Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for <em>Staphylococcus Aureus</em>
Although nanomaterials are used in many fields, little is known about the fundamental interactions between nanomaterials and microorganisms. To test antimicrobial properties and retention ability, 13 electrospun polyamide (PA) nanomaterials with different morphology and functionalization with variou...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/2/480 |
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| author | Simona Lencova Kamila Zdenkova Vera Jencova Katerina Demnerova Klara Zemanova Radka Kolackova Kristyna Hozdova Hana Stiborova |
| author_facet | Simona Lencova Kamila Zdenkova Vera Jencova Katerina Demnerova Klara Zemanova Radka Kolackova Kristyna Hozdova Hana Stiborova |
| author_sort | Simona Lencova |
| collection | DOAJ |
| description | Although nanomaterials are used in many fields, little is known about the fundamental interactions between nanomaterials and microorganisms. To test antimicrobial properties and retention ability, 13 electrospun polyamide (PA) nanomaterials with different morphology and functionalization with various concentrations of AgNO<sub>3</sub> and chlorhexidine (CHX) were analyzed. <i>Staphylococcus aureus</i> CCM 4516 was used to verify the designed nanomaterials’ inhibition and permeability assays. All functionalized PAs suppressed bacterial growth, and the most effective antimicrobial nanomaterial was evaluated to be PA 12% with 4.0 wt% CHX (inhibition zones: 2.9 ± 0.2 mm; log<sub>10</sub> suppression: 8.9 ± 0.0; inhibitory rate: 100.0%). Furthermore, the long-term stability of all functionalized PAs was tested. These nanomaterials can be stored at least nine months after their preparation without losing their antibacterial effect. A filtration apparatus was constructed for testing the retention of PAs. All of the PAs effectively retained the filtered bacteria with log<sub>10</sub> removal of 3.3–6.8 and a retention rate of 96.7–100.0%. Surface density significantly influenced the retention efficiency of PAs (<i>p</i> ≤ 0.01), while the effect of fiber diameter was not confirmed (<i>p</i> ≥ 0.05). Due to their stability, retention, and antimicrobial properties, they can serve as a model for medical or filtration applications. |
| first_indexed | 2024-03-09T00:53:17Z |
| format | Article |
| id | doaj.art-2a0cd266a32446a4927d48f594e05987 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T00:53:17Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-2a0cd266a32446a4927d48f594e059872023-12-11T17:01:09ZengMDPI AGNanomaterials2079-49912021-02-0111248010.3390/nano11020480Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for <em>Staphylococcus Aureus</em>Simona Lencova0Kamila Zdenkova1Vera Jencova2Katerina Demnerova3Klara Zemanova4Radka Kolackova5Kristyna Hozdova6Hana Stiborova7Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Technicka 3, 16628 Prague 6, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Technicka 3, 16628 Prague 6, Czech RepublicFaculty of Science, Humanities and Education, Technical University of Liberec, Studentska 1402/2, 461 17 Liberec 1, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Technicka 3, 16628 Prague 6, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Technicka 3, 16628 Prague 6, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Technicka 3, 16628 Prague 6, Czech RepublicElmarco Ltd., Svarovska 621, 460 01 Liberec, Czech RepublicDepartment of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Technicka 3, 16628 Prague 6, Czech RepublicAlthough nanomaterials are used in many fields, little is known about the fundamental interactions between nanomaterials and microorganisms. To test antimicrobial properties and retention ability, 13 electrospun polyamide (PA) nanomaterials with different morphology and functionalization with various concentrations of AgNO<sub>3</sub> and chlorhexidine (CHX) were analyzed. <i>Staphylococcus aureus</i> CCM 4516 was used to verify the designed nanomaterials’ inhibition and permeability assays. All functionalized PAs suppressed bacterial growth, and the most effective antimicrobial nanomaterial was evaluated to be PA 12% with 4.0 wt% CHX (inhibition zones: 2.9 ± 0.2 mm; log<sub>10</sub> suppression: 8.9 ± 0.0; inhibitory rate: 100.0%). Furthermore, the long-term stability of all functionalized PAs was tested. These nanomaterials can be stored at least nine months after their preparation without losing their antibacterial effect. A filtration apparatus was constructed for testing the retention of PAs. All of the PAs effectively retained the filtered bacteria with log<sub>10</sub> removal of 3.3–6.8 and a retention rate of 96.7–100.0%. Surface density significantly influenced the retention efficiency of PAs (<i>p</i> ≤ 0.01), while the effect of fiber diameter was not confirmed (<i>p</i> ≥ 0.05). Due to their stability, retention, and antimicrobial properties, they can serve as a model for medical or filtration applications.https://www.mdpi.com/2079-4991/11/2/480polyamidenanofiberpermeabilityfiltration apparatusantimicrobial activity<i>Staphylococcus aureus</i> |
| spellingShingle | Simona Lencova Kamila Zdenkova Vera Jencova Katerina Demnerova Klara Zemanova Radka Kolackova Kristyna Hozdova Hana Stiborova Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for <em>Staphylococcus Aureus</em> Nanomaterials polyamide nanofiber permeability filtration apparatus antimicrobial activity <i>Staphylococcus aureus</i> |
| title | Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for <em>Staphylococcus Aureus</em> |
| title_full | Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for <em>Staphylococcus Aureus</em> |
| title_fullStr | Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for <em>Staphylococcus Aureus</em> |
| title_full_unstemmed | Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for <em>Staphylococcus Aureus</em> |
| title_short | Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for <em>Staphylococcus Aureus</em> |
| title_sort | benefits of polyamide nanofibrous materials antibacterial activity and retention ability for em staphylococcus aureus em |
| topic | polyamide nanofiber permeability filtration apparatus antimicrobial activity <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/2079-4991/11/2/480 |
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