Zinc transporter Slc30a1 regulates melanocyte development by interacting with mt2 in zebrafish
The essential trace element zinc is involved in multiple biological processes including development and metabolism, while its role in melanocyte formation is still unclear. Slc30a1a and Slc30a1b are zinc exporters in zebrafish. Here, we found that melanocytes were increased in slc30a1a and slc30a1b...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | European Journal of Cell Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933522000759 |
| _version_ | 1811178852890705920 |
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| author | Zhidan Xia Xiu Yang Xinying Bi Xiujuan Tong Junxia Min Fudi Wang |
| author_facet | Zhidan Xia Xiu Yang Xinying Bi Xiujuan Tong Junxia Min Fudi Wang |
| author_sort | Zhidan Xia |
| collection | DOAJ |
| description | The essential trace element zinc is involved in multiple biological processes including development and metabolism, while its role in melanocyte formation is still unclear. Slc30a1a and Slc30a1b are zinc exporters in zebrafish. Here, we found that melanocytes were increased in slc30a1a and slc30a1b double mutant zebrafish. SMART-seq data revealed that genes involved in the melanoma pathway and the gene mt2, which encodes zinc-binding protein, were significantly upregulated in the mutants. In addition, the expression of mt2 was specifically increased in mutant melanocytes, as detected by in situ hybridization, suggesting an essential role of this gene in the tissue. Mechanistically, we demonstrated that elevated zinc levels resulting from Slc30a1 deficiency promoted melanocyte proliferation and that mt2 played a protective role in the process of Slc30a1/zinc-mediated melanocyte hyperplasia. This study uncovered the critical function of Slc30a1-mediated zinc homeostasis in melanocyte development and suggests that accumulated zinc in melanocytes would be a risk for inducing melanoma and that mt2 is a potential target for controlling diseases related to abnormal melanocyte development. |
| first_indexed | 2024-04-11T06:24:48Z |
| format | Article |
| id | doaj.art-2a1b63613271457c9e24402e0992683b |
| institution | Directory Open Access Journal |
| issn | 0171-9335 |
| language | English |
| last_indexed | 2024-04-11T06:24:48Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj.art-2a1b63613271457c9e24402e0992683b2022-12-22T04:40:24ZengElsevierEuropean Journal of Cell Biology0171-93352022-09-011014151272Zinc transporter Slc30a1 regulates melanocyte development by interacting with mt2 in zebrafishZhidan Xia0Xiu Yang1Xinying Bi2Xiujuan Tong3Junxia Min4Fudi Wang5Corresponding authors.; The Fourth Affiliated Hospital, School of Public Health, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaThe Fourth Affiliated Hospital, School of Public Health, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaThe Fourth Affiliated Hospital, School of Public Health, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaThe Fourth Affiliated Hospital, School of Public Health, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaThe Fourth Affiliated Hospital, School of Public Health, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaCorresponding authors.; The Fourth Affiliated Hospital, School of Public Health, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaThe essential trace element zinc is involved in multiple biological processes including development and metabolism, while its role in melanocyte formation is still unclear. Slc30a1a and Slc30a1b are zinc exporters in zebrafish. Here, we found that melanocytes were increased in slc30a1a and slc30a1b double mutant zebrafish. SMART-seq data revealed that genes involved in the melanoma pathway and the gene mt2, which encodes zinc-binding protein, were significantly upregulated in the mutants. In addition, the expression of mt2 was specifically increased in mutant melanocytes, as detected by in situ hybridization, suggesting an essential role of this gene in the tissue. Mechanistically, we demonstrated that elevated zinc levels resulting from Slc30a1 deficiency promoted melanocyte proliferation and that mt2 played a protective role in the process of Slc30a1/zinc-mediated melanocyte hyperplasia. This study uncovered the critical function of Slc30a1-mediated zinc homeostasis in melanocyte development and suggests that accumulated zinc in melanocytes would be a risk for inducing melanoma and that mt2 is a potential target for controlling diseases related to abnormal melanocyte development.http://www.sciencedirect.com/science/article/pii/S0171933522000759Slc30a1mt2MelanocyteZinc homeostasisZebrafish |
| spellingShingle | Zhidan Xia Xiu Yang Xinying Bi Xiujuan Tong Junxia Min Fudi Wang Zinc transporter Slc30a1 regulates melanocyte development by interacting with mt2 in zebrafish European Journal of Cell Biology Slc30a1 mt2 Melanocyte Zinc homeostasis Zebrafish |
| title | Zinc transporter Slc30a1 regulates melanocyte development by interacting with mt2 in zebrafish |
| title_full | Zinc transporter Slc30a1 regulates melanocyte development by interacting with mt2 in zebrafish |
| title_fullStr | Zinc transporter Slc30a1 regulates melanocyte development by interacting with mt2 in zebrafish |
| title_full_unstemmed | Zinc transporter Slc30a1 regulates melanocyte development by interacting with mt2 in zebrafish |
| title_short | Zinc transporter Slc30a1 regulates melanocyte development by interacting with mt2 in zebrafish |
| title_sort | zinc transporter slc30a1 regulates melanocyte development by interacting with mt2 in zebrafish |
| topic | Slc30a1 mt2 Melanocyte Zinc homeostasis Zebrafish |
| url | http://www.sciencedirect.com/science/article/pii/S0171933522000759 |
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