Herbal Vitamin C Prevents DNA Oxidation and Modifies the Metabolomic Water Profile of Tilapia (<i>Oreochromis</i> spp.)
This experiment aimed to evaluate the effects of herbal vitamin C at different levels on tilapia (<i>Oreochromis</i> spp.) growth, potential DNA damage, and the metabolomic profile of water effluent. Forty-five tilapias were housed in separate plastic tanks (80 L), and these were randoml...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/8/1243 |
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| author | Moisés Villanueva Guillermo Espinosa-Reyes Rogelio Flores-Ramirez Angel Natanael Rojas-Velazquez Juan Carlos García López Anayeli Vazquez-Valladolid José Alejandro Roque-Jimenez German D. Mendoza-Martinez Pedro A. Hernandez-Garcia Monika Palacios-Martinez Alfonso J. Chay-Canul Héctor A. Lee-Rangel |
| author_facet | Moisés Villanueva Guillermo Espinosa-Reyes Rogelio Flores-Ramirez Angel Natanael Rojas-Velazquez Juan Carlos García López Anayeli Vazquez-Valladolid José Alejandro Roque-Jimenez German D. Mendoza-Martinez Pedro A. Hernandez-Garcia Monika Palacios-Martinez Alfonso J. Chay-Canul Héctor A. Lee-Rangel |
| author_sort | Moisés Villanueva |
| collection | DOAJ |
| description | This experiment aimed to evaluate the effects of herbal vitamin C at different levels on tilapia (<i>Oreochromis</i> spp.) growth, potential DNA damage, and the metabolomic profile of water effluent. Forty-five tilapias were housed in separate plastic tanks (80 L), and these were randomly assigned to three treatments: (a) a commercial diet (CD) only; (Nutripec Purina<sup>®</sup>); (b) the commercial diet plus 250 mg of herbal vitamin C (HVC)/kg (CD250); and (c) the commercial diet plus 500 mg of HVC/kg (CD500). Biometric measurements were taken each week, blood samples were collected from the caudal vein on the final day, and water effluent was taken each week and immediately frozen (−80 °C) until further analysis (gas chromatography/mass spectrometry (GC/MS) systems). Data were completely randomized with a 2 × 2 factorial arrangement of treatments. Upon including herbal vitamin C, the final BW (<i>p</i> = 0.05) and BWG (<i>p</i> = 0.06) increased linearly. Herbal vitamin C decreases DNA damage (<i>p</i> ≥ 0.05). PLS-DA showed a 41.6% variation between treatments in the water samples. Fifteen metabolites had the best association between treatments, with a stronger correlation with CD500. Herbal vitamin C could improve fish performance, prevent DNA damage, and influence changes in the metabolomic profile of the water. |
| first_indexed | 2024-03-09T04:11:25Z |
| format | Article |
| id | doaj.art-2a147bda24804e8cbc2de97fbd105dec |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T04:11:25Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-2a147bda24804e8cbc2de97fbd105dec2023-12-03T13:59:01ZengMDPI AGLife2075-17292022-08-01128124310.3390/life12081243Herbal Vitamin C Prevents DNA Oxidation and Modifies the Metabolomic Water Profile of Tilapia (<i>Oreochromis</i> spp.)Moisés Villanueva0Guillermo Espinosa-Reyes1Rogelio Flores-Ramirez2Angel Natanael Rojas-Velazquez3Juan Carlos García López4Anayeli Vazquez-Valladolid5José Alejandro Roque-Jimenez6German D. Mendoza-Martinez7Pedro A. Hernandez-Garcia8Monika Palacios-Martinez9Alfonso J. Chay-Canul10Héctor A. Lee-Rangel11Facultad de Agronomía y Veterinaria, Centro de Biociencias, Instituto de Investigaciones en Zonas Desérticas, Universidad Autónoma de San Luis Potosí, S.L.P., Soledad de Graciano Sánchez 78000, MexicoFacultad de Medicina—CIACYT, Centro de Investigación Aplicada en Ambiente y Salud, Universidad Autónoma de San Luis Potosí, Lomas Segunda Sección, San Luis Potosí 78210, MexicoFacultad de Medicina—CIACYT, Centro de Investigación Aplicada en Ambiente y Salud, Universidad Autónoma de San Luis Potosí, Lomas Segunda Sección, San Luis Potosí 78210, MexicoFacultad de Agronomía y Veterinaria, Centro de Biociencias, Instituto de Investigaciones en Zonas Desérticas, Universidad Autónoma de San Luis Potosí, S.L.P., Soledad de Graciano Sánchez 78000, MexicoFacultad de Agronomía y Veterinaria, Centro de Biociencias, Instituto de Investigaciones en Zonas Desérticas, Universidad Autónoma de San Luis Potosí, S.L.P., Soledad de Graciano Sánchez 78000, MexicoFacultad de Medicina—CIACYT, Centro de Investigación Aplicada en Ambiente y Salud, Universidad Autónoma de San Luis Potosí, Lomas Segunda Sección, San Luis Potosí 78210, MexicoDepartamento de Producción Animal, Universidad Autónoma Metropolitana—Xochimilco, CDMX, Mexico City 04960, MexicoDepartamento de Producción Animal, Universidad Autónoma Metropolitana—Xochimilco, CDMX, Mexico City 04960, MexicoCentro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Carretera Federal Amecameca-Ayapango km 2.5, Amecameca de Juárez 56900, MexicoDepartamento de Producción Animal, Universidad Autónoma Metropolitana—Xochimilco, CDMX, Mexico City 04960, MexicoDivisión Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Teapa, km 25, R/A. La Huasteca 2ª Sección, Villahermosa 86280, MexicoFacultad de Agronomía y Veterinaria, Centro de Biociencias, Instituto de Investigaciones en Zonas Desérticas, Universidad Autónoma de San Luis Potosí, S.L.P., Soledad de Graciano Sánchez 78000, MexicoThis experiment aimed to evaluate the effects of herbal vitamin C at different levels on tilapia (<i>Oreochromis</i> spp.) growth, potential DNA damage, and the metabolomic profile of water effluent. Forty-five tilapias were housed in separate plastic tanks (80 L), and these were randomly assigned to three treatments: (a) a commercial diet (CD) only; (Nutripec Purina<sup>®</sup>); (b) the commercial diet plus 250 mg of herbal vitamin C (HVC)/kg (CD250); and (c) the commercial diet plus 500 mg of HVC/kg (CD500). Biometric measurements were taken each week, blood samples were collected from the caudal vein on the final day, and water effluent was taken each week and immediately frozen (−80 °C) until further analysis (gas chromatography/mass spectrometry (GC/MS) systems). Data were completely randomized with a 2 × 2 factorial arrangement of treatments. Upon including herbal vitamin C, the final BW (<i>p</i> = 0.05) and BWG (<i>p</i> = 0.06) increased linearly. Herbal vitamin C decreases DNA damage (<i>p</i> ≥ 0.05). PLS-DA showed a 41.6% variation between treatments in the water samples. Fifteen metabolites had the best association between treatments, with a stronger correlation with CD500. Herbal vitamin C could improve fish performance, prevent DNA damage, and influence changes in the metabolomic profile of the water.https://www.mdpi.com/2075-1729/12/8/1243herbal vitamin CDNA damagetilapia |
| spellingShingle | Moisés Villanueva Guillermo Espinosa-Reyes Rogelio Flores-Ramirez Angel Natanael Rojas-Velazquez Juan Carlos García López Anayeli Vazquez-Valladolid José Alejandro Roque-Jimenez German D. Mendoza-Martinez Pedro A. Hernandez-Garcia Monika Palacios-Martinez Alfonso J. Chay-Canul Héctor A. Lee-Rangel Herbal Vitamin C Prevents DNA Oxidation and Modifies the Metabolomic Water Profile of Tilapia (<i>Oreochromis</i> spp.) Life herbal vitamin C DNA damage tilapia |
| title | Herbal Vitamin C Prevents DNA Oxidation and Modifies the Metabolomic Water Profile of Tilapia (<i>Oreochromis</i> spp.) |
| title_full | Herbal Vitamin C Prevents DNA Oxidation and Modifies the Metabolomic Water Profile of Tilapia (<i>Oreochromis</i> spp.) |
| title_fullStr | Herbal Vitamin C Prevents DNA Oxidation and Modifies the Metabolomic Water Profile of Tilapia (<i>Oreochromis</i> spp.) |
| title_full_unstemmed | Herbal Vitamin C Prevents DNA Oxidation and Modifies the Metabolomic Water Profile of Tilapia (<i>Oreochromis</i> spp.) |
| title_short | Herbal Vitamin C Prevents DNA Oxidation and Modifies the Metabolomic Water Profile of Tilapia (<i>Oreochromis</i> spp.) |
| title_sort | herbal vitamin c prevents dna oxidation and modifies the metabolomic water profile of tilapia i oreochromis i spp |
| topic | herbal vitamin C DNA damage tilapia |
| url | https://www.mdpi.com/2075-1729/12/8/1243 |
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