Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album.
Plants with facultative crassulacean acid metabolism (CAM) maximize performance through utilizing C3 or C4 photosynthesis under ideal conditions while temporally switching to CAM under water stress (drought). While genome-scale analyses of constitutive CAM plants suggest that time of day networks ar...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-06-01
|
| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1008209 |
| _version_ | 1818579279913418752 |
|---|---|
| author | Ching Man Wai Sean E Weise Philip Ozersky Todd C Mockler Todd P Michael Robert VanBuren |
| author_facet | Ching Man Wai Sean E Weise Philip Ozersky Todd C Mockler Todd P Michael Robert VanBuren |
| author_sort | Ching Man Wai |
| collection | DOAJ |
| description | Plants with facultative crassulacean acid metabolism (CAM) maximize performance through utilizing C3 or C4 photosynthesis under ideal conditions while temporally switching to CAM under water stress (drought). While genome-scale analyses of constitutive CAM plants suggest that time of day networks are shifted, or phased to the evening compared to C3, little is known for how the shift from C3 to CAM networks is modulated in drought induced CAM. Here we generate a draft genome for the drought-induced CAM-cycling species Sedum album. Through parallel sampling in well-watered (C3) and drought (CAM) conditions, we uncover a massive rewiring of time of day expression and a CAM and stress-specific network. The core circadian genes are expanded in S. album and under CAM induction, core clock genes either change phase or amplitude. While the core clock cis-elements are conserved in S. album, we uncover a set of novel CAM and stress specific cis-elements consistent with our finding of rewired co-expression networks. We identified shared elements between constitutive CAM and CAM-cycling species and expression patterns unique to CAM-cycling S. album. Together these results demonstrate that drought induced CAM-cycling photosynthesis evolved through the mobilization of a stress-specific, time of day network, and not solely the phasing of existing C3 networks. These results will inform efforts to engineer water use efficiency into crop plants for growth on marginal land. |
| first_indexed | 2024-12-16T06:59:11Z |
| format | Article |
| id | doaj.art-2a0816ab12244ce3b75f413af5020425 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-16T06:59:11Z |
| publishDate | 2019-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-2a0816ab12244ce3b75f413af50204252022-12-21T22:40:12ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042019-06-01156e100820910.1371/journal.pgen.1008209Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album.Ching Man WaiSean E WeisePhilip OzerskyTodd C MocklerTodd P MichaelRobert VanBurenPlants with facultative crassulacean acid metabolism (CAM) maximize performance through utilizing C3 or C4 photosynthesis under ideal conditions while temporally switching to CAM under water stress (drought). While genome-scale analyses of constitutive CAM plants suggest that time of day networks are shifted, or phased to the evening compared to C3, little is known for how the shift from C3 to CAM networks is modulated in drought induced CAM. Here we generate a draft genome for the drought-induced CAM-cycling species Sedum album. Through parallel sampling in well-watered (C3) and drought (CAM) conditions, we uncover a massive rewiring of time of day expression and a CAM and stress-specific network. The core circadian genes are expanded in S. album and under CAM induction, core clock genes either change phase or amplitude. While the core clock cis-elements are conserved in S. album, we uncover a set of novel CAM and stress specific cis-elements consistent with our finding of rewired co-expression networks. We identified shared elements between constitutive CAM and CAM-cycling species and expression patterns unique to CAM-cycling S. album. Together these results demonstrate that drought induced CAM-cycling photosynthesis evolved through the mobilization of a stress-specific, time of day network, and not solely the phasing of existing C3 networks. These results will inform efforts to engineer water use efficiency into crop plants for growth on marginal land.https://doi.org/10.1371/journal.pgen.1008209 |
| spellingShingle | Ching Man Wai Sean E Weise Philip Ozersky Todd C Mockler Todd P Michael Robert VanBuren Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album. PLoS Genetics |
| title | Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album. |
| title_full | Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album. |
| title_fullStr | Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album. |
| title_full_unstemmed | Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album. |
| title_short | Time of day and network reprogramming during drought induced CAM photosynthesis in Sedum album. |
| title_sort | time of day and network reprogramming during drought induced cam photosynthesis in sedum album |
| url | https://doi.org/10.1371/journal.pgen.1008209 |
| work_keys_str_mv | AT chingmanwai timeofdayandnetworkreprogrammingduringdroughtinducedcamphotosynthesisinsedumalbum AT seaneweise timeofdayandnetworkreprogrammingduringdroughtinducedcamphotosynthesisinsedumalbum AT philipozersky timeofdayandnetworkreprogrammingduringdroughtinducedcamphotosynthesisinsedumalbum AT toddcmockler timeofdayandnetworkreprogrammingduringdroughtinducedcamphotosynthesisinsedumalbum AT toddpmichael timeofdayandnetworkreprogrammingduringdroughtinducedcamphotosynthesisinsedumalbum AT robertvanburen timeofdayandnetworkreprogrammingduringdroughtinducedcamphotosynthesisinsedumalbum |