Molecular prevalence and factors associated with Ehrlichia canis infection in dogs from the North Pantanal wetland, Brazil
Background and Aim: Canine monocytic ehrlichiosis is a vector-borne disease caused by the obligatory intracellular bacterium Ehrlichia canis, which is distributed across tropical and subtropical regions worldwide. Its prevalence within dog populations is high in municipalities located across the Pan...
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| Format: | Article |
| Language: | English |
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Veterinary World
2023-06-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.16/June-2023/4.pdf |
| _version_ | 1827932748052955136 |
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| author | Mariana Elisa Pereira Darlan Henrique Canei Matheus Roberto Carvalho Álvaro Felipe de Lima Ruy Dias Arleana do Bom Parto Ferreira de Almeida Luciano Nakazato Valéria Régia Franco Sousa |
| author_facet | Mariana Elisa Pereira Darlan Henrique Canei Matheus Roberto Carvalho Álvaro Felipe de Lima Ruy Dias Arleana do Bom Parto Ferreira de Almeida Luciano Nakazato Valéria Régia Franco Sousa |
| author_sort | Mariana Elisa Pereira |
| collection | DOAJ |
| description | Background and Aim: Canine monocytic ehrlichiosis is a vector-borne disease caused by the obligatory intracellular bacterium Ehrlichia canis, which is distributed across tropical and subtropical regions worldwide. Its prevalence within dog populations is high in municipalities located across the Pantanal biome, but it remains unknown in Barão de Melgaço, Mato Grosso, Brazil. This study aimed to determine the molecular prevalence and factors associated with E. canis infection in dogs domiciled in Barão de Melgaço.
Materials and Methods: A cross-sectional study was carried out to investigate the prevalence of E. canis infection in 369 dogs from urban and rural areas in Barão de Melgaço, North Pantanal wetland, Brazil. Initially, the dogs were examined, and, through a questionnaire, the risk factors were investigated. Blood samples were subjected to DNA extraction and PCR was performed to estimate the prevalence of E. canis infection.
Results: The molecular prevalence of E. canis infection in dogs was 42.5% and none of the studied variables were significantly associated with polymerase chain reaction (PCR) positivity (p > 0.05).
Conclusion: The high molecular prevalence demonstrates an increased transmission of the agent across the city. This also indicates that attention needs to be paid to E. canis infection and control measures should be introduced to prevent its transmission. The demographic and clinical risk factors commonly associated with E. canis infection in this study were not associated with PCR positivity. |
| first_indexed | 2024-03-13T07:13:41Z |
| format | Article |
| id | doaj.art-2a22ad2d836d4c22a276fd571e416b52 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-03-13T07:13:41Z |
| publishDate | 2023-06-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-2a22ad2d836d4c22a276fd571e416b522023-06-05T11:56:11ZengVeterinary WorldVeterinary World0972-89882231-09162023-06-011661209121310.14202/vetworld.2023.1209-1213Molecular prevalence and factors associated with Ehrlichia canis infection in dogs from the North Pantanal wetland, BrazilMariana Elisa Pereira0https://orcid.org/0000-0003-1661-9898Darlan Henrique Canei1https://orcid.org/0000-0001-9164-4685Matheus Roberto Carvalho2https://orcid.org/0000-0002-3149-6139Álvaro Felipe de Lima Ruy Dias3https://orcid.org/0000-0002-8303-6302Arleana do Bom Parto Ferreira de Almeida4https://orcid.org/0000-0002-6694-1407Luciano Nakazato5https://orcid.org/0000-0002-1244-0690Valéria Régia Franco Sousa6https://orcid.org/0000-0001-6209-4514Postgraduate Program in Veterinary Science, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Avenue Fernando Correa da Costa, Boa Esperança, Cuiabá, Mato Grosso, Brazil.Postgraduate Program in Veterinary Science, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Avenue Fernando Correa da Costa, Boa Esperança, Cuiabá, Mato Grosso, Brazil.Postgraduate Program in Veterinary Science, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Avenue Fernando Correa da Costa, Boa Esperança, Cuiabá, Mato Grosso, Brazil.Postgraduate Program in Veterinary Science, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Avenue Fernando Correa da Costa, Boa Esperança, Cuiabá, Mato Grosso, Brazil.Faculty of Veterinary Medicine, Federal University of Mato Grosso, Avenue Fernando Correa da Costa, Boa Esperança, Cuiabá, Mato Grosso, Brazil.Faculty of Veterinary Medicine, Federal University of Mato Grosso, Avenue Fernando Correa da Costa, Boa Esperança, Cuiabá, Mato Grosso, Brazil.Faculty of Veterinary Medicine, Federal University of Mato Grosso, Avenue Fernando Correa da Costa, Boa Esperança, Cuiabá, Mato Grosso, Brazil.Background and Aim: Canine monocytic ehrlichiosis is a vector-borne disease caused by the obligatory intracellular bacterium Ehrlichia canis, which is distributed across tropical and subtropical regions worldwide. Its prevalence within dog populations is high in municipalities located across the Pantanal biome, but it remains unknown in Barão de Melgaço, Mato Grosso, Brazil. This study aimed to determine the molecular prevalence and factors associated with E. canis infection in dogs domiciled in Barão de Melgaço. Materials and Methods: A cross-sectional study was carried out to investigate the prevalence of E. canis infection in 369 dogs from urban and rural areas in Barão de Melgaço, North Pantanal wetland, Brazil. Initially, the dogs were examined, and, through a questionnaire, the risk factors were investigated. Blood samples were subjected to DNA extraction and PCR was performed to estimate the prevalence of E. canis infection. Results: The molecular prevalence of E. canis infection in dogs was 42.5% and none of the studied variables were significantly associated with polymerase chain reaction (PCR) positivity (p > 0.05). Conclusion: The high molecular prevalence demonstrates an increased transmission of the agent across the city. This also indicates that attention needs to be paid to E. canis infection and control measures should be introduced to prevent its transmission. The demographic and clinical risk factors commonly associated with E. canis infection in this study were not associated with PCR positivity.https://www.veterinaryworld.org/Vol.16/June-2023/4.pdfbacteriumdnadogehrlichiosispantanal |
| spellingShingle | Mariana Elisa Pereira Darlan Henrique Canei Matheus Roberto Carvalho Álvaro Felipe de Lima Ruy Dias Arleana do Bom Parto Ferreira de Almeida Luciano Nakazato Valéria Régia Franco Sousa Molecular prevalence and factors associated with Ehrlichia canis infection in dogs from the North Pantanal wetland, Brazil Veterinary World bacterium dna dog ehrlichiosis pantanal |
| title | Molecular prevalence and factors associated with Ehrlichia canis infection in dogs from the North Pantanal wetland, Brazil |
| title_full | Molecular prevalence and factors associated with Ehrlichia canis infection in dogs from the North Pantanal wetland, Brazil |
| title_fullStr | Molecular prevalence and factors associated with Ehrlichia canis infection in dogs from the North Pantanal wetland, Brazil |
| title_full_unstemmed | Molecular prevalence and factors associated with Ehrlichia canis infection in dogs from the North Pantanal wetland, Brazil |
| title_short | Molecular prevalence and factors associated with Ehrlichia canis infection in dogs from the North Pantanal wetland, Brazil |
| title_sort | molecular prevalence and factors associated with ehrlichia canis infection in dogs from the north pantanal wetland brazil |
| topic | bacterium dna dog ehrlichiosis pantanal |
| url | https://www.veterinaryworld.org/Vol.16/June-2023/4.pdf |
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