Long Non-Coding RNA <i>GDAR</i> Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes
Short-term dietary supplementation of ewes during the luteal phase can increase fertility, most probably by stimulating glucose uptake by the follicles. However, the molecular mechanism of glucose regulation of follicular development has not yet been clarified, especially the further study of long n...
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MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
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| author | Yong Wang Yunxia Guo Chunhui Duan Ruochen Yang Lechao Zhang Yueqin Liu Yingjie Zhang |
| author_facet | Yong Wang Yunxia Guo Chunhui Duan Ruochen Yang Lechao Zhang Yueqin Liu Yingjie Zhang |
| author_sort | Yong Wang |
| collection | DOAJ |
| description | Short-term dietary supplementation of ewes during the luteal phase can increase fertility, most probably by stimulating glucose uptake by the follicles. However, the molecular mechanism of glucose regulation of follicular development has not yet been clarified, especially the further study of long non-coding RNA (lncRNA) in determining fertility during follicular development. We generated granulosa cell (GC) models of different doses of glucose (0, 2.1, 4.2, 8.4, 16.8 and 33.6 mM), and observed that the highest cell viability was recorded in the 8.4 mM group and the highest apoptosis rates were recorded in the 33.6 mM group. Therefore, a control group (<i>n</i> = 3, 0 mM glucose), a low glucose group (<i>n</i> = 3, add 8.4 mM glucose), and a high glucose group (<i>n</i> = 3, add 33.6 mM glucose) of GCs were created for next whole genomic RNA sequencing. In total, 18,172 novel lncRNAs and 510 annotated lncRNAs were identified in the GCs samples. Gene Ontology indicated that differentially expressed lncRNAs associated with cell apoptosis were highly enriched. Kyoto Encyclopedia of Genes and Genomes enrichment analysis of lncRNA target genes found that the apoptosis pathway and the p53 signaling pathway were both enriched. Furthermore, we focused on the function of a lnc<i>GDAR</i> and verified that lncG<i>DAR</i> could influence cell apoptosis in GC development through affecting the mRNA and protein expression of apoptosis-related markers. These results provide the basis for further study of the lncRNA regulation mechanism in nutrition on female fertility. |
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| last_indexed | 2024-03-10T04:03:06Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2a0cc16ba00147dc8617e2e7e312eea02023-11-23T08:28:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-01239518310.3390/ijms23095183Long Non-Coding RNA <i>GDAR</i> Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related GenesYong Wang0Yunxia Guo1Chunhui Duan2Ruochen Yang3Lechao Zhang4Yueqin Liu5Yingjie Zhang6College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Science, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaShort-term dietary supplementation of ewes during the luteal phase can increase fertility, most probably by stimulating glucose uptake by the follicles. However, the molecular mechanism of glucose regulation of follicular development has not yet been clarified, especially the further study of long non-coding RNA (lncRNA) in determining fertility during follicular development. We generated granulosa cell (GC) models of different doses of glucose (0, 2.1, 4.2, 8.4, 16.8 and 33.6 mM), and observed that the highest cell viability was recorded in the 8.4 mM group and the highest apoptosis rates were recorded in the 33.6 mM group. Therefore, a control group (<i>n</i> = 3, 0 mM glucose), a low glucose group (<i>n</i> = 3, add 8.4 mM glucose), and a high glucose group (<i>n</i> = 3, add 33.6 mM glucose) of GCs were created for next whole genomic RNA sequencing. In total, 18,172 novel lncRNAs and 510 annotated lncRNAs were identified in the GCs samples. Gene Ontology indicated that differentially expressed lncRNAs associated with cell apoptosis were highly enriched. Kyoto Encyclopedia of Genes and Genomes enrichment analysis of lncRNA target genes found that the apoptosis pathway and the p53 signaling pathway were both enriched. Furthermore, we focused on the function of a lnc<i>GDAR</i> and verified that lncG<i>DAR</i> could influence cell apoptosis in GC development through affecting the mRNA and protein expression of apoptosis-related markers. These results provide the basis for further study of the lncRNA regulation mechanism in nutrition on female fertility.https://www.mdpi.com/1422-0067/23/9/5183granulosa cellglucoseapoptosislong non-coding RNA |
| spellingShingle | Yong Wang Yunxia Guo Chunhui Duan Ruochen Yang Lechao Zhang Yueqin Liu Yingjie Zhang Long Non-Coding RNA <i>GDAR</i> Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes International Journal of Molecular Sciences granulosa cell glucose apoptosis long non-coding RNA |
| title | Long Non-Coding RNA <i>GDAR</i> Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes |
| title_full | Long Non-Coding RNA <i>GDAR</i> Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes |
| title_fullStr | Long Non-Coding RNA <i>GDAR</i> Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes |
| title_full_unstemmed | Long Non-Coding RNA <i>GDAR</i> Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes |
| title_short | Long Non-Coding RNA <i>GDAR</i> Regulates Ovine Granulosa Cells Apoptosis by Affecting the Expression of Apoptosis-Related Genes |
| title_sort | long non coding rna i gdar i regulates ovine granulosa cells apoptosis by affecting the expression of apoptosis related genes |
| topic | granulosa cell glucose apoptosis long non-coding RNA |
| url | https://www.mdpi.com/1422-0067/23/9/5183 |
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