Understanding the early cold response mechanism in IR64 indica rice variety through comparative transcriptome analysis
Abstract Background Cellular reprogramming in response to environmental stress involves alteration of gene expression, changes in the protein and metabolite profile for ensuring better stress management in plants. Similar to other plant species originating in tropical and sub-tropical areas, indica...
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-020-06841-2 |
| _version_ | 1811339545144197120 |
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| author | Pratiti Dasgupta Abhishek Das Sambit Datta Ishani Banerjee Sucheta Tripathy Shubho Chaudhuri |
| author_facet | Pratiti Dasgupta Abhishek Das Sambit Datta Ishani Banerjee Sucheta Tripathy Shubho Chaudhuri |
| author_sort | Pratiti Dasgupta |
| collection | DOAJ |
| description | Abstract Background Cellular reprogramming in response to environmental stress involves alteration of gene expression, changes in the protein and metabolite profile for ensuring better stress management in plants. Similar to other plant species originating in tropical and sub-tropical areas, indica rice is highly sensitive to low temperature that adversely affects its growth and grain productivity. Substantial work has been done to understand cold induced changes in gene expression in rice plants. However, adequate information is not available for early gene expression, especially in indica variety. Therefore, a transcriptome profile was generated for cold shock treated seedlings of IR64 variety to identify early responsive genes. Results The functional annotation of early DEGs shows enrichment of genes involved in altered membrane rigidity and electrolytic leakage, the onset of calcium signaling, ROS generation and activation of stress responsive transcription factors in IR64. Gene regulatory network suggests that cold shock induced Ca2+ signaling activates DREB/CBF pathway and other groups of transcription factors such as MYB, NAC and ZFP; for activating various cold-responsive genes. The analysis also indicates that cold induced signaling proteins like RLKs, RLCKs, CDPKs and MAPKK and ROS signaling proteins. Further, several late-embryogenesis-abundant (LEA), dehydrins and low temperature-induced-genes were upregulated under early cold shock condition, indicating the onset of water-deficit conditions. Expression profiling in different high yielding cultivars shows high expression of cold-responsive genes in Heera and CB1 indica varieties. These varieties show low levels of cold induced ROS production, electrolytic leakage and high germination rate post-cold stress, compared to IR36 and IR64. Collectively, these results suggest that these varieties may have improved adaptability to cold stress. Conclusions The results of this study provide insights about early responsive events in Oryza sativa l.ssp. indica cv IR64 in response to cold stress. Our data shows the onset of cold response is associated with upregulation of stress responsive TFs, hydrophilic proteins and signaling molecules, whereas, the genes coding for cellular biosynthetic enzymes, cell cycle control and growth-related TFs are downregulated. This study reports that the generation of ROS is integral to the early response to trigger the ROS mediated signaling events during later stages. |
| first_indexed | 2024-04-13T18:28:17Z |
| format | Article |
| id | doaj.art-2a21b79c998b4e43b215a0ec1b7e8f1e |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-13T18:28:17Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-2a21b79c998b4e43b215a0ec1b7e8f1e2022-12-22T02:35:11ZengBMCBMC Genomics1471-21642020-06-0121111810.1186/s12864-020-06841-2Understanding the early cold response mechanism in IR64 indica rice variety through comparative transcriptome analysisPratiti Dasgupta0Abhishek Das1Sambit Datta2Ishani Banerjee3Sucheta Tripathy4Shubho Chaudhuri5Division of Plant Biology, Bose InstituteStructural Biology & Bioinformatics Division, CSIR- Indian Institute of Chemical BiologyDivision of Plant Biology, Bose InstituteDivision of Plant Biology, Bose InstituteStructural Biology & Bioinformatics Division, CSIR- Indian Institute of Chemical BiologyDivision of Plant Biology, Bose InstituteAbstract Background Cellular reprogramming in response to environmental stress involves alteration of gene expression, changes in the protein and metabolite profile for ensuring better stress management in plants. Similar to other plant species originating in tropical and sub-tropical areas, indica rice is highly sensitive to low temperature that adversely affects its growth and grain productivity. Substantial work has been done to understand cold induced changes in gene expression in rice plants. However, adequate information is not available for early gene expression, especially in indica variety. Therefore, a transcriptome profile was generated for cold shock treated seedlings of IR64 variety to identify early responsive genes. Results The functional annotation of early DEGs shows enrichment of genes involved in altered membrane rigidity and electrolytic leakage, the onset of calcium signaling, ROS generation and activation of stress responsive transcription factors in IR64. Gene regulatory network suggests that cold shock induced Ca2+ signaling activates DREB/CBF pathway and other groups of transcription factors such as MYB, NAC and ZFP; for activating various cold-responsive genes. The analysis also indicates that cold induced signaling proteins like RLKs, RLCKs, CDPKs and MAPKK and ROS signaling proteins. Further, several late-embryogenesis-abundant (LEA), dehydrins and low temperature-induced-genes were upregulated under early cold shock condition, indicating the onset of water-deficit conditions. Expression profiling in different high yielding cultivars shows high expression of cold-responsive genes in Heera and CB1 indica varieties. These varieties show low levels of cold induced ROS production, electrolytic leakage and high germination rate post-cold stress, compared to IR36 and IR64. Collectively, these results suggest that these varieties may have improved adaptability to cold stress. Conclusions The results of this study provide insights about early responsive events in Oryza sativa l.ssp. indica cv IR64 in response to cold stress. Our data shows the onset of cold response is associated with upregulation of stress responsive TFs, hydrophilic proteins and signaling molecules, whereas, the genes coding for cellular biosynthetic enzymes, cell cycle control and growth-related TFs are downregulated. This study reports that the generation of ROS is integral to the early response to trigger the ROS mediated signaling events during later stages.http://link.springer.com/article/10.1186/s12864-020-06841-2IR64Cold shockTranscriptomeDifferentially expressed genes (DEGs)Transcription factors (TFs)Calcium signaling |
| spellingShingle | Pratiti Dasgupta Abhishek Das Sambit Datta Ishani Banerjee Sucheta Tripathy Shubho Chaudhuri Understanding the early cold response mechanism in IR64 indica rice variety through comparative transcriptome analysis BMC Genomics IR64 Cold shock Transcriptome Differentially expressed genes (DEGs) Transcription factors (TFs) Calcium signaling |
| title | Understanding the early cold response mechanism in IR64 indica rice variety through comparative transcriptome analysis |
| title_full | Understanding the early cold response mechanism in IR64 indica rice variety through comparative transcriptome analysis |
| title_fullStr | Understanding the early cold response mechanism in IR64 indica rice variety through comparative transcriptome analysis |
| title_full_unstemmed | Understanding the early cold response mechanism in IR64 indica rice variety through comparative transcriptome analysis |
| title_short | Understanding the early cold response mechanism in IR64 indica rice variety through comparative transcriptome analysis |
| title_sort | understanding the early cold response mechanism in ir64 indica rice variety through comparative transcriptome analysis |
| topic | IR64 Cold shock Transcriptome Differentially expressed genes (DEGs) Transcription factors (TFs) Calcium signaling |
| url | http://link.springer.com/article/10.1186/s12864-020-06841-2 |
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