| author_sort | Bai-sheng Xu |
| collection | DOAJ |
| description | Background: Epigenetics has been playing an increasingly important role in the study of the origin and development of bladder cancer (BC). This study aimed to investigate the correlation between promoter hypomethylation of the heparanase (HPSE) gene and clinicopathologic characteristics of bladder cancer (BC). Materials and Methods: The promoter hypomethylation profile was evaluated by methylation-specific polymerase chain reaction (PCR) using 27 BC tissue specimens and 15 normal control specimens. The aim was to help decipher the underlying relationship between the clinicopathologic characteristics and the hypomethylation status. Results: Experimental results showed that 16 (59.26%) BC specimens demonstrated the promoter hypomethylation of HPSE, including 2 cases with complete demethylation. For normal control groups, only 3 specimens (20%) indicated hypomethylation (P<0.05). In addition, the occurrence of hypomethylation increased with the metastasis of positive lymph nodes (P<0.05). Importantly, no significant correlation was found between the hypomethylation of HPSE and the profile of patients including gender, age, tumor size, cancer stage, or histologic grade (P>0.05). Conclusion: The promoter hypomethylation of HPSE gene is a common epigenetic event occurring in BC and is positively correlated with a poor prognosis. This study suggested that the promoter hypomethylation could be used as a potential biological marker for the early screening of BC. |
| first_indexed | 2024-04-13T09:50:47Z |
| format | Article |
| id | doaj.art-2a045eb1bcda452398a63ffb2438dc84 |
| institution | Directory Open Access Journal |
| issn | 2717-3232 |
| language | English |
| last_indexed | 2024-04-13T09:50:47Z |
| publishDate | 2022-07-01 |
| publisher | Hormozgan University of Medical Sciences |
| record_format | Article |
| series | Disease and Diagnosis |
| spelling | doaj.art-2a045eb1bcda452398a63ffb2438dc842022-12-22T02:51:36ZengHormozgan University of Medical SciencesDisease and Diagnosis2717-32322022-07-0111310010410.34172/ddj.2022.19ddj-405Heparanase Gene Hypomethylation as a Potential Biomarker for Precision Screening of Bladder CancerBai-sheng Xu0Yan-ying Jiang1Caizhi Liao2Min-bo Yan3The First People’s Hospital Of Xiushui, Jiujiang, Jiangxi Province, 332400, P.R. China.The First People’s Hospital Of Xiushui, Jiujiang, Jiangxi Province, 332400, P.R. China.Creative Biosciences (Guangzhou) Co., Ltd. Guangzhou, 510535, P.R. China.The Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, Guangdong Province, 519000, P.R. China.Background: Epigenetics has been playing an increasingly important role in the study of the origin and development of bladder cancer (BC). This study aimed to investigate the correlation between promoter hypomethylation of the heparanase (HPSE) gene and clinicopathologic characteristics of bladder cancer (BC). Materials and Methods: The promoter hypomethylation profile was evaluated by methylation-specific polymerase chain reaction (PCR) using 27 BC tissue specimens and 15 normal control specimens. The aim was to help decipher the underlying relationship between the clinicopathologic characteristics and the hypomethylation status. Results: Experimental results showed that 16 (59.26%) BC specimens demonstrated the promoter hypomethylation of HPSE, including 2 cases with complete demethylation. For normal control groups, only 3 specimens (20%) indicated hypomethylation (P<0.05). In addition, the occurrence of hypomethylation increased with the metastasis of positive lymph nodes (P<0.05). Importantly, no significant correlation was found between the hypomethylation of HPSE and the profile of patients including gender, age, tumor size, cancer stage, or histologic grade (P>0.05). Conclusion: The promoter hypomethylation of HPSE gene is a common epigenetic event occurring in BC and is positively correlated with a poor prognosis. This study suggested that the promoter hypomethylation could be used as a potential biological marker for the early screening of BC.https://ddj.hums.ac.ir/PDF/ddj-11-100.pdfbladder cancercancer diagnosishypomethylationmethylation-specific polymerase chain reactionheparanase gene |
| spellingShingle | Bai-sheng Xu Yan-ying Jiang Caizhi Liao Min-bo Yan Heparanase Gene Hypomethylation as a Potential Biomarker for Precision Screening of Bladder Cancer Disease and Diagnosis bladder cancer cancer diagnosis hypomethylation methylation-specific polymerase chain reaction heparanase gene |
| title | Heparanase Gene Hypomethylation as a Potential Biomarker for Precision Screening of Bladder Cancer |
| title_full | Heparanase Gene Hypomethylation as a Potential Biomarker for Precision Screening of Bladder Cancer |
| title_fullStr | Heparanase Gene Hypomethylation as a Potential Biomarker for Precision Screening of Bladder Cancer |
| title_full_unstemmed | Heparanase Gene Hypomethylation as a Potential Biomarker for Precision Screening of Bladder Cancer |
| title_short | Heparanase Gene Hypomethylation as a Potential Biomarker for Precision Screening of Bladder Cancer |
| title_sort | heparanase gene hypomethylation as a potential biomarker for precision screening of bladder cancer |
| topic | bladder cancer cancer diagnosis hypomethylation methylation-specific polymerase chain reaction heparanase gene |
| url | https://ddj.hums.ac.ir/PDF/ddj-11-100.pdf |
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