Modules for in vitro metabolic engineering: Pathway assembly for bio-based production of value-added chemicals
Bio-based chemical production has drawn attention regarding the realization of a sustainable society. In vitro metabolic engineering is one of the methods used for the bio-based production of value-added chemicals. This method involves the reconstitution of natural or artificial metabolic pathways b...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2017-06-01
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| Series: | Synthetic and Systems Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X17300182 |
| _version_ | 1797205340055404544 |
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| author | Hironori Taniguchi Kenji Okano Kohsuke Honda |
| author_facet | Hironori Taniguchi Kenji Okano Kohsuke Honda |
| author_sort | Hironori Taniguchi |
| collection | DOAJ |
| description | Bio-based chemical production has drawn attention regarding the realization of a sustainable society. In vitro metabolic engineering is one of the methods used for the bio-based production of value-added chemicals. This method involves the reconstitution of natural or artificial metabolic pathways by assembling purified/semi-purified enzymes in vitro. Enzymes from distinct sources can be combined to construct desired reaction cascades with fewer biological constraints in one vessel, enabling easier pathway design with high modularity. Multiple modules have been designed, built, tested, and improved by different groups for different purpose. In this review, we focus on these in vitro metabolic engineering modules, especially focusing on the carbon metabolism, and present an overview of input modules, output modules, and other modules related to cofactor management. |
| first_indexed | 2024-04-24T08:49:34Z |
| format | Article |
| id | doaj.art-2a1f19b857bc4f32a199e6144843ac86 |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2024-04-24T08:49:34Z |
| publishDate | 2017-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-2a1f19b857bc4f32a199e6144843ac862024-04-16T12:12:30ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2017-06-0122657410.1016/j.synbio.2017.06.002Modules for in vitro metabolic engineering: Pathway assembly for bio-based production of value-added chemicalsHironori TaniguchiKenji OkanoKohsuke HondaBio-based chemical production has drawn attention regarding the realization of a sustainable society. In vitro metabolic engineering is one of the methods used for the bio-based production of value-added chemicals. This method involves the reconstitution of natural or artificial metabolic pathways by assembling purified/semi-purified enzymes in vitro. Enzymes from distinct sources can be combined to construct desired reaction cascades with fewer biological constraints in one vessel, enabling easier pathway design with high modularity. Multiple modules have been designed, built, tested, and improved by different groups for different purpose. In this review, we focus on these in vitro metabolic engineering modules, especially focusing on the carbon metabolism, and present an overview of input modules, output modules, and other modules related to cofactor management.http://www.sciencedirect.com/science/article/pii/S2405805X17300182In vitro metabolic engineeringModuleGlycolysisPentose phosphate pathwayCofactor management |
| spellingShingle | Hironori Taniguchi Kenji Okano Kohsuke Honda Modules for in vitro metabolic engineering: Pathway assembly for bio-based production of value-added chemicals Synthetic and Systems Biotechnology In vitro metabolic engineering Module Glycolysis Pentose phosphate pathway Cofactor management |
| title | Modules for in vitro metabolic engineering: Pathway assembly for bio-based production of value-added chemicals |
| title_full | Modules for in vitro metabolic engineering: Pathway assembly for bio-based production of value-added chemicals |
| title_fullStr | Modules for in vitro metabolic engineering: Pathway assembly for bio-based production of value-added chemicals |
| title_full_unstemmed | Modules for in vitro metabolic engineering: Pathway assembly for bio-based production of value-added chemicals |
| title_short | Modules for in vitro metabolic engineering: Pathway assembly for bio-based production of value-added chemicals |
| title_sort | modules for in vitro metabolic engineering pathway assembly for bio based production of value added chemicals |
| topic | In vitro metabolic engineering Module Glycolysis Pentose phosphate pathway Cofactor management |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X17300182 |
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