Estimation of leaf traits from reflectance measurements: comparison between methods based on vegetation indices and several versions of the PROSPECT model
Abstract Background Leaf biochemical composition corresponds to traits related to the plant state and its functioning. This study puts the emphasis on the main leaf absorbers: chlorophyll a and b ($$C_{ab}$$ Cab ), carotenoids ($$C_{c}$$ Cc ), water ($$C_{w}$$ Cw ) and dry mater ($$C_{m}$$ Cm ) cont...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-03-01
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| Series: | Plant Methods |
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| Online Access: | http://link.springer.com/article/10.1186/s13007-018-0291-x |
| _version_ | 1828810717097099264 |
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| author | Jingyi Jiang Alexis Comar Philippe Burger Pierre Bancal Marie Weiss Frédéric Baret |
| author_facet | Jingyi Jiang Alexis Comar Philippe Burger Pierre Bancal Marie Weiss Frédéric Baret |
| author_sort | Jingyi Jiang |
| collection | DOAJ |
| description | Abstract Background Leaf biochemical composition corresponds to traits related to the plant state and its functioning. This study puts the emphasis on the main leaf absorbers: chlorophyll a and b ($$C_{ab}$$ Cab ), carotenoids ($$C_{c}$$ Cc ), water ($$C_{w}$$ Cw ) and dry mater ($$C_{m}$$ Cm ) contents. Two main approaches were used to estimate [$$C_{ab} ,$$ Cab, $$C_{c}$$ Cc , $$C_{w}$$ Cw , $$C_{m}$$ Cm ] in a non-destructive way using spectral measurements. The first one consists in building empirical relationships from experimental datasets using either the raw reflectances or their combination into vegetation indices (VI). The second one relies on the inversion of physically based models of leaf optical properties. Although the first approach is commonly used, the calibration of the empirical relationships is generally conducted over a limited dataset. Consequently, poor predictions may be observed when applying them on cases that are not represented in the training dataset, i.e. when dealing with different species, genotypes or under contrasted environmental conditions. The retrieval performances of the selected VIs were thus compared to the ones of four PROSPECT model versions based on reflectance data acquired at two phenological stages, over six wheat genotypes grown under three different nitrogen fertilizations and two sowing density modalities. Leaf reflectance was measured in the lab with a spectrophotometer equipped with an integrating sphere, the leaf being placed in front of a white Teflon background to increase the sensitivity to leaf biochemical composition. Destructive measurements of [$$C_{ab} ,$$ Cab, $$C_{c}$$ Cc , $$C_{w}$$ Cw , $$C_{m}$$ Cm ] were performed concurrently. Results The destructive measurements demonstrated that the carotenoid, $$C_{c}$$ Cc , and chlorophyll, $$C_{ab}$$ Cab , contents were strongly correlated (r2 = 0.91). The sum of $$C_{ab}$$ Cab and $$C_{c}$$ Cc , i.e. the total chlorophyllian pigment content, $$C_{abc}$$ Cabc , was therefore used in this study. When inverting the PROSPECT model, accounting for the brown pigment content, $$C_{bp}$$ Cbp , was necessary when leaves started to senesce. The values of $$C_{abc}$$ Cabc and $$C_{w}$$ Cw were well estimated (r2 = 0.81 and r2 = 0.88 respectively) while the dry matter content, $$C_{m}$$ Cm , was poorly estimated (r2 = 0.00). Retrieval of $$C_{w}$$ Cw from PROSPECT versions was only slightly biased, while substantial overestimation of $$C_{abc }$$ Cabc was observed. The ranking between estimated values of $$C_{abc}$$ Cabc and $$C_{w}$$ Cw from the several PROSPECT versions and that derived using the VIs were similar to the ranking observed over the destructively measured values of $$C_{abc}$$ Cabc and $$C_{w}$$ Cw . Conclusions PROSPECT model inversion and empirical VI approach provide similar retrieval performances and are useful methods to estimate leaf biochemical composition from spectral measurements. However, the PROSPECT model inversion gives potential access to additional traits on surface reflectivity and leaf internal structure. This study suggests that non-destructive estimation of leaf chlorophyll and water contents is a relevant method to provide leaf traits with relatively high throughput. |
| first_indexed | 2024-12-12T09:16:05Z |
| format | Article |
| id | doaj.art-2a25c2ccc0f54984a9c2a68197fc80b6 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-12-12T09:16:05Z |
| publishDate | 2018-03-01 |
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| series | Plant Methods |
| spelling | doaj.art-2a25c2ccc0f54984a9c2a68197fc80b62022-12-22T00:29:23ZengBMCPlant Methods1746-48112018-03-0114111610.1186/s13007-018-0291-xEstimation of leaf traits from reflectance measurements: comparison between methods based on vegetation indices and several versions of the PROSPECT modelJingyi Jiang0Alexis Comar1Philippe Burger2Pierre Bancal3Marie Weiss4Frédéric Baret5EMMAH UMR 1114, INRA, UAPVHIPHENAGIR UMR 1248, INRAUMR ECOSYS, INRAEMMAH UMR 1114, INRA, UAPVEMMAH UMR 1114, INRA, UAPVAbstract Background Leaf biochemical composition corresponds to traits related to the plant state and its functioning. This study puts the emphasis on the main leaf absorbers: chlorophyll a and b ($$C_{ab}$$ Cab ), carotenoids ($$C_{c}$$ Cc ), water ($$C_{w}$$ Cw ) and dry mater ($$C_{m}$$ Cm ) contents. Two main approaches were used to estimate [$$C_{ab} ,$$ Cab, $$C_{c}$$ Cc , $$C_{w}$$ Cw , $$C_{m}$$ Cm ] in a non-destructive way using spectral measurements. The first one consists in building empirical relationships from experimental datasets using either the raw reflectances or their combination into vegetation indices (VI). The second one relies on the inversion of physically based models of leaf optical properties. Although the first approach is commonly used, the calibration of the empirical relationships is generally conducted over a limited dataset. Consequently, poor predictions may be observed when applying them on cases that are not represented in the training dataset, i.e. when dealing with different species, genotypes or under contrasted environmental conditions. The retrieval performances of the selected VIs were thus compared to the ones of four PROSPECT model versions based on reflectance data acquired at two phenological stages, over six wheat genotypes grown under three different nitrogen fertilizations and two sowing density modalities. Leaf reflectance was measured in the lab with a spectrophotometer equipped with an integrating sphere, the leaf being placed in front of a white Teflon background to increase the sensitivity to leaf biochemical composition. Destructive measurements of [$$C_{ab} ,$$ Cab, $$C_{c}$$ Cc , $$C_{w}$$ Cw , $$C_{m}$$ Cm ] were performed concurrently. Results The destructive measurements demonstrated that the carotenoid, $$C_{c}$$ Cc , and chlorophyll, $$C_{ab}$$ Cab , contents were strongly correlated (r2 = 0.91). The sum of $$C_{ab}$$ Cab and $$C_{c}$$ Cc , i.e. the total chlorophyllian pigment content, $$C_{abc}$$ Cabc , was therefore used in this study. When inverting the PROSPECT model, accounting for the brown pigment content, $$C_{bp}$$ Cbp , was necessary when leaves started to senesce. The values of $$C_{abc}$$ Cabc and $$C_{w}$$ Cw were well estimated (r2 = 0.81 and r2 = 0.88 respectively) while the dry matter content, $$C_{m}$$ Cm , was poorly estimated (r2 = 0.00). Retrieval of $$C_{w}$$ Cw from PROSPECT versions was only slightly biased, while substantial overestimation of $$C_{abc }$$ Cabc was observed. The ranking between estimated values of $$C_{abc}$$ Cabc and $$C_{w}$$ Cw from the several PROSPECT versions and that derived using the VIs were similar to the ranking observed over the destructively measured values of $$C_{abc}$$ Cabc and $$C_{w}$$ Cw . Conclusions PROSPECT model inversion and empirical VI approach provide similar retrieval performances and are useful methods to estimate leaf biochemical composition from spectral measurements. However, the PROSPECT model inversion gives potential access to additional traits on surface reflectivity and leaf internal structure. This study suggests that non-destructive estimation of leaf chlorophyll and water contents is a relevant method to provide leaf traits with relatively high throughput.http://link.springer.com/article/10.1186/s13007-018-0291-xChlorophyll contentCarotenoid contentWater contentDry matter contentRadiative transfer modelReflectance |
| spellingShingle | Jingyi Jiang Alexis Comar Philippe Burger Pierre Bancal Marie Weiss Frédéric Baret Estimation of leaf traits from reflectance measurements: comparison between methods based on vegetation indices and several versions of the PROSPECT model Plant Methods Chlorophyll content Carotenoid content Water content Dry matter content Radiative transfer model Reflectance |
| title | Estimation of leaf traits from reflectance measurements: comparison between methods based on vegetation indices and several versions of the PROSPECT model |
| title_full | Estimation of leaf traits from reflectance measurements: comparison between methods based on vegetation indices and several versions of the PROSPECT model |
| title_fullStr | Estimation of leaf traits from reflectance measurements: comparison between methods based on vegetation indices and several versions of the PROSPECT model |
| title_full_unstemmed | Estimation of leaf traits from reflectance measurements: comparison between methods based on vegetation indices and several versions of the PROSPECT model |
| title_short | Estimation of leaf traits from reflectance measurements: comparison between methods based on vegetation indices and several versions of the PROSPECT model |
| title_sort | estimation of leaf traits from reflectance measurements comparison between methods based on vegetation indices and several versions of the prospect model |
| topic | Chlorophyll content Carotenoid content Water content Dry matter content Radiative transfer model Reflectance |
| url | http://link.springer.com/article/10.1186/s13007-018-0291-x |
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