Feasibility Study of Native Ureolytic Bacteria for Biocementation Towards Coastal Erosion Protection by MICP Method
In recent years, traditional material for coastal erosion protection has become very expensive and not sustainable and eco-friendly for the long term. As an alternative countermeasure, this study focused on a sustainable biological ground improvement technique that can be utilized as an option for i...
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MDPI AG
2019-10-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/9/20/4462 |
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| author | Md Al Imran Shuya Kimura Kazunori Nakashima Niki Evelpidou Satoru Kawasaki |
| author_facet | Md Al Imran Shuya Kimura Kazunori Nakashima Niki Evelpidou Satoru Kawasaki |
| author_sort | Md Al Imran |
| collection | DOAJ |
| description | In recent years, traditional material for coastal erosion protection has become very expensive and not sustainable and eco-friendly for the long term. As an alternative countermeasure, this study focused on a sustainable biological ground improvement technique that can be utilized as an option for improving the mechanical and geotechnical engineering properties of soil by the microbially induced carbonate precipitation (MICP) technique considering native ureolytic bacteria. To protect coastal erosion, an innovative and sustainable strategy was proposed in this study by means of combing geotube and the MICP method. For a successful sand solidification, the urease activity, environmental factors, urease distribution, and calcite precipitation trend, among others, have been investigated using the isolated native strains. Our results revealed that urease activity of the identified strains denoted as G1 (<i>Micrococcus</i> sp.), G2 (<i>Pseudoalteromonas</i> sp.), and G3 (<i>Virgibacillus</i> sp.) relied on environment-specific parameters and, additionally, urease was not discharged in the culture solution but would discharge in and/or on the bacterial cell, and the fluid of the cells showed urease activity. Moreover, we successfully obtained solidified sand bearing UCS (Unconfined Compressive Strength) up to 1.8 MPa. We also proposed a novel sustainable approach for field implementation in a combination of geotube and MICP for coastal erosion protection that is cheaper, energy-saving, eco-friendly, and sustainable for Mediterranean countries, as well as for bio-mediated soil improvement. |
| first_indexed | 2024-04-14T06:22:08Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-04-14T06:22:08Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-2a0b516695174acda25411a6bc340a932022-12-22T02:07:59ZengMDPI AGApplied Sciences2076-34172019-10-01920446210.3390/app9204462app9204462Feasibility Study of Native Ureolytic Bacteria for Biocementation Towards Coastal Erosion Protection by MICP MethodMd Al Imran0Shuya Kimura1Kazunori Nakashima2Niki Evelpidou3Satoru Kawasaki4Division of Sustainable Resources Engineering, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, JapanDivision of Sustainable Resources Engineering, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, JapanDivision of Sustainable Resources Engineering, Faculty of Engineering, Hokkaido University, Sapporo 060-8628, JapanSchool of Science, Faculty of Geology and Geoenvironment, National and Kapodistrian University of Athens, 15784 Athens, GreeceDivision of Sustainable Resources Engineering, Faculty of Engineering, Hokkaido University, Sapporo 060-8628, JapanIn recent years, traditional material for coastal erosion protection has become very expensive and not sustainable and eco-friendly for the long term. As an alternative countermeasure, this study focused on a sustainable biological ground improvement technique that can be utilized as an option for improving the mechanical and geotechnical engineering properties of soil by the microbially induced carbonate precipitation (MICP) technique considering native ureolytic bacteria. To protect coastal erosion, an innovative and sustainable strategy was proposed in this study by means of combing geotube and the MICP method. For a successful sand solidification, the urease activity, environmental factors, urease distribution, and calcite precipitation trend, among others, have been investigated using the isolated native strains. Our results revealed that urease activity of the identified strains denoted as G1 (<i>Micrococcus</i> sp.), G2 (<i>Pseudoalteromonas</i> sp.), and G3 (<i>Virgibacillus</i> sp.) relied on environment-specific parameters and, additionally, urease was not discharged in the culture solution but would discharge in and/or on the bacterial cell, and the fluid of the cells showed urease activity. Moreover, we successfully obtained solidified sand bearing UCS (Unconfined Compressive Strength) up to 1.8 MPa. We also proposed a novel sustainable approach for field implementation in a combination of geotube and MICP for coastal erosion protection that is cheaper, energy-saving, eco-friendly, and sustainable for Mediterranean countries, as well as for bio-mediated soil improvement.https://www.mdpi.com/2076-3417/9/20/4462microbially induced carbonate precipitationureolytic bacteriaurease activitybiomineralizationcoastal erosion protectionartificial beachrock |
| spellingShingle | Md Al Imran Shuya Kimura Kazunori Nakashima Niki Evelpidou Satoru Kawasaki Feasibility Study of Native Ureolytic Bacteria for Biocementation Towards Coastal Erosion Protection by MICP Method Applied Sciences microbially induced carbonate precipitation ureolytic bacteria urease activity biomineralization coastal erosion protection artificial beachrock |
| title | Feasibility Study of Native Ureolytic Bacteria for Biocementation Towards Coastal Erosion Protection by MICP Method |
| title_full | Feasibility Study of Native Ureolytic Bacteria for Biocementation Towards Coastal Erosion Protection by MICP Method |
| title_fullStr | Feasibility Study of Native Ureolytic Bacteria for Biocementation Towards Coastal Erosion Protection by MICP Method |
| title_full_unstemmed | Feasibility Study of Native Ureolytic Bacteria for Biocementation Towards Coastal Erosion Protection by MICP Method |
| title_short | Feasibility Study of Native Ureolytic Bacteria for Biocementation Towards Coastal Erosion Protection by MICP Method |
| title_sort | feasibility study of native ureolytic bacteria for biocementation towards coastal erosion protection by micp method |
| topic | microbially induced carbonate precipitation ureolytic bacteria urease activity biomineralization coastal erosion protection artificial beachrock |
| url | https://www.mdpi.com/2076-3417/9/20/4462 |
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