Protection of retinal ganglion cells against optic nerve injury by induction of ischemic preconditioning
AIM: To explore if ischemic preconditioning (IPC) can enhance the survival of retinal ganglion cells (RGCs) after optic nerve axotomy. METHODS: Twenty-four hours prior to retinal ischemia 60min or axotomy, IPC was applied for ten minutes in groups of (n=72) animals. The survival of RGCs, the cellul...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2017-06-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://www.ijo.cn/en_publish/2017/6/20170605.pdf |
| _version_ | 1818512445785767936 |
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| author | Xia Liu Jiu-Ping Liang Ou Sha Song-Juan Wang Heng-Guo Li Eric Y.P. Cho |
| author_facet | Xia Liu Jiu-Ping Liang Ou Sha Song-Juan Wang Heng-Guo Li Eric Y.P. Cho |
| author_sort | Xia Liu |
| collection | DOAJ |
| description | AIM: To explore if ischemic preconditioning (IPC) can enhance the survival of retinal ganglion cells (RGCs) after optic nerve axotomy.
METHODS: Twenty-four hours prior to retinal ischemia 60min or axotomy, IPC was applied for ten minutes in groups of (n=72) animals. The survival of RGCs, the cellular expression of heat shock protein 27 (HSP27) and heat shock protein 70 (HSP70) and the numbers of retinal microglia in the different groups were quantified at 7 and 14d post-injury. The cellular expression of HSP27 and HSP70 and changes in the numbers of retinal microglia were quantified to detect the possible mechanism of the protection of the IPC.
RESULTS: Ten minutes of IPC promoted RGC survival in both the optic nerve injury (IPC-ONT) and the retinal ischemia 60min (IPC-IR60) groups, examined at 7d and 14d post-injury. Microglial proliferation showed little correlation with the extent of benefit effects of IPC on the rescue of RGCs. The number of HSP27-positive RGCs was significantly higher in the IPC-ONT group than in the sham IPC-ONT group, although the percentage of HSP27-positive RGCs did not significantly differ between groups. For the IPC-IR60 group, neither the number nor the percentage of the HSP27-positive RGCs differed significantly between the IPC and the sham-operated groups. The number of HSP70-positive RGCs was significantly higher for both the IPC-ONT and the IPC-IR60 experimental groups, but the percentages did not differ.
CONCLUSION: The induction of IPC enhances the survival of RGCs against both axotomy and retinal ischemia. |
| first_indexed | 2024-12-10T23:47:03Z |
| format | Article |
| id | doaj.art-2a13abb5560b4520b82c519ab65bbb5d |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-10T23:47:03Z |
| publishDate | 2017-06-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-2a13abb5560b4520b82c519ab65bbb5d2022-12-22T01:28:53ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982017-06-0110685486110.18240/ijo.2017.06.05Protection of retinal ganglion cells against optic nerve injury by induction of ischemic preconditioningXia Liu0Jiu-Ping Liang1Ou Sha2Song-Juan Wang3Heng-Guo Li4Eric Y.P. Cho5Medical Imaging Center, the First Affiliated Clinical Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China; School of Biomedical Sciences, the Chinese University of Hong Kong, Shatin, Hong Kong 999077, ChinaMedical Imaging Center, the First Affiliated Clinical Hospital of Jinan University, Guangzhou 510632, Guangdong Province, ChinaDepartment of Preclinical Medicine, School of Medicine, Shenzhen University, Shenzhen 518060, Guangdong Province, ChinaShiyan People’s Hospital of Baoan District, Shenzhen 518108, Guangdong Province, ChinaMedical Imaging Center, the First Affiliated Clinical Hospital of Jinan University, Guangzhou 510632, Guangdong Province, ChinaSchool of Biomedical Sciences, the Chinese University of Hong Kong, Shatin, Hong Kong 999077, ChinaAIM: To explore if ischemic preconditioning (IPC) can enhance the survival of retinal ganglion cells (RGCs) after optic nerve axotomy. METHODS: Twenty-four hours prior to retinal ischemia 60min or axotomy, IPC was applied for ten minutes in groups of (n=72) animals. The survival of RGCs, the cellular expression of heat shock protein 27 (HSP27) and heat shock protein 70 (HSP70) and the numbers of retinal microglia in the different groups were quantified at 7 and 14d post-injury. The cellular expression of HSP27 and HSP70 and changes in the numbers of retinal microglia were quantified to detect the possible mechanism of the protection of the IPC. RESULTS: Ten minutes of IPC promoted RGC survival in both the optic nerve injury (IPC-ONT) and the retinal ischemia 60min (IPC-IR60) groups, examined at 7d and 14d post-injury. Microglial proliferation showed little correlation with the extent of benefit effects of IPC on the rescue of RGCs. The number of HSP27-positive RGCs was significantly higher in the IPC-ONT group than in the sham IPC-ONT group, although the percentage of HSP27-positive RGCs did not significantly differ between groups. For the IPC-IR60 group, neither the number nor the percentage of the HSP27-positive RGCs differed significantly between the IPC and the sham-operated groups. The number of HSP70-positive RGCs was significantly higher for both the IPC-ONT and the IPC-IR60 experimental groups, but the percentages did not differ. CONCLUSION: The induction of IPC enhances the survival of RGCs against both axotomy and retinal ischemia.http://www.ijo.cn/en_publish/2017/6/20170605.pdf861ischemic preconditioningretinal ganglion cellsaxotomyretinal ischemia/reperfusionheat shock protein 27 and 70 |
| spellingShingle | Xia Liu Jiu-Ping Liang Ou Sha Song-Juan Wang Heng-Guo Li Eric Y.P. Cho Protection of retinal ganglion cells against optic nerve injury by induction of ischemic preconditioning International Journal of Ophthalmology 861 ischemic preconditioning retinal ganglion cells axotomy retinal ischemia/reperfusion heat shock protein 27 and 70 |
| title | Protection of retinal ganglion cells against optic nerve injury by induction of ischemic preconditioning |
| title_full | Protection of retinal ganglion cells against optic nerve injury by induction of ischemic preconditioning |
| title_fullStr | Protection of retinal ganglion cells against optic nerve injury by induction of ischemic preconditioning |
| title_full_unstemmed | Protection of retinal ganglion cells against optic nerve injury by induction of ischemic preconditioning |
| title_short | Protection of retinal ganglion cells against optic nerve injury by induction of ischemic preconditioning |
| title_sort | protection of retinal ganglion cells against optic nerve injury by induction of ischemic preconditioning |
| topic | 861 ischemic preconditioning retinal ganglion cells axotomy retinal ischemia/reperfusion heat shock protein 27 and 70 |
| url | http://www.ijo.cn/en_publish/2017/6/20170605.pdf |
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