A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR)
In the current study, we explored the relationship between melatonin and lactose synthesis in in vivo and in vitro conditions. We found that long-term melatonin feeding to the dairy cows significantly reduced the milk lactose content in a dose dependent manner. This lactose reduction was not associa...
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| Format: | Article |
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PeerJ Inc.
2023-09-01
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| Online Access: | https://peerj.com/articles/15932.pdf |
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| author | Yunjie Liu Songyang Yao Qinggeng Meng Xuening Liu Huigang Han Chunli Kan Tiankun Wang Wenjuan Wei Shujing Li Wenli Yu Zengyuan Zhao Changwang He Guoshi Liu |
| author_facet | Yunjie Liu Songyang Yao Qinggeng Meng Xuening Liu Huigang Han Chunli Kan Tiankun Wang Wenjuan Wei Shujing Li Wenli Yu Zengyuan Zhao Changwang He Guoshi Liu |
| author_sort | Yunjie Liu |
| collection | DOAJ |
| description | In the current study, we explored the relationship between melatonin and lactose synthesis in in vivo and in vitro conditions. We found that long-term melatonin feeding to the dairy cows significantly reduced the milk lactose content in a dose dependent manner. This lactose reduction was not associated with a negative energy balance, since melatonin treatment did not alter the fat, glucose, or protein metabolisms of the cows. To identify the potential molecular mechanisms, the cow’s mammary epithelial cells were cultured for gene expression analysis. The results showed that the effect of melatonin on lactose reduction was mediated by its receptor MT1. MT1 activation downregulated the mRNA expression of the prolactin receptor gene (PRLR), which then suppressed the gene expression of SLC35B1. SLC35B1 is a galactose transporter and is responsible for the transportation of galactose to Golgi apparatus for lactose synthesis. Its suppression reduced the lactose synthesis and the milk lactose content. The discovery of this signal transduction pathway of melatonin on lactose synthesis provides a novel aspect of melatonin’s effect on carbohydrate metabolism in cows and maybe also in other mammals, including humans. |
| first_indexed | 2024-03-09T06:21:37Z |
| format | Article |
| id | doaj.art-2a0976ce8ed8425bb232f465ab1abd6b |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:21:37Z |
| publishDate | 2023-09-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-2a0976ce8ed8425bb232f465ab1abd6b2023-12-03T11:35:32ZengPeerJ Inc.PeerJ2167-83592023-09-0111e1593210.7717/peerj.15932A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR)Yunjie Liu0Songyang Yao1Qinggeng Meng2Xuening Liu3Huigang Han4Chunli Kan5Tiankun Wang6Wenjuan Wei7Shujing Li8Wenli Yu9Zengyuan Zhao10Changwang He11Guoshi Liu12China Agricultural University, Beijing, ChinaChina Agricultural University, Beijing, ChinaBeijing Changping District Nankou Town Agricultural Clothing Center, Beijing, ChinaChina Agricultural University, Beijing, ChinaChina Agricultural University, Beijing, ChinaBeijing Changping District Shisanling Town Agricultural Clothing Center, Beijing, ChinaBeijing Changping District Animal Disease Prevention and Control Center, Beijing, ChinaBeijing Sunlon Livestock Development Co. Ltd., Beijing, ChinaShijiazhuang Tianquan Elite Dairy Co. Ltd., Shijiazhuang, ChinaShijiazhuang Tianquan Elite Dairy Co. Ltd., Shijiazhuang, ChinaShijiazhuang Tianquan Elite Dairy Co. Ltd., Shijiazhuang, ChinaBeijing Sunlon Livestock Development Co. Ltd., Beijing, ChinaChina Agricultural University, Beijing, ChinaIn the current study, we explored the relationship between melatonin and lactose synthesis in in vivo and in vitro conditions. We found that long-term melatonin feeding to the dairy cows significantly reduced the milk lactose content in a dose dependent manner. This lactose reduction was not associated with a negative energy balance, since melatonin treatment did not alter the fat, glucose, or protein metabolisms of the cows. To identify the potential molecular mechanisms, the cow’s mammary epithelial cells were cultured for gene expression analysis. The results showed that the effect of melatonin on lactose reduction was mediated by its receptor MT1. MT1 activation downregulated the mRNA expression of the prolactin receptor gene (PRLR), which then suppressed the gene expression of SLC35B1. SLC35B1 is a galactose transporter and is responsible for the transportation of galactose to Golgi apparatus for lactose synthesis. Its suppression reduced the lactose synthesis and the milk lactose content. The discovery of this signal transduction pathway of melatonin on lactose synthesis provides a novel aspect of melatonin’s effect on carbohydrate metabolism in cows and maybe also in other mammals, including humans.https://peerj.com/articles/15932.pdfDairy cowMelatoninLactose synthesisProlactin |
| spellingShingle | Yunjie Liu Songyang Yao Qinggeng Meng Xuening Liu Huigang Han Chunli Kan Tiankun Wang Wenjuan Wei Shujing Li Wenli Yu Zengyuan Zhao Changwang He Guoshi Liu A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR) PeerJ Dairy cow Melatonin Lactose synthesis Prolactin |
| title | A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR) |
| title_full | A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR) |
| title_fullStr | A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR) |
| title_full_unstemmed | A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR) |
| title_short | A novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 (MT1) and prolactin receptor (PRLR) |
| title_sort | novel signaling transduction pathway of melatonin on lactose synthesis in cows via melatonin receptor 1 mt1 and prolactin receptor prlr |
| topic | Dairy cow Melatonin Lactose synthesis Prolactin |
| url | https://peerj.com/articles/15932.pdf |
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