A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of <i>Anoplocephala perfoliata</i> Infected and Non-Infected Horses: A Pilot Study
<i>Anoplocephala perfoliata</i> is a common equine tapeworm associated with an increased risk of colic (abdominal pain) in horses. Identification of parasite and intestinal microbiota interactions have consequences for understanding the mechanisms behind parasite-associated colic and pot...
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MDPI AG
2021-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/3/755 |
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| author | Rachael Slater Alessandra Frau Jane Hodgkinson Debra Archer Chris Probert |
| author_facet | Rachael Slater Alessandra Frau Jane Hodgkinson Debra Archer Chris Probert |
| author_sort | Rachael Slater |
| collection | DOAJ |
| description | <i>Anoplocephala perfoliata</i> is a common equine tapeworm associated with an increased risk of colic (abdominal pain) in horses. Identification of parasite and intestinal microbiota interactions have consequences for understanding the mechanisms behind parasite-associated colic and potential new methods for parasite control. <i>A. perfoliata</i> was diagnosed by counting of worms in the caecum post-mortem. Bacterial DNA was extracted from colonic contents and sequenced targeting of the 16S rRNA gene (V4 region). The volatile organic compound (VOC) metabolome of colonic contents was characterised using gas chromatography mass spectrometry. Bacterial diversity (alpha and beta) was similar between tapeworm infected and non-infected controls. Some compositional differences were apparent with down-regulation of operational taxonomic units (OTUs) belonging to the symbiotic families of Ruminococcaceae and Lachnospiraceae in the tapeworm-infected group. Overall tapeworm burden accounted for 7–8% of variation in the VOC profile (permutational multivariate analysis of variance). Integration of bacterial OTUs and VOCs demonstrated moderate to strong correlations indicating the potential of VOCs as markers for bacterial OTUs in equine colonic contents. This study has shown potential differences in the intestinal microbiome and metabolome of <i>A. perfoliata</i> infected and non-infected horses. This pilot study did not control for extrinsic factors including diet, disease history and stage of infection. |
| first_indexed | 2024-03-10T13:23:56Z |
| format | Article |
| id | doaj.art-2a0e298f8d59424eb373be2b28140be4 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:23:56Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-2a0e298f8d59424eb373be2b28140be42023-11-21T09:48:57ZengMDPI AGAnimals2076-26152021-03-0111375510.3390/ani11030755A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of <i>Anoplocephala perfoliata</i> Infected and Non-Infected Horses: A Pilot StudyRachael Slater0Alessandra Frau1Jane Hodgkinson2Debra Archer3Chris Probert4Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 3GE, UKInstitute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 3GE, UKInstitute of Infection, Veterinary and Ecological Science, University of Liverpool, Leahurst Campus, Chester High Road, Wirral CH64 7TE, UKInstitute of Infection, Veterinary and Ecological Science, University of Liverpool, Leahurst Campus, Chester High Road, Wirral CH64 7TE, UKInstitute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 3GE, UK<i>Anoplocephala perfoliata</i> is a common equine tapeworm associated with an increased risk of colic (abdominal pain) in horses. Identification of parasite and intestinal microbiota interactions have consequences for understanding the mechanisms behind parasite-associated colic and potential new methods for parasite control. <i>A. perfoliata</i> was diagnosed by counting of worms in the caecum post-mortem. Bacterial DNA was extracted from colonic contents and sequenced targeting of the 16S rRNA gene (V4 region). The volatile organic compound (VOC) metabolome of colonic contents was characterised using gas chromatography mass spectrometry. Bacterial diversity (alpha and beta) was similar between tapeworm infected and non-infected controls. Some compositional differences were apparent with down-regulation of operational taxonomic units (OTUs) belonging to the symbiotic families of Ruminococcaceae and Lachnospiraceae in the tapeworm-infected group. Overall tapeworm burden accounted for 7–8% of variation in the VOC profile (permutational multivariate analysis of variance). Integration of bacterial OTUs and VOCs demonstrated moderate to strong correlations indicating the potential of VOCs as markers for bacterial OTUs in equine colonic contents. This study has shown potential differences in the intestinal microbiome and metabolome of <i>A. perfoliata</i> infected and non-infected horses. This pilot study did not control for extrinsic factors including diet, disease history and stage of infection.https://www.mdpi.com/2076-2615/11/3/755anoplocephala perfoliataequinegut microbiomevolatile organic compounds (VOCs)omics integration |
| spellingShingle | Rachael Slater Alessandra Frau Jane Hodgkinson Debra Archer Chris Probert A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of <i>Anoplocephala perfoliata</i> Infected and Non-Infected Horses: A Pilot Study Animals anoplocephala perfoliata equine gut microbiome volatile organic compounds (VOCs) omics integration |
| title | A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of <i>Anoplocephala perfoliata</i> Infected and Non-Infected Horses: A Pilot Study |
| title_full | A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of <i>Anoplocephala perfoliata</i> Infected and Non-Infected Horses: A Pilot Study |
| title_fullStr | A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of <i>Anoplocephala perfoliata</i> Infected and Non-Infected Horses: A Pilot Study |
| title_full_unstemmed | A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of <i>Anoplocephala perfoliata</i> Infected and Non-Infected Horses: A Pilot Study |
| title_short | A Comparison of the Colonic Microbiome and Volatile Organic Compound Metabolome of <i>Anoplocephala perfoliata</i> Infected and Non-Infected Horses: A Pilot Study |
| title_sort | comparison of the colonic microbiome and volatile organic compound metabolome of i anoplocephala perfoliata i infected and non infected horses a pilot study |
| topic | anoplocephala perfoliata equine gut microbiome volatile organic compounds (VOCs) omics integration |
| url | https://www.mdpi.com/2076-2615/11/3/755 |
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