Material and nanomechanical properties of bone structural units of cortical and trabecular iliac bone tissues from untreated postmenopausal osteoporotic women
The differences in bone nanomechanical properties between cortical (Ct) and trabecular (Tb) bone remain uncertain, whereas knowing the respective contribution of each compartment is critical to understand the origin of bone strength. Our purpose was to compare bone mechanical and intrinsic propertie...
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| Language: | English |
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Elsevier
2022-12-01
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| Series: | Bone Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352187222004570 |
| _version_ | 1811201961702195200 |
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| author | Delphine Farlay Guillaume Falgayrac Camille Ponçon Sébastien Rizzo Bernard Cortet Roland Chapurlat Guillaume Penel Isabelle Badoud Patrick Ammann Georges Boivin |
| author_facet | Delphine Farlay Guillaume Falgayrac Camille Ponçon Sébastien Rizzo Bernard Cortet Roland Chapurlat Guillaume Penel Isabelle Badoud Patrick Ammann Georges Boivin |
| author_sort | Delphine Farlay |
| collection | DOAJ |
| description | The differences in bone nanomechanical properties between cortical (Ct) and trabecular (Tb) bone remain uncertain, whereas knowing the respective contribution of each compartment is critical to understand the origin of bone strength. Our purpose was to compare bone mechanical and intrinsic properties of Ct and Tb compartments, at the bone structural unit (BSU) level, in iliac bone taken from a homogeneous untreated human population.Among 60 PMMA-embedded transiliac bone biopsies from untreated postmenopausal osteoporotic women (64 ± 7 year-old), >2000 BSUs were analysed by nanoindentation in physiological wet conditions [indentation modulus (elasticity), hardness, dissipated energy], by Fourier transform infrared (FTIRM) and Raman microspectroscopy (mineral and organic characteristics), and by X-ray microradiography (degree of mineralization of bone, DMB). BSUs were categorized based on tissue age, osteonal (Ost) and interstitial (Int) tissues location and bone compartments (Ct and Tb).Indentation modulus was higher in Ct than in Tb BSUs, both in Ost and Int. dissipated energy was higher in Ct than Tb, in Int BSUs. Hardness was not different between Ct and Tb BSUs. In Ost or Int BSUs, mineral maturity (conversion of non-apatitic into apatitic phosphates) was higher in Ct than in Tb, as well as for collagen maturity (Ost). Mineral content assessed as mineral/matrix (FTIRM and Raman) or as DMB, was lower in Ct than in Tb. Crystallinity (FTIRM) was similar in BSUs from Ct and Tb, and slightly lower in Ct than in Tb when measured by Raman, indicating that the crystal size/perfection was quite similar between Ct and Tb BSUs. The differences found between Ost and Int tissues were much higher than the difference found between Ct and Tb for all those bone material properties. Multiple regression analysis showed that Indentation modulus and dissipated energy were mainly explained by mineral maturity in Ct and by collagen maturity in Tb, and hardness by mineral content in both Ct and Tb.In conclusion, in untreated human iliac bone, Ct and Tb BSUs exhibit different characteristics. Ct BSUs have higher indentation modulus, dissipated energy (Int), mineral and organic maturities than Tb BSUs, without difference in hardness. Although those differences are relatively small compared to those found between Ost and Int BSUs, they may influence bone strength at macroscale. |
| first_indexed | 2024-04-12T02:31:52Z |
| format | Article |
| id | doaj.art-2a1ecd5f85ed47dc9a93785e4e3fd943 |
| institution | Directory Open Access Journal |
| issn | 2352-1872 |
| language | English |
| last_indexed | 2024-04-12T02:31:52Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
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| series | Bone Reports |
| spelling | doaj.art-2a1ecd5f85ed47dc9a93785e4e3fd9432022-12-22T03:51:45ZengElsevierBone Reports2352-18722022-12-0117101623Material and nanomechanical properties of bone structural units of cortical and trabecular iliac bone tissues from untreated postmenopausal osteoporotic womenDelphine Farlay0Guillaume Falgayrac1Camille Ponçon2Sébastien Rizzo3Bernard Cortet4Roland Chapurlat5Guillaume Penel6Isabelle Badoud7Patrick Ammann8Georges Boivin9INSERM, UMR 1033, Univ Lyon, Université Claude Bernard Lyon1, F-69008 Lyon, France; Corresponding author.Univ. Lille, CHU Lille, Univ. Littoral Côte d'Opale, ULR 4490 - MABLab- Adiposité, Médullaire et Os, F-59000 Lille, FranceINSERM, UMR 1033, Univ Lyon, Université Claude Bernard Lyon1, F-69008 Lyon, FranceINSERM, UMR 1033, Univ Lyon, Université Claude Bernard Lyon1, F-69008 Lyon, FranceUniv. Lille, CHU Lille, Univ. Littoral Côte d'Opale, ULR 4490 - MABLab- Adiposité, Médullaire et Os, F-59000 Lille, FranceINSERM, UMR 1033, Univ Lyon, Université Claude Bernard Lyon1, F-69008 Lyon, FranceUniv. Lille, CHU Lille, Univ. Littoral Côte d'Opale, ULR 4490 - MABLab- Adiposité, Médullaire et Os, F-59000 Lille, FranceDivision of Bone Diseases, Department of Internal Medicine Specialties, Geneva University Hospital, 4rue Gabrielle-Perret-Gentil, CH-1211 Geneva 14, SwitzerlandDivision of Bone Diseases, Department of Internal Medicine Specialties, Geneva University Hospital, 4rue Gabrielle-Perret-Gentil, CH-1211 Geneva 14, SwitzerlandINSERM, UMR 1033, Univ Lyon, Université Claude Bernard Lyon1, F-69008 Lyon, FranceThe differences in bone nanomechanical properties between cortical (Ct) and trabecular (Tb) bone remain uncertain, whereas knowing the respective contribution of each compartment is critical to understand the origin of bone strength. Our purpose was to compare bone mechanical and intrinsic properties of Ct and Tb compartments, at the bone structural unit (BSU) level, in iliac bone taken from a homogeneous untreated human population.Among 60 PMMA-embedded transiliac bone biopsies from untreated postmenopausal osteoporotic women (64 ± 7 year-old), >2000 BSUs were analysed by nanoindentation in physiological wet conditions [indentation modulus (elasticity), hardness, dissipated energy], by Fourier transform infrared (FTIRM) and Raman microspectroscopy (mineral and organic characteristics), and by X-ray microradiography (degree of mineralization of bone, DMB). BSUs were categorized based on tissue age, osteonal (Ost) and interstitial (Int) tissues location and bone compartments (Ct and Tb).Indentation modulus was higher in Ct than in Tb BSUs, both in Ost and Int. dissipated energy was higher in Ct than Tb, in Int BSUs. Hardness was not different between Ct and Tb BSUs. In Ost or Int BSUs, mineral maturity (conversion of non-apatitic into apatitic phosphates) was higher in Ct than in Tb, as well as for collagen maturity (Ost). Mineral content assessed as mineral/matrix (FTIRM and Raman) or as DMB, was lower in Ct than in Tb. Crystallinity (FTIRM) was similar in BSUs from Ct and Tb, and slightly lower in Ct than in Tb when measured by Raman, indicating that the crystal size/perfection was quite similar between Ct and Tb BSUs. The differences found between Ost and Int tissues were much higher than the difference found between Ct and Tb for all those bone material properties. Multiple regression analysis showed that Indentation modulus and dissipated energy were mainly explained by mineral maturity in Ct and by collagen maturity in Tb, and hardness by mineral content in both Ct and Tb.In conclusion, in untreated human iliac bone, Ct and Tb BSUs exhibit different characteristics. Ct BSUs have higher indentation modulus, dissipated energy (Int), mineral and organic maturities than Tb BSUs, without difference in hardness. Although those differences are relatively small compared to those found between Ost and Int BSUs, they may influence bone strength at macroscale.http://www.sciencedirect.com/science/article/pii/S2352187222004570Bone intrinsic propertiesNanomechanical propertiesCortical/trabecular boneNanoindentationInfrared spectroscopyRaman spectroscopy |
| spellingShingle | Delphine Farlay Guillaume Falgayrac Camille Ponçon Sébastien Rizzo Bernard Cortet Roland Chapurlat Guillaume Penel Isabelle Badoud Patrick Ammann Georges Boivin Material and nanomechanical properties of bone structural units of cortical and trabecular iliac bone tissues from untreated postmenopausal osteoporotic women Bone Reports Bone intrinsic properties Nanomechanical properties Cortical/trabecular bone Nanoindentation Infrared spectroscopy Raman spectroscopy |
| title | Material and nanomechanical properties of bone structural units of cortical and trabecular iliac bone tissues from untreated postmenopausal osteoporotic women |
| title_full | Material and nanomechanical properties of bone structural units of cortical and trabecular iliac bone tissues from untreated postmenopausal osteoporotic women |
| title_fullStr | Material and nanomechanical properties of bone structural units of cortical and trabecular iliac bone tissues from untreated postmenopausal osteoporotic women |
| title_full_unstemmed | Material and nanomechanical properties of bone structural units of cortical and trabecular iliac bone tissues from untreated postmenopausal osteoporotic women |
| title_short | Material and nanomechanical properties of bone structural units of cortical and trabecular iliac bone tissues from untreated postmenopausal osteoporotic women |
| title_sort | material and nanomechanical properties of bone structural units of cortical and trabecular iliac bone tissues from untreated postmenopausal osteoporotic women |
| topic | Bone intrinsic properties Nanomechanical properties Cortical/trabecular bone Nanoindentation Infrared spectroscopy Raman spectroscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2352187222004570 |
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